Net N immobilisation during the biodegradation of mucilage in soil as affected by repeated mineral and organic fertilisation

Repeated fertilisation of crops with manure commonly increases both the amount and the quality of soil organic matter, the mineralisation of which strongly determines the availability of N to plants. In the rhizosphere, the microbial mineralisation/immobilisation of N is enhanced due to rhizodeposition (release of organic compounds from roots). In this work, we studied N transformations during incubation of maize root mucilage in soils that had been previously fertilized with composted pig manure or with ammonium nitrate for 7 years. Our work revealed mucilage was rapidly mineralised (average half-life of 3 days), inducing a rapid N immobilisation of 94 mg N g⁻¹ of mucilage C, followed by a slow remineralisation. Fertilisation with manure induced a persistent stimulation of the soil organic matter mineralisation, leading to an enhanced content of soil inorganic N (+23% in 58 days of incubation). Due to this stimulation of microbial activity in the soil fertilized with manure, the decrease in inorganic N during the biodegradation of mucilage lasted a shorter time as compared to the mineral fertilisation. However, the type of fertilisation did not significantly change the amount of N immobilised.     

Understanding the Interactions of Guanine Quadruplexes with Peptides as Novel Strategies for Diagnosis or Tuning Biological Functions

Guanine quadruplexes (G4s) are nucleic acid structures exhibiting a complex structural behavior and exerting crucial biological functions in both cells and viruses. The specific interactions of peptides with G4s, as well as an understanding of the factors driving the specific recognition are important for the rational design of both therapeutic and diagnostic agents. In this review, we examine the most important studies dealing with the interactions between G4s and peptides, highlighting the strengths and limitations of current analytic approaches. We also show how the combined use of high-level molecular simulation techniques and experimental spectroscopy is the best avenue to design specifically tuned and selective peptides, thus leading to the control of important biological functions.