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1.
The UvrA and UvrB proteins form part of the UvrABc endonuclease, which is responsible for nucleotide excision repair in Escherichia coli. Using a mobility shift gel assay we have studied the binding of UvrA dimer, UvrB monomer and UvA(2)B trimer complexes with 40, 50 and 136 bp (32)P-end-labelled DNA fragments adducted with aflatoxin B(1). UvrA was shown to re-associate with adduct specific UvrB: DNA complexes, a phenomenon which could be reversed by the addition of 500 mM potassium chloride or anti-UvrA anti-sera. Re-association was shown to be UvrA concentration dependent. Re-association of UvrA(2)B to the UvrB:DNA complex was not seen. We have also shown that the UvrB:DNA complex, in the case of aflatoxin B(1), is extremely stable with a half-life excess of 400 min and that fragment termini are not a specific substrate for UvrA binding. 相似文献
2.
Martti Heinonen Miltiadis Anagnostou Stephanie Bell Mark Stevens Robert Benyon Reidun Anita Bergerud Jovan Bojkovski Rien Bosma Jan Nielsen Norbert B?se Plunkett Cromwell Aliye Kartal Dogan Seda Aytekin Ali Uytun Vito Fernicola Krzysztof Flakiewicz Bertrand Blanquart Domen Hudoklin Per Jacobson Anders Kentved Isabel Lóio George Mamontov Alexandra Masarykova Helmut Mitter Regina Mnguni Jan Otych Anton Steiner Nagyné Szilágyi Zsófia Davor Zvizdic 《International Journal of Thermophysics》2012,33(8-9):1422-1437
In the field of humidity quantities, the first CIPM key comparison, CCT-K6 is at its end. The corresponding European regional key comparison, EUROMET.T-K6, was completed in early 2008, about 4?years after the starting initial measurements in the project. In total, 24 NMIs from different countries took part in the comparison. This number includes 22 EURAMET countries, and Russia and South Africa. The comparison covered the dew-point temperature range from ?50?°C to +20?°C. It was carried out in three parallel loops, each with two chilled mirror hygrometers as transfer standards in each loop. The comparison scheme was designed to ensure high quality results with evenly spread workload for the participants. It is shown that the standard uncertainty due to the long-term instability was smaller than 0.008?°C in all loops. The standard uncertainties due to links between the loops were found to be smaller than 0.025?°C at ?50?°C and 0.010?°C elsewhere. Conclusions on the equivalence of the dew-point temperature standards are drawn on the basis of calculated bilateral degrees of equivalence and deviations from the EURAMET comparison reference values (ERV). Taking into account 16 different primary dew-point realizations and 8 secondary realizations, the results demonstrate the equivalence of a large number of laboratories at an uncertainty level that is better than achieved in other multilateral comparisons so far in the humidity field. 相似文献
3.
Established methods for the chemical analysis of dissolved metal are concisely reviewed. In each case, the principles of a given technique are considered and its relative merits are outlined. 相似文献
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5.
Plunkett Allan B. D'Atre John D. Lipo Thomas A. 《Industry Applications, IEEE Transactions on》1979,(4):430-437
Conventional methods of controlling an induction motor utilize regulation of stator current and motor slip frequency in order to maintain system stability. This control strategy requires a shaft speed feedback and fast-response current regulation. An alternative method of controlling an induction motor is presented which achieves the necessary system stabilization by controlling only the motor frequency. The control inherently regulates the motor torque angle by properly adjusting the phase of the converter firing signals. By synchronizing the inverter firing pulses to the motor back electromotive force (EMF) possible adverse inverter operating modes are avoided. The concept of synchronous control eliminates the preprogrammed functional relations previously required and allows the control to adapt to any desired motor flux level. Any desired outer regulating loop can be incorporated to form a fast-response wide-range ac drive system. 相似文献
6.
J. Stander J. Plunkett W. Michalson J. McNeill R. Ludwig 《Journal of Nondestructive Evaluation》1997,16(4):205-214
This paper describes a new instrumentation approach to the nondestructive testing of green-state powdered metallurgy components. These samples are likely to generate surface-breaking and subsurface defects prior to sintering. Exploiting the principles of electric resistivity or potential drop measurements in solids, a system is configured which is capable of recording surface voltage distributions due to impressed current inputs. At the heart of this novel testing procedure is a multiple-pin sensor which allows for flexible measurement conditions in order to cover wide surface areas. Practical tests with production samples compare well with both analytical and numerical modeling techniques in predicting surface voltage distributions. Furthermore, initial studies of surface-breaking flaws exhibit excellent defect detectability. 相似文献
7.
Lubica Macakova Gleb E. YakubovMark A. Plunkett Jason R. Stokes 《Tribology International》2011,44(9):956-962
We have studied tribological properties of pre-adsorbed salivary films formed in vitro on compliant hydrophobic surfaces. The adsorbed salivary film significantly decreases boundary friction under physiological ionic strength, which is related to a hydrophilic character of the adsorbed film and its structure. Decrease in the ionic strength below physiological conditions affects film’s structure, but it does not significantly affect boundary lubrication at low loads. Applications of high loads led to a gradual loss of lubrication due to shear-induced wear of the films. The wear became more extensive as the ionic strength of the solvent was lowered below physiological conditions. 相似文献
8.
Which objects and animals are children willing to accept as referents for words they know? To answer this question, the authors assessed early word comprehension using the preferential looking task. Children were shown 2 stimuli side by side (a target and a distractor) and heard the target stimulus named. The target stimulus was either a typical or an atypical exemplar of the named category. It was predicted that children first connect typical examples with the target name and broaden the extension of the name as they get older to include less typical examples. Experiment 1 shows that when targets are named, 12-month-olds display an increase in target looking for typical but not atypical targets whereas 24-month-olds display an increase for both. Experiment 2 shows that 18-month-olds display a pattern similar to that of 24-month-olds. Implications for the early development of word comprehension are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
9.
We report a case of a depressed patient who received a full course of electroconvulsive therapy (ECT) 2 weeks post four-vessel coronary artery bypass graft surgery (CABG). ECT was well tolerated, and a full remission was induced. In spite of the lack of information in the literature concerning guidelines for administering ECT to a patient with recent postoperative status, we were encouraged by this patient's response. This case suggests that ECT may be considered a viable treatment option for refractory depression associated with severe medical comorbidity and recent post-operative status such as in CABG. 相似文献
10.