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1.
Molecular cloning of a canine metallothionein cDNA 总被引:1,自引:0,他引:1
A canine metallothionein cDNA obtained from the liver of a cadmium-treated beagle was cloned and sequenced. Asn at position 4 conserved among all mammalian metallothionein-1 and metallothionein-2 is replaced by Asp in the canine metallothionein cDNA clone. Because the acidic amino acid doesn't exist at either position 10 or 11 in the deduced amino acid sequence, it is supposed that this cDNA is derived from canine metallothionein-1 mRNA. Northern blot analysis using the cDNA as a probe revealed the induction of the canine metallothionein mRNA expression in the liver and kidney of a cadmium-treated beagle. Thus, the canine metallothionein cDNA obtained in the present study should provide an useful tool for the molecular investigation of metallothionein in dog. 相似文献
2.
A Yoshimura Y Yamano A Gemma K Yoshimori K Hayashihara Y Taniguchi K Uematsu M Shibuya S Kudoh H Niitani 《Canadian Metallurgical Quarterly》1993,20(4):503-508
We conducted an early phase II trial in advanced non-small cell lung cancer (NSCLC) to evaluate response efficacy of a combination of Cisplatin (CDDP) and Carboplatin (CBDCA). The twenty-six patients in the study had had no previous treatment. They received a sequential administration of 300 mg/m2 CBDCA and 80 mg/m2 CDDP with approximately 3,500 ml of hydration on day 1 every 4 weeks. All patients were evaluable for response and toxicity. Ten (38.5%) of all assessable patients achieved a partial response (95% confidence interval, 19.8-57.2%). Response rates for patients with stage III A, III B and IV- disease were 40.0 (2/5), 70.0 (7/10) and 9.1% (1/11), respectively. Response rates for patients with squamous cell carcinoma, adenocarcinoma and large cell carcinoma were 35.7 (5/14), 45.5 (5/11) and 0.0% (0/1), respectively. The median survival time (MST) of all patients was 11 months. The MST for patients with stage III disease was 14 months; for those with stage IV disease it was 7 months. The MST for responding patients was 15 months and for not responding patients 5 months. Major toxicities were hematologic and gastrointestinal, and the dose-limiting factor was thrombocytopenia. This combination chemotherapy was effective against NSCLC with tolerable toxicities. Further trials are warranted to determine the efficacy of the combination chemotherapy. 相似文献
3.
The total volume change included gel and surrounded water with the swelling of five types of speherical cross-linked dextran (Sephadex) that are either nonionic (G) or possess one of four different ionic groups in the same molecular skeleton: CM: sodium carboxymethyl, SP: sodium sulphopropyl, DEAE: diethylaminoethyl chloride, and QAE: diethyl-(2-hydroxypropyl) aminoethyl chloride. All have been studied by dilatometry. The total volume decreased with the swelling of all Sephadexes. The maximum changes of total volume with the swelling in water were all negatives of CM, G, SP, (DEAE, QAE), which decrease in that order. These values did not depend on the concentration of sodium chloride and were closely related to the maximum heats of swelling. Total volume changes occurred by the dissociation of the ionic group of dextran derivatives that subtracted the total volume change due to hydration of Sephadex skeleton from the total volume change: ?21.6 ± 3.1 μL mmol?1 for CM, ?2.8 ± 8.0 μL mmol?1 for SP, +13.2 ± 4.4 μL mmol?1 for DEAE, and +15.4 ± 4.6 μL mmol?1 for QAE. These values are assumed to be reflected in the quantities of ions-water interaction of Sephadexes. © 1994 John Wiley & Sons, Inc. 相似文献
4.
