Preparation of fluorescence starch-nanoparticle and its application as plant transgenic vehicle

Starch-nanoparticles were synthesized in water-in-oil microemusion at room temperature, and the starch-nanoparticles were coated with poly-L-lysine. The surface of the starch-nanoparticles was combined with fluorescence material Ru（bpy）3^2＋-6H2O, and then the particles were characterized via transmission electron microscope. The fluorescence nanoparticles were conjugated with plasmid DNA to form complexes, and then treated with ultrasound and DNase I. pEGAD plasmid DNA-nanoparticle complexes were co-cultured with plant suspension cells ofDioscrea Zigiberensis G H Wright, and treated with ultrasound. The results show that the diameter of the fluorescence starch-nanoparticles is 50-100 nm. DNA-nanoparticle complexes can protect DNA from ultrasound damage as well as from DNase I cleavage. Mediated by ultrasound, pEGAD plasmid DNA-nanoparticle complexes can pierce into the cell wall, cell membrane and nucleus membrane of plant suspension cells. The green fluorescence protein（GFP） gene at a high frequency exceeds 5%. This nano-biomaterial can efficiently solve the problem that exterior genes cannot traverse the plant cell wall easily.     

Synthesis and biochemical properties of fluorescent/magnetic bifunctional starch particles

Magnetic starch particles(MSPs) were synthesized in water-in-oil microemulsion at room temperature.MSPs were characterized by transmission electron microscopy(TEM),Fourier transform infrared spectrometry(FTIR),zeta potential system,thermogravimetric analysis(TGA) and vibrating sample magnetometry(VSM).The average diameter of the MSPs was 220 nm,dispersed with well-proportioned size and magnetic resonance,the saturation magnetization was 3.64 A·m2/kg.MSP was coated with poly-L-lysine(PLL),and then the surfac...