Quantitative determination of complex carbohydrates in bovine milk and in milk-based infant formulas.

Quantitative determination of all structural families of complex carbohydrate micronutrients was performed on bovine milk samples, milk-based infant formulas, and whey-based manufacturing raw materials. Differences found between formulas depended mainly on their whey: casein ratios. A solvent separation procedure was required for quantitative estimation of the gangliosides and neutral glycolipids within the fat fraction. All infant formulas except one contained slightly more gangliosides than bovine milk. Complex carbohydrates were consistently higher in the nonfat fraction. By gel permeation chromatography, an oligosaccharide subfraction was separated from a glycopeptide one. Oligosaccharide content of infant formulas increased as a function of the whey:casein ratio, and glycopeptides were found only in formulas made with whey components. Neuraminic acids from infant formulas were associated primarily with the glycoprotein fraction, except in hydrolysate-based preparations in which "precipitable" glycoproteins were converted into "soluble" glycopeptides by trypsin treatment. Because whey-based raw materials are very rich in all bovine milk glycoconjugates and oligosaccharides their increased use will result in high contents of these micronutrients in modern formulas.     

The P1605 and P1606 working group report

This article presents a history to date for P1605, a guide for comparison of existing installation requirements for electrical distribution and utilization equipment in North America and Europe, and P1606, a guide for comparison of existing electrical safety standards for employee workplace in North America and Europe. The comparison will address requirements contained in North American standards (OHSA, NFPA70E) and European standards EN 50110 highlighting similarities and differences in these standards.     

Proper complex random processes with applications to informationtheory

The covariance of complex random variables and processes, when defined consistently with the corresponding notion for real random variables, is shown to be determined by the usual complex covariance together with a quantity called the pseudo-covariance. A characterization of uncorrelatedness and wide-sense stationarity in terms of covariance and pseudo-covariance is given. Complex random variables and processes with a vanishing pseudo-covariance are called proper. It is shown that properness is preserved under affine transformations and that the complex-multivariate Gaussian density assumes a natural form only for proper random variables. The maximum-entropy theorem is generalized to the complex-multivariate case. The differential entropy of a complex random vector with a fixed correlation matrix is shown to be maximum if and only if the random vector is proper, Gaussian, and zero-mean. The notion of circular stationarity is introduced. For the class of proper complex random processes, a discrete Fourier transform correspondence is derived relating circular stationarity in the time domain to uncorrelatedness in the frequency domain. An application of the theory is presented     

Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions

Thirteen human bifidobacterial strains were tested for their abilities to adhere to human enterocyte-like Caco-2 cells in culture. The adhering strains were also tested for binding to the mucus produced by the human mucus-secreting HT29-MTX cell line in culture. A high level of calcium-independent adherence was observed for Bifidobacterium breve 4, for Bifidobacterium infantis 1, and for three fresh human isolates from adults. As observed by scanning electron microscopy, adhesion occurs to the apical brush border of the enterocytic Caco-2 cells and to the mucus secreted by the HT29-MTX mucus-secreting cells. The bacteria interacted with the well-defined apical microvilli of Caco-2 cells without cell damage. The adhesion to Caco-2 cells of bifidobacteria did not require calcium and was mediated by a proteinaceous adhesion-promoting factor which was present both in the bacterial whole cells and in the spent supernatant of bifidobacterium culture. This adhesion-promoting factor appeared species specific, as are the adhesion-promoting factors of lactobacilli. We investigated the inhibitory effect of adhering human bifidobacterial strains against intestinal cell monolayer colonization by a variety of diarrheagenic bacteria. B. breve 4, B. infantis 1, and fresh human isolates were shown to inhibit cell association of enterotoxigenic, enteropathogenic, diffusely adhering Escherichia coli and Salmonella typhimurium strains to enterocytic Caco-2 cells in a concentration-dependent manner. Moreover, B. breve 4 and B. infantis 1 strains inhibited, dose dependently, Caco-2 cell invasion by enteropathogenic E. coli, Yersinia pseudotuberculosis, and S. typhimurium strains.