Suharyanto Yamano Y. Kobayashi S. Michizono S. Saito Y. 《Dielectrics and Electrical Insulation, IEEE Transactions on》2006,13(1):72-78
The breakdown of alumina rf windows is mostly caused by multipactor, as well as by material defects and contamination. Since multipator induces localized surface heating, leading to surface melting, it is necessary to observe secondary electron emission (SEE) coefficients of alumina ceramics under high temperature conditions. The SEE coefficients of commercial alumina ceramics and sapphire were measured by a scanning electron microscopy (SEM) with a single short-pulsed electron beam (100 pA, 1 ms) at room temperature and at 650degC. Additive materials used for sintering alumina, such as SiO 2 and MgO, were also investigated. Surface charging evaluations have also become important because the accumulated charges are discharged at the threshold Held, resulting in surface discharge. The surface charging evaluations were carried out by multi-pulse measurements with the injection of successive pulses on the sample. As a result, reductions in the SEE coefficients with temperature were confirmed, except for sapphire. The multi-pulse measurement results indicated that surface charging of the sapphire was higher than that of other samples. This may be one of the factors that causes sapphire not to be durable for rf window applications, compared with alumina ceramics. Although there are few exceptions, it was found that the SEE coefficients of alumina ceramics increased with the purity and the average grain size 相似文献
5.
Previous studies on polyamide 4, excellent properties, functionalities, and biodegradation in natural condition have been shown. In this study, three-branched (star-shaped) copolyamides constituted of polyamide 4 and polyamide 6 constitutional unit were synthesized by anionic ring-opening copolymerization of 2-pyrrolidone with ε-caprolactam. The thermal and mechanical properties and the biodegradability of the obtained copolyamides have been systematically investigated. The weight-average molecular weight of the copolyamides was as high as tens of thousands (Mw 10–80 × 103 g/mol). The composition of the copolyamides was approximately in accord with the monomer feed ratio, thereby being controllable. The thermal and mechanical properties changed readily as the composition was varied (Tm 146–266°C, ΔHm 10–70 J/g, Td 278–369°C, tensile strength 28–64 MPa, elongation at break 80–750%). The copolyamide having 2-pyrrolidone unit of 96–51 mol% exhibited biodegradability by an activated sludge. The biodegradation of the copolyamide proceeded uniformly without disproportion in constitutional unit. 相似文献
6.
The purpose of this study is to decrease PD at a triple junction by addition of a dye to an insulating material. PD is produced on a test specimen subjected to ac voltage under a sphere-plane electrode configuration. The specimen is composed of two layers: a PMMA film with additive, which is 100 μm thick; the lower layer a PMMA board 3 mm thick. The film with the additive was prepared by the solution-grown casting method. Five kinds of dye were used as the additive; p-aminoazobenzene (Ab), p-nitrobenzeneazoresorcinol (Nb), methyl yellow (My), methylene blue (Mb) and methyl red (Mr). In the case of the films with Ab and Mb at 0.05% wt, the magnitude and number of PD pulses were reduced to ~50% less than those in the case of the films without the additive. The decrease in PD was effective under an electric field strength slightly higher than the PD inception. In the case of the other dyes, decrease in PD was not observed. The surface voltage distribution due to the charge by PD depended on the kind of dye additive. The decrease in PD for the Ab and Mb films was explained from the dependence of the surface voltage distribution on the dye. It was concluded that ionization of the dye molecules in the film changes the distribution of surface voltage 相似文献
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8.
The possibility of severe recriticality could be excluded if the molten core materials are discharged from reactor core in the early stage of core disruptive accident (CDA). Based on this idea, several design measures for future commercial liquid metal-cooled fast breeder reactors (LMFBRs) have been proposed to enhance the molten fuel discharge from core in order to prevent formation of the core-wide molten pool with high mobility. One promising concept in these design candidates is modified-FAIDUS (Fuel subassembly with Inner DUct Structure). The event progression in unprotected loss of flow (ULOF) accident in a sodium-cooled large scale FBR with modified-FAIDUS was analyzed to assess the effectual performance of modified-FAIDUS in preventing severe recriticality using the SAS4A and SIMMER-III codes. Two parametric cases were performed covering the uncertainty of duct wall failure mechanism, one with stable fuel crust and another with unstable crust condition. The calculation showed that the final amount of discharged fuel from core in both cases was more than 20% of initial core inventory. The degraded core after fuel discharge is composed of the mixture of solidified fuel, swollen fuel chunks and molten steel, of which low mobility prevents massive fuel motion. The reactor power lowered to decay heat level and the reactivity lowered around −20 $, thus, the possibility of severe recriticality was eliminated. 相似文献
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