Preparation and characterization of proton‐exchange hybrid membranes

Two types of composite were prepared, based on a thermoplastic polymer, polyvinylidene fluoride (PVDF), and an elastomer, ethylene‐propylene‐diene terpolymer (EPDM), respectively. We obtained both series by addition of an inorganic proton‐conducting antimonic acid derivative (HSb) and polystyrene crosslinked with a small percentage of divinylbenzene (PS‐co‐DVB). From these composites, membranes were obtained and subjected to a heterogeneous‐phase sulfonation reaction with chlorosulfonic acid. All experimental materials were characterized from a morphological and electrical point of view, by means of techniques such as differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), non‐isothermal crystallization and complex impedance analysis. Copyright © 2005 Society of Chemical Industry     

Fullerene Reactivity in an Oxygen Plasma

Fullerene reactivity in an oxygen plasma was measured and compared with those determined under the same conditions for a comprehensive set of 50 carbon materials. The possible reasons for the high plasma reactivity of the fullerene sample in an oxygen plasma are discussed.     

Standard goal programming with fuzzy hierarchies: a sequential approach

This paper proposes a pragmatic model for multi-objective decision-making processes involving clusters of objectives which have a decisional meaning for the decision maker (DM). We provide the DMs with a comfortable tool that allows them to express their preferences both by comparing criteria of the same cluster and via the comparison between the different clusters. In standard goal programming the importance of the goals is modeled by the introduction of preferential weights or/and the incorporation of pre-emptive priorities. However, in many cases the DM is not able to establish a precise preference structure. Even in the case of precise weights the solution does not match necessarily the relative weights or, in the case of precise pre-emptive priority, the result could be very restrictive. In order to overcome these drawbacks, in this paper the normalized unwanted deviations are interpreted in terms of achievement degrees of the goals and fuzzy relations are used to model the relative importance of the goals. Thus, we show how several methodologies from the fuzzy goal programming literature can be tailored for solving standard GP problems. We apply this new modeling to problems where there is a “natural” clustering between goals of the same class. We address this situation by solving two phases; in the first one each class is handled separately taking into account the hierarchy of their goals and, in the second phase, we integrate the results of the first phase and the imprecise hierarchy of the different classes. We formulate a new goal programming model called as sequential goal programming with fuzzy hierarchy model. Because many real situations involve decision making in this environment, our proposal can be a useful tool of broad application. A numerical example illustrates the methodology.     

Cell-protection mechanism through autophagy in HGFs/<Emphasis Type="Italic">S. mitis</Emphasis> co-culture treated with Chitlac-nAg

Silver-based products have been proven to be effective in retarding and preventing bacterial growth since ancient times. In the field of restorative dentistry, the use of silver ions/nanoparticles has been explored to counteract bacterial infections, as silver can destroy bacterial cell walls by reacting with membrane proteins. However, it is also cytotoxic towards eukaryotic cells, which are capable of internalizing nanoparticles. In this work, we investigated the biological effects of Chitlac-nAg, a colloidal system based on a modified chitosan (Chitlac), administered for 24–48?h to a co-culture of primary human gingival fibroblasts and Streptococcus mitis in the presence of saliva, developed to mimic the microenvironment of the oral cavity. We sought to determine its efficiency to combat oral hygiene-related diseases without affecting eukaryotic cells. Cytotoxicity, reactive oxygen species production, apoptosis induction, nanoparticles uptake, and lysosome and autophagosome metabolism were evaluated. In vitro results show that Chitlac-nAg does not exert cytotoxic effects on human gingival fibroblasts, which seem to survive through a homoeostasis mechanism involving autophagy. That suggests that the novel biomaterial Chitlac-nAg could be a promising tool in the field of dentistry.     

Multiple instance learning for classifying students in learning management systems

In this paper, a new approach based on multiple instance learning is proposed to predict student’s performance and to improve the obtained results using a classical single instance learning. Multiple instance learning provides a more suitable and optimized representation that is adapted to available information of each student and course eliminating the missing values that make difficult to find efficient solutions when traditional supervised learning is used. To check the efficiency of the new proposed representation, the most popular techniques of traditional supervised learning based on single instances are compared to those based on multiple instance learning. Computational experiments show that when the problem is regarded as a multiple instance one, performance is significantly better and the weaknesses of single-instance representation are overcome.     

