Effect of 5-fluorouracil and UFT on experimental liver metastasis model of colorectal cancer using mouse colon 26 cells

Effects of 5-fluorouracil (5-FU) and UFT on an experimental liver metastasis model were compared at equi-effective dosage levels against subcutaneous tumor of mouse colon 26. 5-FU at the dosage level of 40 mg/kg suppressed the subcutaneous tumor growth by 70.0% and 45.0% on day 13 and day 18, respectively, and UFT at 20 mg/kg provided almost equal suppression (63.0% and 48.0%). In the liver metastasis model, 5-FU at 40 mg/kg showed more potent prevention of the formation of metastatic foci (94.9%) than did UFT (60.4%) at 20 mg/kg. 5-FU at 40 mg/kg produced a much higher peak serum level of 5-FU than did UFT at 20 mg/kg and also showed a much higher AUC (area under the curve) level in the portal blood. These results suggest that oral administration of 5-FU might be useful in prevention of liver metastasis of colorectal cancer.     

Space charge behavior in polymer film based on increment of capacitance under AC high field

Polymer materials have excellent dielectric and insulation properties; however, those properties in AC high field region have not been known well. Recently we established an evaluation method of high‐field AC dissipation current waveform of polymer materials 1 . AC dissipation current waveforms of polyethylene and polypropylene films show nonlinear distortion in AC high‐field region. This nonlinearity was thought to be related to the behavior of AC space charge formation in the sample near electrodes. The properties of space charge formed under AC high field at power frequency seem to differ from those formed under DC high field. The measurement of AC space charge distribution is not so easy due to the resolution limit of the space charge measurement. We studied the dielectric properties of biaxially oriented polypropylene (BOPP) film under AC high field up to 120 °C. It was found that tan δ, AC dissipation current (Ixr), and unbalanced component of capacitive current (ΔIxc) increased when the temperature became higher. In particular, ΔIxc increased above some threshold field and was considered to be due to the AC space charge formation. This AC space charge layer near electrode is thought to be formed due to carrier injection under AC high‐field application. Usually, the carrier mobility becomes smaller on lowering the temperature. Most of the carriers injected from the electrode are trapped near the electrode in the sample film. But in the high‐temperature region, the carrier mobility becomes larger and the carrier injection starts to increase from lower field. Many more carriers are injected from the electrode. It is thought that some of the injected carriers are trapped inside the sample film; the others go through the sample to the opposite side. © 2002 Wiley Periodicals, Inc. Electr Eng Jpn, 141(2): 8–16, 2002; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.10018     

Tan δ of ethylene-propylene rubber in cryogenic temperature region–effects of additives and sample thickness

The authors have been developing extruded polymer insulated superconducting power cables. Dielectric loss in electrical insulation cannot be ignored in superconducting cables since conductor loss in the cables is minimal. Studies so far show that ethylene-propylene rubber (EPR) is suitable as an electrical insulating material in the extruded polymer insulated superconducting cable design because it demonstrates excellent mechanical and relatively good electrical qualities at a cryogenic temperature. Widely used EPR includes some kinds of additives; however, their effect on tan δ of EPR at cryogenic temperature remains unknown. The effect of additives such as crosslinking agent and fillers on tan δ of EPR was examined at temperatures of between 4.2 K and 300 K. Thickness dependence of tan δ was also measured using EPR films of different thickness and an extruded EPR insulated cable sample. The results show that additives increase tan δ of relatively thin films of EPR even in the cryogenic temperature region; however, they do not have an intolerable dielectric loss in comparison with conductor loss and heat inflow of the superconducting cable. The remedy to tan δ increase due to the polymer contraction relative to shielding wires has been established.     

