全文获取类型
收费全文 | 104篇 |
免费 | 6篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 47篇 |
机械仪表 | 1篇 |
建筑科学 | 8篇 |
矿业工程 | 1篇 |
能源动力 | 6篇 |
轻工业 | 13篇 |
水利工程 | 3篇 |
石油天然气 | 1篇 |
无线电 | 1篇 |
一般工业技术 | 9篇 |
冶金工业 | 2篇 |
原子能技术 | 1篇 |
自动化技术 | 16篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 10篇 |
2021年 | 19篇 |
2020年 | 12篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 6篇 |
2012年 | 6篇 |
2011年 | 7篇 |
2010年 | 4篇 |
2009年 | 3篇 |
2008年 | 3篇 |
2007年 | 2篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2003年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
排序方式: 共有110条查询结果,搜索用时 15 毫秒
1.
Viktoria M. S. Kjær Loukas Ieremias Dr. Viktorija Daugvilaite Dr. Michael Lückmann Prof. Thomas M. Frimurer Prof. Trond Ulven Prof. Mette M. Rosenkilde Dr. Jon Våbenø 《ChemMedChem》2021,16(17):2623-2627
The G protein-coupled receptor GPR183/EBI2, which is activated by oxysterols, is a therapeutic target for inflammatory and metabolic diseases where both antagonists and agonists are of potential interest. Using the piperazine diamide core of the known GPR183 antagonist (E)-3-(4-bromophenyl)-1-(4-(4-methoxybenzoyl)piperazin-1-yl)prop-2-en-1-one (NIBR189) as starting point, we identified and sourced 79 structurally related compounds that were commercially available. In vitro screening of this compound collection using a Ca2+ mobilization assay resulted in the identification of 10 compounds with agonist properties. To enable establishment of initial structure-activity relationship trends, these were supplemented with five in-house compounds, two of which were also shown to be GPR183 agonists. Taken together, our findings suggest that the agonist activity of this compound series is dictated by the substitution pattern of one of the two distal phenyl rings, which functions as a molecular efficacy-switch. 相似文献
2.
Daun Marian Brings Jennifer Krajinski Lisa Stenkova Viktoria Bandyszak Torsten 《Requirements Engineering》2021,26(3):325-370
Requirements Engineering - Collaborative cyber-physical systems are capable of forming networks at runtime to achieve goals that are unachievable for individual systems. They do so by connecting to... 相似文献
3.
Jeremy Rott Eva Teresa Toepfer Maria Bartosova Ana Kolevica Alexander Heuser Michael Rabe Geert Behets Patrick C. DHaese Viktoria Eichwald Manfred Jugold Ivan Damgov Sotirios G. Zarogiannis Rukshana Shroff Anton Eisenhauer Claus Peter Schmitt 《International journal of molecular sciences》2022,23(14)
Serum calcium isotopes (δ44/42Ca) have been suggested as a non-invasive and sensitive Ca balance marker. Quantitative δ44/42Ca changes associated with Ca flux across body compartment barriers relative to the dietary Ca and the correlation of δ44/42CaSerum with bone histology are unknown. We analyzed Ca and δ44/42Ca by mass-spectrometry in rats after two weeks of standard-Ca-diet (0.5%) and after four subsequent weeks of standard- and of low-Ca-diet (0.25%). In animals on a low-Ca-diet net Ca gain was 61 ± 3% and femur Ca content 68 ± 41% of standard-Ca-diet, bone mineralized area per section area was 68 ± 15% compared to standard-Ca-diet. δ44/42Ca was similar in the diets, and decreased in feces and urine and increased in serum in animals on low-Ca-diet. δ44/42CaBone was higher in animals on low-Ca-diet, lower in the diaphysis than the metaphysis and epiphysis, and unaffected by gender. Independent of diet, δ44/42CaBone was similar in the femora and ribs. At the time of sacrifice, δ44/42CaSerum inversely correlated with intestinal Ca uptake and histological bone mineralization markers, but not with Ca content and bone mineral density by µCT. In conclusion, δ44/42CaBone was bone site specific, but mechanical stress and gender independent. Low-Ca-diet induced marked changes in feces, serum and urine δ44/42Ca in growing rats. δ44/42CaSerum inversely correlated with markers of bone mineralization. 相似文献
4.
