Automated fluorimetry in quality control of pasteurized and ultra-high temperature-treated starch soup

Automated monitoring of the microbiological quality of heat-processed foods by the resazurin reduction test was applied to microtitration plate incubator-fluorimeter technology. The appearance and disappearance of the fluorescing peak of resorufin was monitored on microtitration trays. Pasteurized or ultra-high temperature-treated starch-based soup was used as the model food system. Bacillus subtilis spores (ultra-high temperature treatment) and vegetative cells of Enterococcus faecalis (pasteurization) were inoculated into the soup before the heat treatment at levels which resulted in some survival. The timing of appearance of maximum fluorescence correlated with the number of bacteria in pre-incubated samples. Automated resazurin-reduction fluorimetry was compared with conventional plating, turbidometry and microcolony count by the direct epifluorescent filter technique. The results of the resazurin test correlated well with those of all the other methods tested. Fluorimetry had the advantage that the results could be read within 1–5h and the reproducibility was superior to the other methods.     

Rapid analysis of polymer homologues and additives with SFE/SFC-MS coupling

A rapid and easy analysis method for polymers is presented. The method involves sample preparation by SFE, separation of the extracted compounds by SFC and simultaneous quantitative detection by FID, as well as identification of unknowns by MS. The applications illustrate how structural research work and routine polymer analysis can be done with this time saving method.     

Effect of irradiation and packaging type on sensory quality of chill-stored turkey breast fillets

A panel of experienced assessors was used to test the effect of irradiation at 2.5kGy (250krad) on the sensory quality of 1-day and 21-day-old turkey breast fillets stored at 1°C in either oxygen-permeable polythene or an oxygen-impermeable barrier film. In the application of Free Choice Profiling and Generalized Procrustes Analysis, assessors used their own terms to describe the appearance, odour and flavour of the samples.
The irradiated samples showed negligible growth of micro-organisms across 21 days of storage. Radiation treatment resulted in an intense pink colour in the raw and cooked samples, which was maintained during storage in oxygen-impermeable film, but decreased in samples exposed to oxygen during storage. Radiation also produced a set of unpleasant raw odour notes variously described as sour, rancid, mature, bad meat or putrid in the samples stored in oxygen-impermeable film. These notes were unlike the sulphurous notes previously associated with protein denaturation in irradiated chicken and were apparently distinct from the odour notes that developed in corresponding non-irradiated samples.     

集成电路密封用高纯邻甲酚醛环氧树脂的制备

ECN树脂中的可水解氯含量是决定树脂质量的—个重要指标。本文研究了反应温度、ECH/OCN配比、反应时间和碱用量等因素的影响,取得了规律性的认识,并获得最佳配方,制备出高纯度的ECN树脂系列产品。     

基于嵌入式系统开发平台的设计与实现

本文首先介绍了EVB2107评估板、评估板的系统开发工具和集成开发环境,然后对扩展板上各模块都从原理入手结合该模块要实现的功能说明其设计思想和实现原理,并就各模块驱动程序的编程思想及流程进行了论述。     

临安市城乡供水一体化的供水能力确定

文章采用分项定额法，单位人口综合用水量指标法和基准年法分别计算，确定城乡供水的供水能力。     

整体经济效益最优控制方法

本文提出了整体经济效益最优的动静态耦合的优化控制一体化方法，它在传统的两级优化控制结构中加入一层协调级，能有效地克服以往优化级撇开控制能量代价不计的缺陷。特别是一体化系统通过调级从控制层向优化层引入反馈，使得由于过程自身动态因素对操作点的制约，能有效地通知到优化层，避免了优化的盲目性和片面性。各个层次之间通过公用数据库交换数据，使它们的决策与目前生产过程的实际情况密切联系。     

Integrated solid-state nanopore devices for third generation DNA sequencing

Third generation DNA sequencing relies on monitoring the ionic current blockage during the DNA molecule’s threading through a nanoscale pore.It is still really tough to attain the single base discrimination on a DNA strand by merely analyzing the ionic current due to speedy DNA translocation and low spatial resolution.More integrated configurations are pursued to present versatile comparative dissimilarities of the four bases by enhancing the spatial resolution within a DNA molecule translocation event,such as transverse tunneling current,local potential change,and capacitance oriented voltage resonance.In this mini review,the insight is provided into the status quo on several functionalized techniques and methodologies for DNA sequencing and furthermore concluding remark and outlook are presented.     

超高层综合楼消防自控设计探析

讨论了超高层综合楼消防自控设计的必要性、可能性和具体的设计方法.