蚯蚓蛋白质组双向电泳技术体系的建立及条件优化

对赤子爱胜蚓(Eisenia fetida)表皮组织的两种蛋白质提取方法进行了比较,并对双向电泳IPG胶条pH范围,上样量条件进行了优化,结果表明:裂解液提取法提取的蛋白质量浓度较低,蛋白种类缺失,采用TCA-丙酮沉淀法提取法,可显著提高提取液蛋白质提取效果,样品中蛋白质质量浓度可达12.49μg/μL.蚯蚓表皮组织蛋白质主要分布在pH范围4～7之间,适于选用24cmpH 4～7线性梯度IPG胶条,采用考马斯亮蓝染色法时最适上样量为600μg,所得电泳图谱可检测蛋白点达(629±20)个,且重复性实验匹配率较高.用TCA-丙酮沉淀法提取蚯蚓表皮组织蛋白质组,按优化的参数进行双向电泳分离,所得图谱分辨率高、重复性好,可用于蚯蚓蛋白质组学研究.     

基于高盐条件下的Bacillus circulans WZ-12 CCTCC M 207006好氧降解氯化碳氢化合物(英文)

A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions.CH2Cl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L-1 to 10 g·L-1,and the maximum degradation efficiency(85%) was achieved at NaCl concentration of 10 g·L-1.Similarly,Bacillus circulans WZ-12 was able to degrade CH2 BrCl,C2H4Cl2,and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h.Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine(GB),but GB levels were 3-to 5-fold higher in cells grown in media with high salt.Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation(R 2-0.85-0.94) was observed between the rate of biodegradation(V) and salt concentration from 5 g·L-1 to 60 g·L-1.The growing cells(in minimal salt medium) degraded approximately 50% of the CH2Cl2 within 24 h,whereas the resting cells(in physiological saline) degraded only 25% of the CH2Cl2 within 24 h and were inactive after 36 h cultivation.Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency.Bacillus circulans WZ-12 grows well in an aqueous/oil system,hence,it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.     

Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CH₂Cl₂ was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L^-1 to 10 g·L^-1, and the maximum degradation efficiency (85%) was achieved at NaCl concentration of 10 g·L^-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH₂BrCl, C₂H₄Cl₂, and C₂H₂Cl₂ in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine (GB), but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation (R² 0.85-0.94) was observed between the rate of biodegradation (V) and salt concentration from 5 g·L^-1 to 60 g·L^-1. The growing cells (in minimal salt medium) degraded approximately 50% of the CH₂Cl₂ within 24 h, whereas the resting cells (in physiological saline) degraded only 25% of the CH₂Cl₂ within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.     

公路钢桥设计荷载超声波焊缝检测标准研究

目前公路钢桥焊缝超声波检测验收标准,完全照搬铁路钢桥检测标准,但是公路与铁路钢桥荷载等级不同,铁路桥梁的标准相对于公路桥梁比较高,对焊缝要求也较高。本文通过对比铁路桥梁和公路桥梁的荷载等级进行了公路钢桥超声波焊缝检测标准研究。     

重载交通条件下装配式RC板桥抗裂性分析

为研究重载交通条件下RC板桥的抗裂性能,以煤运干线宣大高速公路某大桥所采集的动态称重（WIM）数据为基础,提取出883个特重车荷载工况,借助自主研发的三维动力可视化分析软件BDANS计算特重车荷载工况作用下RC板桥的正弯矩效应,并按照现行桥梁规范的裂缝宽度计算方法计算每个特重车工况作用下RC板桥跨中截面的裂缝宽度。结果表明:RC板桥跨中截面的裂缝宽度均介于0.1~0.2 mm之间,未超越现行桥梁规范所规定的最大裂缝宽度,但该类型裂缝的存在将为混凝土碳化、钢筋的电化学腐蚀提供有利的发生环境;为保证结构的正常使用并延长其服役期,应对已有裂缝进行修复处理并对公路桥梁车辆荷载进行限载研究。     

基于可靠度理论的中小跨径桥梁卡车载重限值研究

卡车载重限值标准直接影响实际汽车荷载水平及其分布特性以及桥梁等基础设施的服役性能。为分析中小跨径桥梁的卡车载重限值问题,该文首先基于可靠度理论建立卡车载重限值分析模型;其次,基于长期记录的WIM（Weigh in Motion）数据分析我国典型公路路段卡车荷载特性并确定需要进行载重限值研究的重载卡车车型,并根据我国当前中小跨径桥梁建设现状选择对卡车载重限值起控制作用的桥梁结构类型;最后,建立结构关键截面抗弯功能函数,选择多层次目标可靠指标,对各类型卡车的载重限值进行反向迭代分析,基于分析结果分别讨论目标可靠指标、区域荷载特性对卡车载重限值的影响,以及现行公路治超标准对中小跨径桥梁车辆限载的适用性。分析结果显示:各类型卡车载重限值随着目标可靠指标的增大有明显线性下降趋势;区域卡车荷载特性的差异对卡车载重限值有着不可忽略的影响;现行国家治超标准对中小跨径桥梁车辆限载具有较好的适用性,但建议各地区根据自身实际情况在国家公路治超标准的基础上进行微调。