Yeast exo-β-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae

Yeast exo-1,3-β-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(β-D -glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and its homologous gene in Candida albicans to function as reporter genes. These open reading frames were coupled to different promoters in multicopy plasmids, and exoglucanase activity quantified at flow cytometry. Exoglucanases were found to be useful tools for the study of promoter regions in S. cerevisiae. This technique has the advantage over other reporter gene systems—such as β-galactosidase fusions—that it does not require permeabilization of yeast cells and therefore it allows the recovery of viable cells—by sorting—after flow cytometry analysis.     

Marmaray project: Tunnels and stations in BC contract

Istanbul is one of the most famous historical cities in the world. However, the project alignment selected as the best of a range of alignments cannot avoid passing beneath the historical and cultural heritages of Istanbul as well as under ancient and densely inhabited areas of the city. This paper will explain some of the challenges related to the bored tunnels.

Historical buildings are vulnerable. Yet many existing residential and office buildings are old and constructed on minimal foundations. As a consequence, it is vital that any drawdown of groundwater and any ground settlements have to be minimized.

In addition, the connection between the immersed and bored tunnels will be made directly and totally underground, without the usual intermediate shafts and beneath the deep waters of the Bosphorus Strait. This operation needs the utmost control of the tunnel excavation face to ensure its stability and to minimize water ingress. Based on such considerations, tunnel excavation by tunnel boring machine (TBM) using a slurry shield and having the ability to operate under high pore pressures was recommended as the method of excavation for the main running tunnels.

The paper will explain how the design team from Avrasyaconsult – the Employer’s Representative – arrived at the final minimum, specific and functional requirements of the bored tunneling works which are to be carried out using the ‘FIDIC EPC/Turnkey Project’ conditions.     

当前我国在学位价值取向上的错位现象及其原因透视

学位具有学术价值、教育价值、管理价值等本体性价值，也具有个体价值与社会价值、工具性价值和目的性价值等派生性价值。当前人们在学位价值取向上存在着一些明显的错位和偏差。其具体表现有四点：一是重学位的“派生”性价值，轻学位的“本体”性价值；二是过分强调学位的教育价值和管理价值，而缺乏对学术价值应有地位的肯定；三是重学位的社会价值，轻学位的个体价值；四是重学位的工具性价值而忽视学位的目的性价值。导致这种错位现象产生的原因，不仅有教育系统内部因素，而且也与中国传统文化价值观和当前社会对人才的选拔、聘用政策有着直接的关系。     

基于手绘艺术作品的文化创意服务与交易平台的分析与设计

进入21世纪,文化创意产业从概念逐步变为现实,也带来了巨大的经济效益。而文化创意产业不断产生新的文化创意作品,如何把这些文化创意作品更好的为社会服务?如何进行文化创意作品的在线交易?本文重点以手绘艺术作品为例进行了研究,通过制作手绘艺术作品的网站从而实现在线交易作品的目的。     

经典传说的新语境分析:以牛郎织女传说为例

牛郎织女传说作为我国四大传说之一,在历史传承中发生了文化变迁,其影响日益式微。在当代语境下,后现代主义思潮的推动,新科技手段的应用,为牛郎织女传说的传承提供了新的机遇。本文拟就对牛郎织女历史语境及当代语境作出分析,同时对牛郎织女未来保护与传承进行构想与展望,思考牛郎织女传说的发展辙迹。     

大历史视野下中国饮食走进西方世界的文化解读——英国作家J．A．G．罗伯茨《东食西渐》评析

英国作家J．A．G．罗伯茨先生的《东食西渐》一书,是从一个真正意义上的西方人的视角,运用丰富翔实的英文书籍资料,包括一些珍贵的历史图片撰写而成,而且这些资料在中国国内几乎是找不到的。他从一个生活在中国本土的中国学者无法做到的研究角度,对中国饮食文化自18世纪以来在西方的传播渗透与普及流行进行了深度的介绍与揭示。其深远的文化意义与历史贡献是不言而喻的。     

文化阻隔和语义差异对翻译的影响

由于文化差异的存在,文化阻隔和语义差异随处可见,存在于世界的每个角落,这就为翻译工作者造成了很大的障碍。通过对文化阻隔和语义差异的分析、研究,指出翻译时应当注意避免的问题。     

中山纪念堂屋面渗漏原因分析与治理方法

分析了中山纪念堂屋面的构造和渗漏的原因,介绍了屋面的维修原则和具体的整治方法。     

Review of Evolution, culture and the human mind.

Reviews the book, Evolution, culture and the human mind edited by M. Schaller, et al. (see record 2009-20004-000). This current volume, Evolution, culture and the human mind, follows the lead of Wundt where an attempt is made to provide a synthesis of theory and data across psychological subfields into a holistic framework. Norenzayan, Schaller, and Heine begin the volume with an overview of the scope and themes that arose from their 2004 conference at the University of British Columbia on “Mind, Culture and Evolution.” Explicit throughout this volume is an effort to bridge the “yawning chasm” between perspectives of evolutionary determinism and cultural constructionism. In doing so, there is interest in providing a rigorous multidisciplinary scientific effort to solve this foundational problem for psychology. As such this volume provides an interesting and insightful examination of the evolution of consciousness, cognition, decision-making, actions, and cultural norms in terms of collective consequences and genetic mechanisms. The volume is divided into three sections where several dialectical themes (theory/data, top-down/bottom-up, ecological/social, diversification/integration) are woven throughout. Overall the volume offers nice flow from one collection of essays to the next as themes are picked up and let go, only to return later on. This volume shows a dominance of natural science (cross-cultural) inclinations where some readers may call for more voice given to phenomenological and hermeneutical (cultural) human science contributions. Other critics may challenge the reliance upon correlational, comparative, and post hoc data to bolster claims of causality in support of various theoretical suppositions. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

现代产品设计中的区域文化意象研究

区域文化意象的研究,有助于体现产品区域特色和文化内涵.研究了区域文化意象的消费者认知匹配模型,阐述了区域文化意象的消费者认知匹配模型的3个层次.并以良渚文化意象的酒器设计为例进行验证.