排序方式: 共有3条查询结果,搜索用时 0 毫秒
1
1.
Improvement of Vitamin K2 Production by Escherichia sp.with Nitrogen Ion Beam Implantation Induction
Low-energy ion implantation as a novel mutagen has been increasingly applied in the microbial mutagenesis for its higher mutation frequency and wider mutation spectra.In this work,N~+ ion beam implantation was used to enhance Escherichia sp.in vitamin K_2 yield.Optimization of process parameters under submerged fermentation was carried out to improve the vitamin K_2yield of mutant FM5-632.The results indicate that an excellent mutant FM5-632 with a yield of 123.2±1.6 μg/L,that is four times that of the original strain,was achieved by eight successive implantations under the conditions of 15 keV and 60×2.6×l0~(13)ions/cm~2.A further optimization increased the yield of the mutant by 39.7%,i.e.172.1±1.2 μg/L which occurred in the mutant cultivated in the optimal fermentation culture medium composed of(per liter):15.31 g glycerol,10 g peptone,2.89 g yeast extract,5 g K2HPO_4,1 g NaCl,0.5 g MgSO_4·7H_2O and 0.04 g cedar wood oil,incubated at 33℃,pH 7.0 and 180 rpm for 120 h. 相似文献
2.
L-谷氨酰肼为底物酶法制备茶氨酸 总被引:1,自引:0,他引:1
采用一种廉价的底物L-谷氨酰肼代替L-谷氨酰胺,成功地合成了茶氨酸,并且对该酶促反应的条件进行了初步优化。结果表明,以L-谷氨酰肼为底物时,γ-谷氨酰转肽酶(GGT)活性约是以L-谷氨酰胺为底物时的78.3%。该酶促反应的最适条件为:pH=10,反应温度40℃,n(L-谷氨酰肼)∶n(乙胺)=1∶10,L-谷氨酰肼初始浓度0.3mol/L。利用以上条件进行了茶氨酸的小量制备,投料L-谷氨酰肼50g,乙胺140g,反应40h,L-谷氨酰肼的摩尔转化率达84.1%,经离子交换树脂分离产物,得到31.8g高纯度茶氨酸。该结果显示良好应用前景。 相似文献
3.
1