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Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) is a rather new ‘soft ionization’ techniques. Because of its high sensitivity and accuracy, it has been widely used in detection and characterization of macromolecules such as peptides, proteins and olignucleotides. It also has been applied successfully in the analysis of posttranslational modification of proteins, such as phosphorylation and glycosylation. Compared with the conventional chemical methods, mass spectrometry is simple, rapid and does not require radiolabeling. In this research, a systematic method to analyse glycoprotein by MALDI-TOF-MS was developed. A practical sample--recombinant human erythropoietin was analysed by the method. The molecular weight, content of carbohydrate and glycosylation sites of the glycoprotein were determined by MALDI-TOF-MS, combined with the endo-glycosidase digestion and peptide mapping. The experimental result shows that MALDI-TOF-MS is a very powerful technique in the characterization of glycosylation proteins. 相似文献
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本文采用低输入信号、高精度的32路单端输入、12位PC6318AD数据采集卡,采用Visal Basic3.2编写数据采集与自理软件,实现了多个物理化学;实验的计算机数据采集、处理与网络实验教学管理。其实验结果与文献值个吻合。 相似文献