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使用摇瓶发酵制备解淀粉芽孢杆菌,喷雾干燥将其制备成菌粉。采用单因子实验首先对影响摇瓶发酵制备解淀粉芽孢杆菌的条件因素进行优化,然后采用单因子实验和正交实验,对影响喷雾干燥产物指标的因素进行优化。优化后的摇瓶发酵条件为:5g/L可溶性淀粉、10g/L氮源(酵母粉与蛋白胨体积比为2:1)、接种量7%、摇瓶装液量40%,摇床200r/min、30℃条件下摇瓶发酵48h,菌液活菌数为8.3×108CFU/mL,优于优化前的3.8×108CFU/mL。优化后的操作条件为:进风温度180℃、热风流量315m3/h、进样速度500mL/h、麦芽糊精质量分数5%。各因素对其喷雾干燥工艺的影响程度为:麦芽糊精浓度 > 进料速度 > 进风温度 > 空气流量。在此条件下,解淀粉芽孢杆菌菌粉活菌数可达1.28×1010CFU/g。 相似文献
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低浓度污水生物处理的进展 总被引:1,自引:1,他引:0
由于厌氧处理技术的进展,近来已开始研究低浓度污水的厌氧处理,但低浓度污水(BOD_5<500mg/L)的厌氧处理与高浓度废水的处理情况不同,一是污水浓度低,二是常温甚至低温条件下运行,一般25~10C。 相似文献
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Wastewaters from the chemical industry are usually of high-strength and may contain minor inhibitory and recalcitrant organics that are at times not readily identifiable. This paper describes the experience of a biological waste water treatment plant (WWTP) processing a COD concentration of 43000 mg·L^-1 wastewater from an oxochemical manufacturing plant. Stage improvements of the plant process by dilution of the inhibitory influent using other chemical wastewater streams resulting in a synergistic process effect, and removal of inhibitory organics by phase separation via acidification, effectively achieved process optimization producing a high quality effluent. In particular, the COD removal efficiency of granular sludge based anaerobic reactors increased from 56% to 90%. The final effluent COD decreased from 250mg·L^-1 to 50mg·L^-1, consistently meeting the COD concentration of 100 mg·L^-1 regulatory discharge limit. The success of the process enhancements supports the hypothesis that long-chain quaternary carboxylic acids act as substrate inhibitors in the biological process. 相似文献
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本文讨论了以工业葡萄糖液;啤酒废水及酒糟废液为基质、采用一相两相厌氧工艺连续及间歇运行的试验结果。表明相分离能通过调节厌氧生态系统中各种生物群体的相对数量,强化污泥活性及运行稳定性,两相厌氧工艺特别适宜于处理高浓度(有机负荷决定反应器运行)溶解性有机废水。 相似文献
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