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Boron nitride (BN) coatings were deposited onto die steel and graphite substrates with a low pressure r.f. plasma. The coatings were deposited onto substrates at temperatures of 550–620 °C from a gas mixture of argon, NH3 and BCl3. X-ray diffraction and scanning electron microscopy were employed to identify and to characterize the coatings. The coatings are mostly amorphous; however, the existence of small amounts of hexagonal BN was identified. The influence on the growth rate of the deposition time and the pressure in the reactor is described. 相似文献
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Bhupendar S Khatkar J David Schofield 《Journal of the science of food and agriculture》2002,82(8):827-829
Controlled stress rheometry was used to investigate the effects of starch and gluten fractions on the non‐linearity of wheat flour dough. Flour–water dough showed non‐linear viscoelastic behaviour over all stress values in a cyclic stress sweep. The amplitude‐dependent behaviour of the starch and amplitude‐independent nature of the gluten revealed that starch is responsible for the non‐linearity of the flour–water dough system. Adding starch to gluten caused a substantial narrowing of its linear viscoelastic range. © 2002 Society of Chemical Industry 相似文献
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Bhupendar S Khatkar 《Journal of the science of food and agriculture》2005,85(2):337-341
Controlled stress rheometry differentiated glutens from the cultivars under study into ‘extra‐strong’, strong and weak on the basis of their elastic and viscous moduli measured in the linear domain. The mechanical spectra of different glutens revealed no qualitative differences, but exhibited large quantitative differences in the magnitude of the dynamic measurements, ie elastic modulus, G′, viscous modulus, G″, and loss tangent, tanδ. Both covalent and non‐covalent interactions appeared to contribute to these differences. However, disulfide cross‐links proved to be especially important determinants of differences in the elastic characteristics of the glutens. The present study indicated that dynamic rheological parameters of glutens were related to their bread‐making performance, as evidenced by the significant correlations between the dynamic moduli of the glutens and loaf volume. Copyright © 2004 Society of Chemical Industry 相似文献
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A low pressure r.f. plasma was applied to deposit SiC coatings onto Ti6A14V substrates. The coatings were deposited onto substrates at temperatures of 140–390°C from a gas mixture of tetramethylsilane (TMS), argon and hydrogen. Scanning electron microscopy, X-ray diffraction and transmission electron microscopy were employed to identify and to characterize the coatings obtained. It was found that the coatings were hexagonal α-SiC of type III. The coating thickness approximately follows a parabolic time law. A maximum rate of deposition was observed in the pressure range 5–6 mbar. The rate of deposition increases with concentration of TMS up to 0.05% and remains approximately constant up to 0.12% 相似文献
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Structure–Activity Relationships of Benzenesulfonamide‐Based Inhibitors towards Carbonic Anhydrase Isoform Specificity
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Avni Bhatt Dr. Brian P. Mahon Vinicius Wilian D. Cruzeiro Dr. Benedetta Cornelio Dr. Marie Laronze‐Cochard Dr. Mariangela Ceruso Prof. Dr. Janos Sapi Dr. Graham A. Rance Prof. Dr. Andrei N. Khlobystov Assoc. Prof. Dr. Antonella Fontana Prof. Dr. Adrian Roitberg Prof. Dr. Claudiu T. Supuran Prof. Dr. Robert McKenna 《Chembiochem : a European journal of chemical biology》2017,18(2):213-222
Carbonic anhydrases (CAs) are implicated in a wide range of diseases, including the upregulation of isoforms CA IX and XII in many aggressive cancers. However, effective inhibition of disease‐implicated CAs should minimally affect the ubiquitously expressed isoforms, including CA I and II, to improve directed distribution of the inhibitors to the cancer‐associated isoforms and reduce side effects. Four benzenesulfonamide‐based inhibitors were synthesized by using the tail approach and displayed nanomolar affinities for several CA isoforms. The crystal structures of the inhibitors bound to a CA IX mimic and CA II are presented. Further in silico modeling was performed with the inhibitors docked into CA I and XII to identify residues that contributed to or hindered their binding interactions. These structural studies demonstrated that active‐site residues lining the hydrophobic pocket, especially positions 92 and 131, dictate the positional binding and affinity of inhibitors, whereas the tail groups modulate CA isoform specificity. Geometry optimizations were performed on each ligand in the crystal structures and showed that the energetic penalties of the inhibitor conformations were negligible compared to the gains from active‐site interactions. These studies further our understanding of obtaining isoform specificity when designing small molecule CA inhibitors. 相似文献
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Sena Cans?z Can ?zen Ceren Bayra? A. Tahir Bayra? Fatma Gül Murat Kavruk Remziye Y?lmaz Füsun Eyidogan Hüseyin Avni ?ktem 《European Food Research and Technology》2012,235(3):429-437
Over the last decade, array detection has been developed to qualitatively assess the presence of genetically modified organisms (GMOs). To date, DNA array systems have the highest capabilities as a result of GMOs analysis. We describe the construction of an array platform in the sandwich hybridization format for the detection of transgenic promoter of Cauliflower mosaic virus (CaMV; p35S). Sequence-specific signal development has been achieved by a sandwich complex composed of a surface immobilized capture probe and a fluorescein-tagged signal probe, which are partially complementary to the p35S oligonucleotide. We used poly-l-lysine-coated glass slides as support material, on which capture probes were immobilized by a heterobifunctional cross-linker. The comparative results of optimization studies including cross-linker types probe concentrations and hybridization conditions (sequence, temperature and duration) were reported. An optimum hybridization signal was obtained with a 32.5 ? cross-linker, 10???M capture and 20???M signal probe concentrations, respectively. A relatively short hybridization time (2.5?h) provided reproducible array signals. No significant effect of hybridization sequence on the fluorescence intensity was observed. The described platform can specifically detect label-free transgenic sequences with a target of 0.01???M concentration, while the optimized system exhibits great potential for the application of different GMO target sequences (p35S, tNOS, bar and cry) to multiplex array formats. 相似文献