Infection with hepatitis G virus and its strain variant, the GB agent (GBV-C), among blood donors in Japan

Much of the morbidity and mortality seen in cystic fibrosis (CF) is related to chronic infection of the respiratory tract with Pseudomonas aeruginosa. Some studies have attributed the strong relationship between CF and Pseudomonas colonization to the presence of increased numbers of specific cell-surface receptors, although other work suggests that this relates to the presence of mucus. Several groups are now assessing the use of gene transfer as a novel form of treatment for CF. We have examined whether P. aeruginosa binding to freshly obtained CF respiratory epithelial cells is increased, and have studied the effects of transfer of the CF transmembrane conductance regulator (CFTR) gene on this attachment. Binding of P. aeruginosa to noncultured nasal epithelial cells from both CF patients (n = 31) and healthy controls (n = 15) was studied with scanning electron microscopy. Binding was also assessed for CF cells following transfection with CFTR/liposome complexes. Epifluorescence microscopy was used to assess the effects of gene transfer on chloride fluxes. Adherence of P. aeruginosa directly to the cell surface of CF airway epithelium was significantly (P < 0.001) increased over that in non-CF controls. Liposome-mediated CFTR gene transfer resulted in a significant (P < 0.01) reduction in the numbers of bacteria bound to ciliated epithelial cells. Fluorescence microscopy confirmed correction of the basic chloride defect. Thus, in CF, the absence of normal CFTR results in increased binding of P. aeruginosa to respiratory epithelial cells. This abnormality can be corrected in vitro by restoration of CFTR function. This has important implications both for the pathogenesis of CF and for the future application and assessment of gene therapy for this disease.     

Development of extruded ethylene propylene rubber insulated superconducting cable

A superconducting power cable is one of the promising ways of underground transmission of huge electric power in the future. The authors have long proposed the idea of the extruded polymer insulation for superconducting cables. The prominent features of the design are to exploit the excellent electrical properties of polymer in the cryogenic temperatures and to separate the helium coolant from the electrical insulation. Although the extruded cross-linked polyethylene cable has proved ability at the liquid nitrogen temperature, the cable insulation cracked due to mechanical stress during cooling to the liquid helium temperature. To overcome this problem, ethylene propylene rubber (EPR) was selected as a new insulating material considering the good results of mechanical and electrical tests of EPR samples at cryogenic temperatures. An extruded EPR insulated superconducting cable 15 m in length was fabricated and a cooling test down to the liquid helium temperature and a voltage test at the liquid helium temperature were carried out with fair success. This is a breakthrough in terms of the electrical insulation design of cryogenic cables.     

Properties of electrical insulating materials at cryogenictemperatures: A literature review

Many insulating materials and systems for superconducting electrical equipment have been developed in response to the severe demands of safe usage, such as in cryogenic temperature, high mechanical stresses, high radiation, etc. They have been designed to fulfill very extreme service conditions, either at liquid helium or liquid nitrogen temperatures. Whatever the future industrial development, there is already abundant knowledge about insulating materials at cryogenic temperatures, and the feasibility has been proven. Many laboratories in the world continue research to increase this knowledge and to fill in the remaining gaps     

High-field dielectric properties of polyethylene in the high-temperature region

Nonpolar polymers such as polyethylene or polypropylene are widely used as insulation materials, often under conditions involving strong electric fields and high temperatures. Under these conditions, the conduction loss due to DC-like carrier transport may give rise to a high-field AC dissipation factor (tan δ). This paper describes the high-field dielectric properties of polyethylene films at frequencies from 50 to 400 Hz and temperatures from room temperature to 100°C, as measured with a new type electrode design developed by the authors. In the high-temperature region, the AC dissipation current waveform at 50 Hz was also measured. At room temperature, the electric-field dependence of tan δ is almost unaffected by the frequency of the applied field. But at high temperatures and strong electric fields, tan δ tends to have a large field dependence and to be almost inversely proportional to the frequency. Thus the AC conductivity becomes independent of the frequency of the applied field in the region of strong fields and high temperatures. A theoretical analysis shows that the high-field dielectric loss is governed by two processes, namely, the dielectric relaxation loss inherent to the AC field and the DC-like carrier transport loss that becomes prominent in the high-temperature region, where AC dissipation current waveform becomes significantly nonsinusoidal.