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利用菌株对(维生素K3+叠氮化钠+对羟基苯甲酸)的复合抗性作为筛选标记,对产辅酶Q10的类球红细菌(Rhodobacter sphaeroides)使用亚硝基胍进行化学诱变,筛选高产菌株并采用响应面法优化其发酵培养基。结果表明,经诱变选育得到一株遗传稳定的辅酶Q10高产菌株R.sp3-7,其最佳发酵培养基组成为:葡萄糖31.7 g/L,玉米浆干粉5.6 g/L,(NH4)2SO4 5.3 g/L、谷氨酸钠 3.0 g/L、NaCl 3.0 g/L、MgSO4·7H2O 12.5 g/L、KH2PO4 3.0 g/L、CaCO3 2.0 g/L、辅液1 mL/L。在此培养条件下,诱变菌株R.sp3-7的辅酶Q10产量达(93.63±0.59) mg/L,与出发菌株相比提高了116.8%。
关键词:中图分类号:TS201.3 文章编号:0254-5071(2016)06-0090-06 doi: 相似文献
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不同来源SOD的制备及作用机理 总被引:2,自引:0,他引:2
系统综述了自然界中存在的 SOD类型及作用机理 ;介绍了动物、植物、微生物中SOD的制备工艺 ,并对工艺存在的问题进行了评述。 相似文献
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The effects of nitrogen sources on streptolydigin production and distribution of secondary metabolites were investigated for flask cultured S.lydicus AS 4.2501.When peptone,asparamide,and glutamic acid were ex- amined as the nitrogen source,respectively,liquid chromatography-mass spectrometry(LC-MS)and photodiode array(PDA)analyses revealed the formation of two analogues of streptolydigin in the fermentation broth.When soybean meal was used as the source of nitrogen,three analogues of streptolydigin were detected.The use of am- monium sulfate as a source of nitrogen resulted in a lower pH value of the fermentation system,thus inhibiting streptolydigin biosynthesis and changing the metabolic profiling.Among the nitrogen sources that were made use of,glutamic acid was most favorable to the formation of streptolydigin.Simultaneously,this study also showed that the changing nitrogen sources resulted in altering the production and relative ratios of streptolydigin and its analogues. 相似文献
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羟基乙叉二膦酸的快速分析 总被引:1,自引:0,他引:1
本文介绍了羟基乙叉二膦酸的结构、性质及应用,同时对分析方法进行了研究。 相似文献
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微乳液中酶催化木素-酚共聚物的性能研究 总被引:1,自引:0,他引:1
用过氧化物酶进行了酶促木素与酚共聚反应。结果表明 ,由于在木素分子间引入了聚甲酚片段 ,共聚物的热性能有较大改善。聚合物的重均分子量随反应温度的升高而增加 相似文献