Hydrolysis of 4-methylumbelliferyl butyrate: A convenient and sensitive fluorescent assay for lipase activity

A rapid, sensitive and convenient fluorescent assay was developed to screen for lipase activity. The non-fluorescent substrate, 4-methylumbelliferyl butyrate (4-MUB), solubilized in either liposomal dispersions or bile salt/lecithin mixed micelles, is hydrolyzed to butyric acid and the highly fluorescent compound, 4-methylumbelliferone (4-MU). Assays are run at 37 C for 10 min, terminated, and the changes in fluorescence quantitated with a Turner III fluorometer. Both lingual and pancreatic lipases exhibit activity against this artificial substrate. The assay has several advantages: nmoles 4 MU/ml/hr are measured allowing the detection of very low lipolytic activity; multiple samples may be simultaneously assayed, and only a brief incubation period is required.     

The seismic kernel system — a large-scale exercise in fortran 77 portability

The portability of software has become a major commercial issue in recent times. Such portability does not come easily, as few if any computer languages are really portable in practice. An additional complicating factor, especially in the commercial environment, is that the resulting software must be efficient. This paper describes the design and successful implementation of a 500,000+ line portable FORTRAN 77 package for the processing of seismic data. The package exhibits demonstrably high efficiency on a wide variety of machines from minicomputers to the largest supercomputers. Experiences gained during this exercise throw much light on the integration of the various thought processes which occur during the software engineering cycle, especially the notion of locality.     

Solidification at the Micro‐Scale

The experimental determination and computer simulation of the micro‐segregation accompanying the solidification of alloys continues to be a subject of much academic and industrial interest. Both are subject to progressively more sophisticated analyses, and a discussion is offered regarding the development and practical use of such studies. Simple steels are particularly difficult targets for such work: solidification does not end conveniently in a eutectic, the rapid diffusion particularly in the delta‐ferrite phase obscures most evidence of what had occurred at the micro‐scale during solidification, and one or more subsequent solid state phase transformations further obscure such details. Also, solidification at the micro‐scale is inherently variable: the usual, dendrite morphologies encountered are, after all, instabilities in growth behaviour, and therefore such variability should be expected. For questions such as the relative susceptibility of different grades to particular problems, it is the average, typical behaviour that is of interest, whereas for other questions such as the on‐set of macro‐segregation, the local variability is paramount. Depending on the question being asked, and indeed the accuracy with which validatory data are available, simple pseudo‐analytical equations employing various limiting assumptions, or sophisticated models which remove the need for most such limitations, could be appropriate. This paper highlights the contribution to such studies of various collaborative research forums with‐in the European Union with which the author is involved.     

State psychological association creates a postdoctoral residency and internship training program.

State psychological associations can respond to supply and demand problems for postdoctoral residencies and internships by creating statewide training programs. A state psychological association training model is presented. The Arizona Psychological Association (AzPA) created a nonprofit subsidiary corporation- the Arizona Psychology Training Consortium-to increase the number of high-quality training opportunities in Arizona. The structure and operation of the program are described. The training model emphasizes cultural diversity, psychotherapy, and independent practice. Members of the practice community in Arizona serve as supervisors, trainers, and mentors. From 2001 through 2006, 19 postdoctoral residents and 51 interns completed the program. AzPA has benefited from increased student involvement. Many residents and interns later became association members. Arizona can serve as a model for other states that wish to help their professionals in training. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

2DEG base hot electron transistors fabricated using MBE and in situion beam lithography

A vertical hot electron transistor incorporating a two-dimensional electron gas (2DEG) base has been fabricated in the GaAs-AlGaAs materials system. The difficulties caused by the need to form selective ohmic contacts to the different conducting layers have been overcome using a combination of in situ focused ion beam (FIB) isolation and molecular beam epitaxial (MBE) regrowth. This has allowed a high yield of working devices to be achieved with a typical common emitter current gain of h_FE=6 at low temperatures     

Structural transformations in surface layers of amorphous ribbons Ni78SixB22−x

The thermal stability of NI₇₈Si_xB_22–x (x = 6, 9, 12, 15) metallic glasses, produced by the single-roll melt spinning method, has been investigated by differential thermal analysis (DTA) and by measuring the temperature dependences of the exoelectron emission (EEE) and contact potential difference (CPD) relative to platinum. It has been found that both CPD and EEE show anomalies at temperatures corresponding to the structural transformations in the surface layer of investigated materials. The surface crystallization occurs at temperature of 150 K lower than the volume crystallization. The activation energy for the surface crystallization is also distinctly lower than that for crystallization in bulk.     

Types and associated type families for hardware simulation and synthesis

In this article we overview the design and implementation of the second generation of Kansas Lava. Driven by the needs and experiences of implementing telemetry decoders and other circuits, we have made a number of improvements to both the external API and the internal representations used. We have retained our dual shallow/deep representation of signals in general, but now have a number of externally visible abstractions for combinatorial and sequential circuits, and enabled signals. We introduce these abstractions, as well as our abstractions for reading and writing memory. Internally, we found the need to represent unknown values inside our circuits, so we made aggressive use of associated type families to lift our values to allow unknowns, in a principled and regular way. We discuss this design decision, how it unfortunately complicates the internals of Kansas Lava, and how we mitigate this complexity. Finally, when connecting Kansas Lava to the real world, the standardized idiom of using named input and output ports is provided by Kansas Lava using a new monad, called Fabric. We present the design of this Fabric monad, and illustrate its use in a small but complete example.