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针对实践中,农村食品安全治理存在着基层政府隐性缺失、村级组织治理能力有限和自主性缺失、食品安全意识不足、软治理机制虚化等主要症结,提出推进农村食品安全软治理模式,指出需要厘定政府属地责任、强化政府农村食品安全治理职能;加强村级组织食品安全治理能力建设和食品安全宣传教育;构建农村食品安全软治理机制,促进社会合作,提升治理实效,弥补现行农村食品安全硬治理存在的不足。 相似文献
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A Lactobacillus brevis CGMCC 1306 isolated from fresh milk without pasteurization was found to have higher glutamate decarboxylase (GAD) activity. An effective isolation and purification procedure of GAD from a cell-free extract of Lactobacillus brevis was developed, and the procedure included four steps: 30%-90% saturation (NH4)2SO4 fractional precipitation, Q sepharose FF anion-exchange chromatography, sephacryl S-200 gel filtration, and resource Q anion-exchange chromatography. Using this protocol, the purified GAD was demonstrated to possess electrophoretic homogeneity via SDS-PAGE. The purification fold and activity recovery of GAD were 43.78 and 16.95%, respectively. The molecular weight of the purified GAD was estimated to be approximately 62 kDa via SDS-PAGE. The optimum pH and temperature of the purified GAD were 4.4 and 37℃, respectively. The purified GAD had a half-life of 50rain at 45℃ and the Km value of the enzyme from Lineweaver-Burk plot was found to be 8.22.5'-pyridoxal phosphate (PLP) had little effect on the regulation of its activity. 相似文献
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淡水鱼连续式去头尾装置的研制 总被引:2,自引:0,他引:2
为了实现淡水鱼的高效去头尾加工功能,研制一种由间歇输送系统、刀具夹具驱动系统、控制系统等组成的淡水鱼连续式去头尾装置。该装置配套功率2.3kW,其中间歇输送系统电机1.5kW,刀具夹具驱动系统空气压缩机0.75kW,控制系统等0.05kW。以(0.400±0.100)kg鳊鱼,(1.200±0.300)kg草鱼为研究对象,进行装置加工效果评价试验。试验结果表明,去头尾装置加工鳊鱼得率为82.10%,草鱼得率为76.30%,加工鳊鱼感官评分为0.81,加工草鱼感官评分为0.79,加工鳊鱼成功率为90.00%,加工草鱼成功率为80.00%,加工效率可达1 350条/h。 相似文献
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