Automatic image annotation using visual content and folksonomies 总被引:2,自引:4,他引:2
Stefanie Lindstaedt Roland Mörzinger Robert Sorschag Viktoria Pammer Georg Thallinger 《Multimedia Tools and Applications》2009,42(1):97-113
Automatic image annotation is an important and challenging task, and becomes increasingly necessary when managing large image collections. This paper describes techniques for automatic image annotation that take advantage of collaboratively annotated image databases, so called visual folksonomies. Our approach applies two techniques based on image analysis: First, classification annotates images with a controlled vocabulary and second tag propagation along visually similar images. The latter propagates user generated, folksonomic annotations and is therefore capable of dealing with an unlimited vocabulary. Experiments with a pool of Flickr images demonstrate the high accuracy and efficiency of the proposed methods in the task of automatic image annotation. Both techniques were applied in the prototypical tag recommender “tagr”. 相似文献
5.
Aline Bernar Jennifer Viktoria Gebetsberger Monika Bauer Werner Streif Michael Schirmer 《International journal of molecular sciences》2023,24(1)
The alizarin red S assay is considered the gold standard for quantification of osteoblast mineralization and is thus widely used among scientists. However, there are several restrictions to this method, e.g., moderate sensitivity makes it difficult to uncover slight but significant effects of potentially clinically relevant substances. Therefore, an adaptation of the staining method is appropriate and might be obtained by increasing the mineralization ability of osteoblasts. In this study, cell culture experiments with human (SaOs-2) and murine (MC3T3-E1) osteoblasts were performed under the addition of increasing concentrations of calcium chloride (1, 2.5, 5, and 10 mM) or calcitonin (1, 2.5, 5, and 10 nM). After three or four weeks, the mineralization matrix was stained with alizarin red S and the concentration was quantified photometrically. Only calcium chloride was able to significantly increase mineralization, and therefore enhanced the sensitivity of the alizarin red S staining in a dose-dependent manner in both osteoblastic cell lines as well as independent of the cell culture well surface area. This cost- and time-efficient optimization enables a more sensitive analysis of potentially clinically relevant substances in future bone research. 相似文献
6.
Olena V. Moshynets Taras P. Baranovskyi Olga S. Iungin Nadiia P. Kysil Larysa O. Metelytsia Ianina Pokholenko Viktoria V. Potochilova Geert Potters Kateryna L. Rudnieva Svitlana Y. Rymar Ivan V. Semenyuta Andrew J. Spiers Oksana P. Tarasyuk Sergiy P. Rogalsky 《International journal of molecular sciences》2022,23(2)
The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here, we investigated how different biocides affect the release of eDNA from mature biofilms of two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles. The effect of four groups of biocides, alcohols, hydrogen peroxide, quaternary ammonium compounds, and the polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl), was evaluated using PA and SA biofilms. Most biocides, except for PHMG-Cl and 70% ethanol, caused substantial eDNA release, and PHMG-Cl was found to block biofilm development when used at concentrations of 0.5% and 0.1%. This might be associated with the formation of DNA–PHMG-Cl complexes as PHMG-Cl is predicted to bind to AT base pairs by molecular docking assays. PHMG-Cl was found to bind high-molecular DNA and plasmid DNA and continued to inactivate DNA on surfaces even after 4 weeks. PHMG-Cl also effectively inactivated biofilm-associated antibiotic resistance gene eDNA released by a pan-drug-resistant Klebsiella strain, which demonstrates the potential of a polymeric biocide as a new surface-active agent to combat the spread of antibiotic resistance in hospital settings. 相似文献
7.
