Kinetic analysis for effect of initial substrate concentration on growth and secondary metabolite production in cultures of Nicotiana tabacum

Suspension cultures of tobacco cells were studied using airlift and rotary-drum bioreactors. The effect of initial concentrations of a major substrate, sucrose, on the growth and production of a secondary metabolite, phenolic compounds, was investigated. The dry weights and total concentrations of the phenolic compounds increased with the initial sucrose concentration in both bioreactors. Both bioreactors were found to have the same tendency for the effect of initial sucrose concentration. The structured model, presented previously was modified by considering that sucrose was hydrolyzed to glucose and fructose by an enzymatic reaction. The previous and the new models were applied to the above two sets of experimental data obtained with two bioreactors, independently. The hydrolysis of sucrose was elucidated to contribute slightly to the overall kinetics of growth and secondary metabolite production in these cultures. Furthermore, the levels of shear damage in each bioreactor were quantitatively compared based on the death rate constant, k_i, which is one of the model constants.     

中国观赏石文化与收藏

以推动人类文明进步发展的观念为先导,以详实的地质学、岩石学、石文化史料为切入点,介绍了我国观赏石文化的起源、发展及现状;阐述了赏石审美的本质、形式、石体标准、传统性、现代性等理论要点及其以小见大、以丑为美的审美理念。对赏石修身养性、以石陶情及赏石文化与道教情结的学术价值进行了评述;对从古至今的中华文化名人为赏石理论的创立、鉴赏、收藏和发展作出的不可磨灭的历史贡献进行了评价。     

新型城镇化下欠发达地区传统文化保护研究——以张家口市段家堡村为例

新型城镇化背景下欠发达地区传统文化保护的关键取决于其能否在保护文化资本和环境资本的基础上,实现经济资本的提升、文化价值观念及生活方式的转变.通过对张家口市下花园区段家堡村传统文化的调查研究,提出转变观念,主动保护、挖掘和丰富欠发达地区传统文化特色,提升旅游开发优势,带动地区经济发展,实现新型城镇化的同时更好地保护传统文化.     

民俗文化语境下的高校艺术设计教育

尽管当下中国高等艺术设计教育呈现出前所未有的迅猛发展态势,但在学科建设和教育实施方面也显示出一定的不足和问题,在诸多直接的原因之外,本文立足于深层文化思考,对中国视觉民俗文化主体之一的民间美术所蕴涵的文化意义作了进一步的透析,以期寻绎出在宏阔的民俗文化生态中我国高校艺术设计教学改革的策略与教育发展取向。     

浅谈在艺术设计教育中加强中国传统文化学习的必要性

近年来,正当许多发达国家努力发展本土文化的时候,我国在很多方面特别是艺术设计教育领域却忽视了具有5000年历史的中国传统文化。传统文化在现代设计教育中缺失的问题,表面上是教育体系、教育方法的问题,实际是教育理念的问题。面对西方文化巨大冲击,完善我国现代设计教育体系,确保民族文化根脉的活力,是我们必须面对、也必须尽快解决的问题。     

基于传统文化艺术资源整合基础上的山东区域特色动漫设计研究

山东区域的传统文化艺术资源主要包括:民间传说、寓言故事、文学作品、文物古迹、传统艺术等内容。     

Development of high throughput microfluidic cell culture chip for perfusion 3-dimensional cell culture-based chemosensitivity assay

This study reports a microfluidic cell culture chip encompassing 36 microbioreactors for high throughput perfusion 3-dimensional (3D) cell culture-based chemosensitivity assays. Its advantages include the capability for multiplexed medium delivery, and the function for both efficient and high throughput micro-scale 3D culture construct preparation and loading. The results showed that the proposed medium pumping mechanism was able to provide a uniform pumping rates ranging from 1.2 to 3.9 μl h⁻¹. In addition, the simple cell/hydrogel loading scheme has been proven to be able to carry out 3D cell culture construct preparation and loading precisely and efficiently. Furthermore, a chemosensitivity assay was successfully demonstrated using the proposed cell culture chip. The results obtained were also compared with the same evaluation based on a conventional 2D monolayer cell culture. It can be concluded that the choice of cell culture format can result in different chemosensitivity evaluation results. Overall, because of the nature of miniaturized perfusion 3D cell culture, the cell culture chip not only can provide stable, well-defined and more biologically relevant culture environments, but it also features low consumption of research resources. All these traits are found particularly useful for high-precision and high-throughput 3D cell culture-based assays.     

试论女性主义文化在产品设计中的符号化演绎

设计总是与文化的多样性有着错综复杂的关系,女性主义作为一种主体意识强烈、情感导向明确的文化,其在设计中的融入,是设计发展过程中的必然。本文从三个案例出发,尝试解读女性主义文化的部分内容在产品设计中的符号化演绎。     

经典传说的新语境分析:以牛郎织女传说为例

牛郎织女传说作为我国四大传说之一,在历史传承中发生了文化变迁,其影响日益式微。在当代语境下,后现代主义思潮的推动,新科技手段的应用,为牛郎织女传说的传承提供了新的机遇。本文拟就对牛郎织女历史语境及当代语境作出分析,同时对牛郎织女未来保护与传承进行构想与展望,思考牛郎织女传说的发展辙迹。