Transforming Perceived Vocal Effort and Breathiness Using Adaptive Pre-Emphasis Linear Prediction

This paper presents a technique to transform high-effort voices into breathy voices using adaptive pre-emphasis linear prediction (APLP). The primary benefit of this technique is that it estimates a spectral emphasis filter that can be used to manipulate the perceived vocal effort. The other benefit of APLP is that it estimates a formant filter that is more consistent across varying voice qualities. This paper describes how constant pre-emphasis linear prediction (LP) estimates a voice source with a constant spectral envelope even though the spectral envelope of the true voice source varies over time. A listening experiment demonstrates how differences in vocal effort and breathiness are audible in the formant filter estimated by constant pre-emphasis LP. APLP is presented as a technique to estimate a spectral emphasis filter that captures the combined influence of the glottal source and the vocal tract upon the spectral envelope of the voice. A final listening experiment demonstrates how APLP can be used to effectively transform high-effort voices into breathy voices. The techniques presented here are relevant to researchers in voice conversion, voice quality, singing, and emotion.     

Evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus

Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.     

Towards a verifiable real-time, autonomic, fault mitigation framework for large scale real-time systems

Designing autonomic fault responses is difficult, particularly in large-scale systems, as there is no single ‘perfect’ fault mitigation response to a given failure. The design of appropriate mitigation actions depend upon the goals and state of the application and environment. Strict time deadlines in real-time systems further exacerbate this problem. Any autonomic behavior in such systems must not only be functionally correct but should also conform to properties of liveness, safety and bounded time responsiveness. This paper details a real-time fault-tolerant framework, which uses a reflex and healing architecture to provide fault mitigation capabilities for large-scale real-time systems. At the heart of this architecture is a real-time reflex engine, which has a state-based failure management logic that can respond to both event- and time-based triggers. We also present a semantic domain for verifying properties of systems, which use this framework of real-time reflex engines. Lastly, a case study, which examines the details of such an approach, is presented.     

Skin surface microflora of the saddle-back tamarin monkey,Saguinus fuscicollis

The resident skin surface microflora of 12 male and three femaleSaguinus fuscicollis was studied. The suprapubic-circumgenital gland surface and the perirectal area were most heavily colonized (10⁶–10⁸ colony forming units/cm²), but high numbers of organisms were also present on the abdomen, the sternal gland surface, and palms and plantar surfaces. Bacteria were also recovered from hair clippings as well as from shaven skin surface, although at much lower densities (10²–10⁴ colony forming units/cm²). Coagulase negative staphylococci, gram-negative bacteria,Streptococcus species and coryneform bacteria were most dominant.Staphylococcus aureus and species ofBacillus were also present. Fungi, yeast, and dermatophytes were infrequently recovered or completely absent. The microbial flora of these tamarins appears to be closely associated with the secretions of the circumgenital scent gland and would therefore be ideally situated to participate in the generation or modification of chemical signals.     

Advanced mercury removal from gold leachate solutions prior to gold and silver extraction: a field study from an active gold mine in Peru

Mercury contamination in the Gold-Cyanide Process (GCP) is a serious health and environmental problem. Following the heap leaching of gold and silver ores with NaCN solutions, portions of the mercury-cyano complexes often adhere to the activated carbon (AC) used to extract the gold. During the electrowinning and retorting steps, mercury can be (and often is) emitted to the air as a vapor. This poses a severe health hazard to plant workers and the local environment. Additional concerns relate to the safety of workers when handling the mercury-laden AC. Currently, mercury treatment from the heap leach solution is nonexistent. This is due to the fact that chelating ligands which can effectively work under the adverse pH conditions (as present in the heap leachate solutions) do not exist. In an effort to economically and effectively treat the leachate solution prior to passing over the AC, a dipotassium salt of 1,3-benzenediamidoethanethiol (BDET2-) has been developed to irreversibly bind and precipitate the mercury. The ligand has proven to be highly effective by selectively reducing mercury levels from average initial concentrations of 34.5 ppm (parts per million) to 0.014 ppm within 10 min and to 0.008 ppm within 15 min. X-ray powder diffraction (XRD), proton nuclear magnetic resonance (1H NMR), Raman, and infrared (IR) spectroscopy demonstrate the formation of a mercury-ligand compound, which remains insoluble over pH ranges of 0.0-14.0. Leachate samples from an active gold mine in Peru have been analyzed using cold vapor atomic fluorescence (CVAF) and inductively coupled plasma optical emission spectroscopy (ICP-OES) for metal concentrations before and after treatment with the BDET2- ligand.     

Experimental measurements of orientation and rotation of dense 3D packings of spheres

Many recent advances in the study of granular media have stemmed from the improved capability to image and track individual grains in two and three dimensions. While two-dimensional systems readily yield both translational and rotational motion, a challenge in three-dimensional experiments is the tracking of rotational motion of isotropic particles. We propose an extension of the refractive index matched scanning technique as a method of measuring individual particle rotation. Initial measurements indicate that shear-driven rotational motion may stem from gear-like motion within the shear zone.     

FASTA barcodes: a simple method for the identification of yeast ORF deletions

A consortium of yeast geneticists have created -6000 individual ORF deletions, representing > 96% of the currently verified or predicted ORFs in S. cerevisiae. Importantly, molecular barcodes (each a unique 20 bp sequence termed either Uptag or Downtag) were used as identifiers for every ORF deletion. Microarray analyses of pooled yeast deletions has been used to identify thousands of genes involved in general fitness, haploinsufficiency, drug resistance and DNA damage repair. However, application of this powerful technology requires considerable expense, expertise and specialized equipment. While standard PCR techniques and specifically designed PCR primers can be used to confirm that a given ORF is in fact deleted, this procedure cannot be used to identify unknown deletions. In theory, every ORF deletion could be determined by barcode sequencing. However, neither a consolidated barcode database nor a reliable search engine is currently available for this purpose. To address this need, we have adapted a FASTA sequence program that utilizes the unique barcode database to allow users to identify individual ORF deletions, based upon simple sequencing reactions of PCR amplifications of either Uptag or Downtag barcodes. In silico and practical testing of this application reveals that it is an inexpensive, reliable and reproducible method for rapidly identifying unknown deletions. This approach allows laboratories to conduct small- or large-scale genetic screens with pooled yeast deletion strains and identify or verify any ORF deletion without the need for microarray technology.     

Explosive strengthening of a Cu-Be alloy

The effects of shock loading on the mechanical properties and microstructure of a precipitation hardening copper 1.91 wt pct beryllium alloy were studied. Sheet samples suitable for tensile testing were explosively loaded to pressures of 10, 20, 30, 40, and 50 GPa. Subsequent tensile tests were performed on samples in the as-shocked and shocked plus aged conditions. Their properties are compared to those of material deformed by cold rolling and aged after cold rolling. The mechanical strength in the as-shocked condition increases linearly with pressure. The strength of the shocked plus aged material also increases with shock pressure but at a slower rate. Unaged material shocked to 50 GPa has a higher yield strength than material cold rolled 37 pct. However, the cold rolled material responds better to aging. In the aged condition, 37 pct cold rolled material is stronger than the material shocked to 50 GPa. Transmission electron microscopy reveals the onset of deformation twinning between 10 and 20 GPa. The volume fraction of twins and the dislocation density both increase with increasing shock pressure. The results indicate that deformation twinning has little significant strengthening effect in this system and that yield strength increases can be accounted for on the basis of increasing dislocation density.