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Dr. Vlastimil Jirasko Dr. Nils-Alexander Lakomek Dr. Susanne Penzel Dr. Marie-Laure Fogeron Prof. Dr. Ralf Bartenschlager Prof. Dr. Beat H. Meier Dr. Anja Böckmann 《Chembiochem : a European journal of chemical biology》2020,21(10):1453-1460
Proton-detected 100 kHz magic-angle-spinning (MAS) solid-state NMR is an emerging analysis method for proteins with only hundreds of microgram quantities, and thus allows structural investigation of eukaryotic membrane proteins. This is the case for the cell-free synthesized hepatitis C virus (HCV) nonstructural membrane protein 4B (NS4B). We demonstrate NS4B sample optimization using fast reconstitution schemes that enable lipid-environment screening directly by NMR. 2D spectra and relaxation properties guide the choice of the best sample preparation to record 2D 1H-detected 1H,15N and 3D 1H,13C,15N correlation experiments with linewidths and sensitivity suitable to initiate sequential assignments. Amino-acid-selectively labeled NS4B can be readily obtained using cell-free synthesis, opening the door to combinatorial labeling approaches which should enable structural studies. 相似文献
3.
The present study investigates the impact of different key solutions of mobile phones on users' effectiveness and efficiency using the devices. In the first experiment, 36 children (9 - 14 years) and in the second experiment 45 young adults (19 - 33 years) completed four common phone tasks twice consecutively on three simulated phones that had identical menus, but different key solutions. An approach was undertaken to quantify the complexity of keys in three models, incorporating different factors contributing to the keys' complexity (number of key options, number of modes and number of modes with a semantically dissimilar meaning), in order to predict users' performance decrements. As a further main factor, the degree of the users' locus of control (LOC) was measured and interactions with performance outcomes were studied. As dependent measures, the number of inefficient keystrokes, the number of tasks solved and the processing time were determined. Results showed a significant effect of control key solutions on users' efficiency and effectiveness for both children and young adults. Moreover, children's LOC values significantly interacted with performance: children with low LOC values showed the lowest performance and no learnability, especially when using keys with a high complexity. From the three factors contributing to the complexity of keys, keys exerting different functions with semantically inconsistent meanings had the worst effect on performance. It is concluded that in mobile user interface design keys with semantically inconsistent meanings should be generally avoided. 相似文献
4.
This study, which is based on responses from over 300 individuals in England and Wales, explores how a willingness to use a water-recycling system of specific configuration varies against a number of factors which are linked to system design, system scale, system context, and water-conservation behaviour. Findings show that there is broad willingness to accept in-house water recycling provided that public health is not compromised. 相似文献
5.
Anguilla is a UK overseas (non-independent) territory in the Caribbean. It is a small middle-income island with a population of 13 000, and recent statistics indicate that it is more dependent on tourism than any other country or territory in the world. Rapid population growth and development have placed more emphasis on the need for human health and environmental protection. For the first time, a systematic water-quality monitoring programme has been developed, based on the realities of the local situation, e.g. low resource base, limited capabilities, dependence on household rainwater catchment, storage cisterns, and a poor water-supply network. Development of the programme incorporated the key themes of pragmatic, realistic, incremental, responsive, and consultative approaches. 相似文献
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Ohne Zusammenfassung 相似文献
7.
Kyle A. Crawford
Osama K. Eyada PhD PE
《Computers & Industrial Engineering》1989,17(1-4):298-302The worldwide proliferation of fraudulent materials has brought about the need for a new approach to the control of purchased material quality. Nuclear power, defense, aerospace and many other industries are affected by the supply of poor quality clones that presume to be original replacement parts. Safety considerations abound as these highly defective materials are used in systems that are critical to the preservation of human lives. Commonly utilized quality assurance programs have not effectively stopped the influx of fraudulent materials. These programs fail to concentrate inspection efforts on materials and inspection attributes that would effectively prevent the acceptance of fraudulent materials. herein a solution is presented to this problem in the form of an expert system application. Information commonly available in industry is formulated into a knowledge based system wherein advisories are given to the user regarding key purchased material receipt inspection strategies. 相似文献
8.
Water turbidity, surface temperature and circulation of Barton and Wroxham Broads, two of the Norfolk Broads, were studied using one reflective (TM1) and the thermal (TM6) TM band. The results indicated that the through-flowing path of the River Ant in Barton Broad is the major influence on the distribution of these parameters. In Wroxham Broad, both turbidity and surface temperature displayed a uniform distribution. This could be explained by the absence of a predominant direction of flow. 相似文献
9.
During the day, there are considerable variations in the climatic factors prevailing in these regions. This is especially so during the 24-hour daily cycle of the long, hot and dry summer (mid-May to midOctober). Such climatic conditions have had a considerable impact on the daily living pattern of family life in the same season, particularly in the urban areas. During the same 24-hour cycle, they have compelled the inhabitants to shift their living activities both vertically in section and horizontally in plan; they do this in pursuit of more acceptable, if not, desirable internal thermal environmental ambience. 相似文献
10.
Document caching and connection caching are extensively studied problems. In document caching, one has to maintain caches
containing documents accessible in a network. In connection caching, one has to maintain a set of open network connections
that handle data transfer. Previous work investigated these two problems separately while in practice the problems occur together:
In order to load a document, one has to establish a connection between network nodes if the required connection is not already
open. In this paper we present the first study that integrates document and connection caching. We first consider a very basic
model in which all documents have the same size and the cost of loading a document or establishing a connection is equal to
1. We present deterministic and randomized online algorithms that achieve nearly optimal competitive ratios unless the size
of the connection cache is extremely small. We then consider general settings where documents have varying sizes. We investigate
a FAULT model in which the loading cost of a document is 1 as well as a BIT model in which the loading cost is equal to the
size of the document. 相似文献