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R Romeo V Thiers F Driss P Berthelot B Nalpas C Brechot 《Canadian Metallurgical Quarterly》1993,33(8):629-633
The pattern of hepatitis C virus (HCV) viremia in blood donors who are positive for antibody to HCV (anti-HCV) according to the level of transaminase activity is unclear. A polymerase chain reaction-based HCV RNA detection method was used to study two clearly defined groups of anti-HCV-positive blood donors with repeatedly normal (n = 27) and elevated (n = 17) alanine aminotransferase (ALT) levels. HCV RNA was detected in only 4 of 27 blood donors with normal ALT values and 15 of 17 with elevated ALT values. These results indicate that anti-HCV-positive blood donors with normal ALT levels constitute a heterogeneous group, as HCV viremia is detectable in only a small proportion of cases. Polymerase chain reaction should be useful in the surveillance of anti-HCV-positive blood donors with normal ALT levels, by identifying those who might benefit from further investigation and treatment. 相似文献
3.
A genetic algorithm for the optimisation of assembly sequences 总被引:6,自引:0,他引:6
This paper describes a Genetic Algorithm (GA) designed to optimise the Assembly Sequence Planning Problem (ASPP), an extremely diverse, large scale and highly constrained combinatorial problem. The modelling of the ASPP problem, which has to be able to encode any industrial-size product with realistic constraints, and the GA have been designed to accommodate any type of assembly plan and component. A number of specific modelling issues necessary for understanding the manner in which the algorithm works and how it relates to real-life problems, are succinctly presented, as they have to be taken into account/adapted/solved prior to Solving and Optimising (S/O) the problem. The GA has a classical structure but modified genetic operators, to avoid the combinatorial explosion. It works only with feasible assembly sequences and has the ability to search the entire solution space of full-scale, unabridged problems of industrial size. A case study illustrates the application of the proposed GA for a 25-components product. 相似文献
4.
Amati Viviana; Werge Thomas M.; Cattaneo Antonino; Tramontane Anna 《Protein engineering, design & selection : PEDS》1995,8(4):403-408
Substance P G-protein coupled receptor and the antigen recognitionsite of a monoclonal antibody raised against substance P sharea stretch of five contiguous identical amino acids. This observationprompted us to build an atomic model of both the receptor andthe antibody and to analyse their common features. In particular,we report here that a pocket of similar size and compositionis present in both proteins, strongly suggesting a similarityin the mode of binding of both macromolecules to substance P.From the analysis of our models, the available data on the modeof binding of the antibody to substance P and recent data onsubstance P receptor mutants, we concluded that the pocket isvery likely to be involved in binding of the C-terminal 'messagesequence' of the tachykinin. This allowed us to suggest specificsite-directed mutants of the receptor which should shed somelight on the mechanism of peptide recognition by G-protein coupledreceptors. 相似文献
5.
PD Miles S Li M Hart O Romeo J Cheng A Cohen K Raafat AR Moossa JM Olefsky 《Canadian Metallurgical Quarterly》1998,101(1):202-211
The aim of this work was to identify which proteins in horse dander extracts are allergens and to characterise them. Two-dimensional PAGE showed that horse dander preparations are composed of up to 50 proteins, all having acidic isoelectric points in the pH range 3-4.5. Immunoblots of two-dimensional PAGE were used to compare the reactivity of the proteins with IgE from 23 allergic patients. Patient sera were divided into two main groups recognising either allergens of 18.5 kDa or proteins of 27-29 and 31 kDa. The proteins of 27-29 kDa and 31 kDa were all N-glycosylated and their glycan chains seem to play a role in the binding of IgE from allergic patients. The sugar composition of their carbohydrate moiety was determined and lectin-binding experiments indicated presence of terminal sialic acid linked alpha-(2-->6) to galactose, galactose linked beta-(1-->4) to N-acetylglucosamine, and possibly presence of sialic acid linked alpha-(2-->3) to galactose. The 27-29-kDa glycoproteins had heterogeneous isoelectric points, most probably due to different degrees of sialylation in their oligosaccharide chains. The two 18.5-kDa allergens exhibited slightly different isoelectric points and their N-terminal sequences were identical, showing that they most likely were isoforms of the same protein. Sequence analyses revealed that their N-terminal sequences are similar to proteins belonging to the lipocalin family. We named the two 18.5-kDa proteins Equ c 2.0101 and Equ c 2.0102, according to International Allergen Nomenclature recommendations [King, T. P., Hoffman, D., Lowenstein, H., Marsh, D. G., Platts-Mills, T. A. E. & Thomas, W. (1995) J. Allergy Clin. Immunol. 96, 5-14]. The N-terminal of the allergens of 27-29 kDa were blocked and their sequences were not determined. Their amino acid compositions were determined and comparison with acidic mammalian proteins in the Swiss-Prot database revealed high scores with lipocalin proteins. This suggests that the glycosylated horse dander allergens belong to the lipocalin family, like Equ c 2.0101 and Equ c 2.0102. 相似文献
6.