Marie Ebeyer-Masotta Tanja Eichhorn Ren Weiss Vladislav Semak Lucia Laukov Michael B. Fischer Viktoria Weber 《International journal of molecular sciences》2022,23(3)
Inflammation and thrombosis are closely intertwined in numerous disorders, including ischemic events and sepsis, as well as coronavirus disease 2019 (COVID-19). Thrombotic complications are markers of disease severity in both sepsis and COVID-19 and are associated with multiorgan failure and increased mortality. Immunothrombosis is driven by the complement/tissue factor/neutrophil axis, as well as by activated platelets, which can trigger the release of neutrophil extracellular traps (NETs) and release further effectors of immunothrombosis, including platelet factor 4 (PF4/CXCL4) and high-mobility box 1 protein (HMGB1). Many of the central effectors of deregulated immunothrombosis, including activated platelets and platelet-derived extracellular vesicles (pEVs) expressing PF4, soluble PF4, HMGB1, histones, as well as histone-decorated NETs, are positively charged and thus bind to heparin. Here, we provide evidence that adsorbents functionalized with endpoint-attached heparin efficiently deplete activated platelets, pEVs, PF4, HMGB1 and histones/nucleosomes. We propose that this elimination of central effectors of immunothrombosis, rather than direct binding of pathogens, could be of clinical relevance for mitigating thrombotic complications in sepsis or COVID-19 using heparin-functionalized adsorbents. 相似文献
8.
Seksan Udomsri Chris BalesAndrew R. Martin Viktoria Martin 《Energy and Buildings》2011,43(12):3311-3321
This article presents the monitoring results of a thermally driven chiller (TDC) driven by district heat from a network supplied by a centralised combined heat and power (CHP) fired with municipal waste. The main objective of this article is to analyse the monitoring results obtained from the demonstration and calibrate a system model that is later used for parametric studies in order to find improved system design and control. The calibration of the system model was made in three stages and all the energy performance figures were within 4% of the measured values. Results show that the TDC system is capable of providing maximum thermal and electrical COP's of 0.50 and 4.6 respectively during the hottest period. For the complete monitoring period during the summer of 2008, the figures were 0.41 and 2.1. The lower figures were due to continuous pump operation inside the TDC even during periods of no cold production and a period when no cold was produced. However the internal pumps inside the TDC have been removed in the new version TDC to increase the electrical COP. System simulation and parametric studies will be employed to further determine how the electrical COP can be improved. 相似文献
9.
Dipl.-Ing. Gunter Gschwandtner Robert Galler Viktoria Wörgetter Franz Riepler Sylke Hilberg 《BHM Berg- und Hüttenm?nnische Monatshefte》2013,158(2):53-59
The assessment of the stability of underground structures is an interdisciplinary task which consists of geology, hydrogeology, surveying and geotechnical engineering. This paper gives an example of how these disciplines, their methods and the acquisition of data work together to create a complex three-dimensional computer-model of underground structures. It demonstrates, that especially the complex layout of old and “grown” underground structures cannot be represented accurately in two-dimensional analyses or models and provide no realistic results for stability investigations. Related to the stability of underground structures, a non-negligible factor is the influence of water, whether surface, rain or groundwater. The occurring leaching process of gypsum leads to a weakening of the surrounding rock which causes a strength reduction. This softening of the material must be considered in long-term simulations. The presented comprehensive approach tries to provide a possibility to predict the time-dependent occurrence of vulnerabilities in the rock mass and thus gives the possibility to install support measures in an early stage. 相似文献
10.
Water kefir is a home made fermented beverage based on a sucrose solution with fruit extracts. The inoculum of such fermentations consists of macroscopic granula containing lactic and acetic acid bacteria, and yeasts, which are embedded in an exopolysaccharide (EPS) matrix. In this work, a strain of Lactobacillus hilgardii producing large amounts of the granule-forming dextran could be isolated. The glycosyltransferans (Gtf) commonly called glucansucrase responsible for the production of this dextran was purified from L. hilgardii. Characteristic enzyme kinetic data were obtained. Optimum activity was observed between pH 4.3 and 4.6 and temperatures between 40 °C and 45 °C. A Michaelis–Menten kinetic could be fit to the experimental data and a KM of 0.0385 M was calculated. The corresponding gtf gene was identified and characterized. It encodes a 1448 amino acid protein with higher homologies to Gtfs of Lactobacillus parabuchneri, Lactobacillus sakei and Lactobacillus fermentum followed by lower homologies to Lactobacillus reuteri Gtfs. By knockout experiments the role of this gene in granule dextran production was demonstrated. 相似文献