氯化亚铜/乙二胺/苄氯引发苯乙烯聚合

研究了以氯化亚铜/乙二胺/苄氯体系引发苯乙烯聚合反应,可以得到数均分子量高达2.3×105,分子量分布窄(MWD<1.6)的聚合产物。在聚合反应时间6h左右聚合转化率可以高达90%,在聚合温度90℃～120℃范围内,聚合物分子量分布均小于1.63;测定聚合反应动力学方程为Rp=kp.[M].[Y]1/2,聚合反应表观活化能为50.13kJ/mol。     

荔枝核黄酮类化合物的提取工艺研究

采用回流、热浸提、微波提取等工艺提取荔枝核黄酮类化合物,采用正交实验考察了提取溶剂浓度、提取时间、微波功率、提取温度、料液比等因素对提取率的影响,优选出不同工艺的提取条件。回流提取工艺的优化条件是:50%乙醇溶液,提取温度80℃,提取时间1h,料液比1∶10,提取3次,总黄酮得率为6.76%。热浸提工艺的优化条件是:50%乙醇溶液,提取温度70℃,提取时间1.5h,料液比1∶12,提取2次,总黄酮得率达到6.37%。微波提取工艺的优化条件是:60%乙醇溶液,微波功率700W,提取时间2.5h,料液比1∶25,总黄酮得率达到6.86%。     

Microwave assisted-semi bionic extraction of lignan compounds from Fructus Forsythiae by orthogonal design

Microwave assisted-semi bionic extraction （MASBE） process for lignans from Fructus Forsythiae was studied. The influences of solvent pH value, microwave power, dosage of solvent and irradiation time were investigated. Optimum extracting parameters were determined by orthogonal experiments as follows： pH value of solvent at first extraction is 5.5-6.0, that at the second extraction is 7-8; microwave power is 700 W, mass ratio ofFructus Forsythiae to water is 1：12; irradiation time is 10 min, and extracting times is two. Under these optimal conditions, the yield of lignans reaches 0.364%. Compared with the conventional extraction methods, the MASBE process has the advantages of high extraction rate, high extraction selectivity.     

酶-膜法提取纯化荔枝核中氨基酸

采用酶-膜法提取纯化荔枝核中氨基酸。使用X JT9503中性蛋白酶酶解辅助提取荔枝核中氨基酸,实验确定酶解的优化条件为:酶用量600 U/g、酶解温度50℃、pH=6.5、酶解时间90 m in,氨基酸得率为1.16%。采用水提法和酸解提取法氨基酸得率分别仅为0.41%和0.92%。实验表明,酶解提取法要优于水提法和酸解提取法。使用截留相对分子质量50 000的陶瓷超滤膜纯化酶解提取液,实验表明,膜通量随操作压力和料液温度升高而增加。在操作压力0.22 MPa,料液温度30℃的条件下,膜的平均通量为75.63 L/(m2.h),氨基酸的截留率仅为10.3%,蛋白质的截留率为98.1%,多糖的截留率为85.2%,氨基酸能够与蛋白质、多糖等大分子实现有效分离。