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The adsorption of protein from model wine was investigated under different temperatures, pH values, contact times, and concentrations of ethanol, by certain bentonites. The results showed that ethanol molecules could broaden the protein molecules' channel to the interlayer of bentonite, and the maximum protein adsorption amount occurred under an ethanol concentration of 12% (by volume) and a pH value of 3.56. The increased single point Brunauer-Emmitt-Teller (BET) surface area (SBET) and adsorption pore volume (VAds) suggested a larger amount of active adsorption sites of the bentonite surface and a wider protein channel from the surface to the inner adsorption sites of bentonite, respectively. At the same time, higher methylene blue test (MBT) and swelling index (Sw) indicated that it was easy for the entrance of water and the absorbance of protein. Higher temperature was found favorable to eliminate more proteins and it took about 20 to 40min to arrive at the maximum adsorption. 相似文献
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以N,N'-亚甲基双丙烯酰胺为交联剂,过硫酸钾为引发剂,丙烯酸(AA)和丙烯酰胺(AM)为单体,采用水溶液聚合法合成出钠基膨润土复合聚丙烯酸钠-丙烯酰胺复合吸水树脂.研究了钠基膨润土的添加量、交联剂用量、引发剂用量、中和度及AM的用量对吸水倍率的影响.结果表明,钠基膨润土用量为40%,交联剂用量为0.025%,引发剂用量为0.3%,AM用量为12%及AA的中和度为80%时,吸蒸馏水倍率与吸收0.7%NaCl溶液倍率分别为422.28 g/g和65.80 g/g. 相似文献
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高效液相色谱法(HPLC)快速检测辣椒中的辣椒红素 总被引:2,自引:0,他引:2
建立了辣椒中辣椒红色素的高效液相色谱法定性定量检测方法。结果表明,采用v(正己烷)v∶(丙酮)=782∶2为最佳流动相,检测波长475nm,分析柱Shim-pack CLC-SIL(M)(150×4.6mmID),流速:0.8mL/min,柱温40℃,使辣椒红色素在10min内得到了较好的分离。线性范围为0.022 ̄0.176mg/mL,r=0.9994,平均回收率为99.17%,变异系数为1.63%(n=5),最小检测限为10ng。该方法具有简单快速、精密度高等优点。 相似文献
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The textural properties of acid-activated bentonite (AAB), which were prepared using four different concentrations of sulfuric acid, were analyzed by adsorption-desorption isotherm of nitrogen using an automated specific surface area and porosity analyzer. The total pore volume, specific surface area and average pore diameter of these four kinds of AAB show a regular changing trend, increasing first and then decreasing, the optimum of which can be achieved at a sulfuric acid concentration of 25% (sample A25). The kinetic analysis of the adsorption ofβ-carotene and chlorophyll in model oil solutions, namely, xylene and edible oil solution, has been investigated by using AAB. Experimental results indicated that the adsorption data fit the pseudo-second-order model well. The whole adsorption process of the two pigments on AAB was divided basically into two parts the initial adsorption of pigments was rapid in the first 10 min, followed by a slower adsorption process till equilibrium was attained at 60 min. In addition, the amount and rate of adsorption on A25 increase synchronously with the initial pigment concen-tration and temperature. The results showed that the adsorption kinetics behavior of AAB with respect to the pig- ments is not influence by the xylene and edible oil solution. 相似文献