In this short paper we present a discrete-time field oriented controller (FOC) for current-fed induction motors which insures global asymptotic speed regulation as well as rotor flux norm tracking. To the best of our knowledge, this is the first time such a result is rigorously established for a controller implemented in discrete-time. To insure global stability a condition on the reference for the rotor flux norm, which is time-varying, is imposed. This condition disappears as the sampling period goes to zero, hence allowing for independent speed regulation and rotor flux norm tracking. One important feature of our scheme is that, compared with the first-difference approximation of the classical indirect FOC, the additional computational burden is negligible. It is also shown that the result can easily be extended to the case of tracking time-varying references in speed or position. 相似文献
7.
Polygonella myriophylla is a perennial shrub endemic to the Florida scrub. Striking bare zones surround maturePolygonella stands. Quantitative measurements of root distribution show that fewPolygonella roots extend into the bare zones, supporting the hypothesis that the bare zones result from chemical interference byPolygonella with the growth of other species. Bioassays of soils collected biweekly for one year from beneathPolygonella, the bare zone, and adjacent grassed areas confirm that the germination and growth of grasses is reduced inPolygonella soil and soil from the surrounding bare zone. Compared to adjacent grassed area soil, the average germination of bahiagrass (Paspalum notatum) was 71% inPolygonella soil and 81% in bare zone soil, and average shoot dry weight was 48% inPolygonella soil and 81% in bare zone soil. Seasonal variation in the inhibition of grass germination and growth was not pronounced. 相似文献
8.
The insecticidal nonprotein amino acid S-(-carboxyethyl)-cysteine (S-CEC) is the major free amino acid in the seeds of severalCalliandra spp. where it accounts for up to 2.9 % of dry weight. Lesser amounts of other related S-containing amino acids and an array of nonprotein imino acids derived from pipecolic acid are other constituents. High concentrations of imino acids, which also show insecticidal activity, are maintained in the mature leaves, but sulfur compounds are lacking. In this study the disappearance of S-CEC from the germinating seeds and young seedlings ofC. rubescens was monitored over time. After 10 weeks, S-CEC continues to be found in high concentrations in the stems and new leaves. As young leaves mature, sulfur compounds quickly decrease in concentration. Traces of S-CEC are found in new leaves of plants up to nine months after germination. Whether high concentration of S-CEC in young leaves is due to transportation from the seed or de novo synthesis is unclear. The ecological implications are discussed. 相似文献
9.
In this paper the effect of temperature and filler content ? on electrical resistivity of styrene-acrylonitrile polymer filled with iron or aluminium powders has been investigated. The resistivity of such composites decreases suddenly by several orders of magnitude at a critical volume concentration ?c, which depends on the size distribution of particles and on thermal history. For filler content lower than ?c, the resistivity decreases with increasing temperature, whereas for ? > ?c there is a sudden increase in resistivity at temperatures near the glass transition temperature of the polymeric matrix. This effect is more pronounced than the previously reported data on other composite systems. 相似文献
10.