On the convergence of a new time window discretization method for the traveling salesman problem with time window constraints

In this paper, we discuss convergence of a time window discretization method for the traveling salesman problem with time window constraints. This recently proposed discretization method leads to a lower bound to the minimization problem. Analysis shows that this method always guarantees convergence to the optimal solution. We also illustrate that the traditional method that gives feasible solutions does not always provide such a guarantee.     

LncRNA H19 Impairs Chemo and Radiotherapy in Tumorigenesis

Various treatments based on drug administration and radiotherapy have been devoted to preventing, palliating, and defeating cancer, showing high efficiency against the progression of this disease. Recently, in this process, malignant cells have been found which are capable of triggering specific molecular mechanisms against current treatments, with negative consequences in the prognosis of the disease. It is therefore fundamental to understand the underlying mechanisms, including the genes—and their signaling pathway regulators—involved in the process, in order to fight tumor cells. Long non-coding RNAs, H19 in particular, have been revealed as powerful protective factors in various types of cancer. However, they have also evidenced their oncogenic role in multiple carcinomas, enhancing tumor cell proliferation, migration, and invasion. In this review, we analyze the role of lncRNA H19 impairing chemo and radiotherapy in tumorigenesis, including breast cancer, lung adenocarcinoma, glioma, and colorectal carcinoma.     

Analysis of Water-Soluble Vitamins in Honey by Isocratic RP-HPLC

An isocratic RP-HPLC method has been developed for the identification and quantification of water-soluble vitamins in honey. The mobile phase tested was an aqueous solution of sulphuric acid and the detection was carried out simultaneously by UV and fluorescence. The separation of vitamins C (l-ascorbic acid), B₁ (thiamine), B₃N (nicotinamide), B₃H (nicotinic acid), B₅ (d-pantothenic acid) and B₆ (pyridoxine) is achieved in these conditions in 15 min. The addition to the mobile phase of methanol 2 %?v/v reduced significantly the analysis time in the separation of these vitamins up to 10 min. Moreover, in presence of a cationic surfactant hexadecyltrimethylammonium bromide (CTAB) in the mobile phase, the separation of vitamin C, B₁, B₃N, B₃H, B₂ (riboflavin) and B₆ is possible in 6 min. The combination of both mobile phases, H₂SO₄/methanol and H₂SO₄/methanol/CTAB, has been applied to the analysis, in isocratic mode, of several monofloral honeys (rosemary, thyme, lavender, chestnut, echium) and a honeydew honey in a short time analysis.     

Rheological characterisation of cake batters generated by planetary mixing: Comparison between untreated and heat-treated wheat flours

The rheological behaviour of high ratio cake batters prepared with untreated and heat-treated wheat flours was analysed at different stages of the manufacturing process, namely slurry, (on aeration) foams and (with fat addition) aerated emulsions, featuring air volume fractions up to 0.50. Both steady shear and viscoelastic behaviours were studied. All materials exhibited shear-thinning behaviour at 20 °C over the shear rate range studied (0.07-10 s⁻¹). The generalised Cox-Merz rule could be applied to all samples. Materials prepared with heat-treated flours exhibited greater stability, as indicated by slurry thixotropy and cohesive energy, and the change in apparent viscosity and air content of foams and aerated emulsions on extended mixing. Foams and aerated emulsions showed significant elastic behaviour with G′∼G″. The temperature dependency of aerated emulsions was studied by oscillatory shear testing from 20 to 100 °C and indicated three regimes in temperature dependence: below 40 °C G′and G″ were insensitive to temperature; between 40 and 70 °C the complex viscosity exhibited Arrhenius-type behaviour, while above 70 °C G′ and G″ increased as expected for gelatinisation and foam setting. The weak gel model for foods was used to analyse the latter data sets and confirmed that the gel network generated in aerated emulsions prepared with heat-treated flours was significantly stronger than those made with unheated flours. The differences between flour types were also observed in tests on un- and heat-treated flours obtained from a second and third harvest. The impact of these quantifiable differences in rheology on performance during baking is discussed.