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1.
Effects of passive smoking on the regulation of rat aortic cholesteryl ester hydrolases by signal transduction 总被引:2,自引:0,他引:2
The effects of exogenous oxidative stress due to passive smoking on cholesteryl ester (CE)-metabolizing enzymes and their
regulatory kinases were examined by exposing rats to cigarette smoke (CS) for a 1-h period twice a day for 8, 12, or 20 wk.
An oxidatively modified low density lipoprotein (Ox-LDL) with a high lipid peroxide was identified in three CS groups after
all three exposure periods. The rat aortic acid and neutral CE hydrolases (ACEH and NCEH) were activated to similar extents
by both cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) in the presence of their respective cofactors. The
aortic PKC activity in the three CS groups exhibited significant reductions of 72, 84, and 75% as compared with the respective
controls, which coincided with the reductions in the ACEH activities (86, 71, and 80%, respectively), whereas the PKA activities
increased to 121, 197, and 252% in the three CS groups, respectively. Reflecting the increase of the PKA activity, the NCEH
activity exhibited increases of 112% at 8 wk and 140% until 12 wk of exposure and decreased by 50% of the control value at
20 wk of exposure, suggesting inactivation of NCEH itself. The activation of acyl-CoA:cholesterol O-acyl-transferase activity was associated with an increase of free cholesterol in aorta. The vitamin E diet prevented the
formation of Ox-LDL and the oxidative inactivation of most enzymes, especially PKC, until 12 wk, but was less effective by
20 wk. The oxidative inactivation of PKC, particularly its activated form that translocated to the membrane fraction, was
confirmed in the in vitro exposure to active oxygen generators at an optimal concentration; this inactivation was prevented by catalase and superoxide
dismutase. These results suggested that the formation of Ox-LDL and alterations in CE-metabolizing enzymes caused by passive
smoking could contribute to a twofold increase in the aortic CE content, thereby contributing to one of the mechanisms for
atherosclerosis associated with smoking. 相似文献
2.
Characterization of alternative plasticizers in poly(vinyl chloride) sheets for blood containers 下载免费PDF全文
Yuji Haishima Tsuyoshi Kawakami Chie Fukui Akito Tanoue Toshiyasu Yuba Satoru Ozono Hidehumi Kumada Kaoru Inoue Tomomi Morikawa Miwa Takahashi Ayano Fujisawa Kayo Yamasaki Yusuke Nomura Kazuo Isama Ung‐il Chung Kumiko Ogawa Shingo Niimi Midori Yoshida 《乙烯基与添加剂工艺杂志》2016,22(4):520-528
This study aimed to optimize the ratio of dioctyl 4‐cyclohexene‐1,2‐dicarboxylate (DOTH) and di‐isononyl‐cyclohexane‐1,2‐dicarboxylate (DINCH®) for use as plasticizers in poly(vinyl chloride) (PVC) sheets. We also evaluated the biological safety of DOTH for its potential to be part of a safe PVC‐based blood container. The suppression of hemolysis in mannitol‐adenine‐phosphate / red cell concentrates (MAP/RCC) with DOTH/(DINCH®‐PVC) sheets and the elution of plasticizers from the sheets increased with higher DOTH compositions. The properties of the PVC sheet containing DOTH and DINCH® in the ratio of 25:33 parts against PVC 100 parts as a weight were almost identical to the PVC sheet made of di(2‐ethylhexyl) phthalate. From a subchronic toxicity test, DOTH did not show any adverse effects on all organs, including the testes, epididymis, liver, and kidneys. The no‐observed‐adverse‐effect level was 300 mg/kg body weight/day in a rat. These results suggest that DOTH/DINCH® (25:33) is a promising candidate for the replacement of di(2‐ethylhexyl) phthalate in blood containers. J. VINYL ADDIT. TECHNOL., 22:520–528, 2016. © 2015 Society of Plastics Engineers 相似文献
3.
Nariko Hosomi Ayano Tanabe Mitsunori Hieda Masaru Suzuki 《Journal of Low Temperature Physics》2005,138(1-2):361-366
No Heading We measured the slippage of 4He films adsorbed on Grafoil using the quartz-crystal microbalance (QCM) technique. The slippage of 4He films depended on the oscillating amplitude. In a large oscillating amplitude of a 5.0 MHz quartz crystal, 4He films underwent slipping gradually below a certain temperature. On the other hand, in a small oscillating amplitude, another additional increase appeared.PACS numbers: 67.20. +k, 81.40.Pq 相似文献
4.
Ayano Nakamura Susumu Suzuki Jo Kanasugi Masayuki Ejiri Ichiro Hanamura Ryuzo Ueda Masao Seto Akiyoshi Takami 《International journal of molecular sciences》2021,22(19)
The prognosis of multiple myeloma (MM) has drastically improved owing to the development of new drugs, such as proteasome inhibitors and immunomodulatory drugs. Nevertheless, MM is an extremely challenging disease, and many patients are still refractory to the existing therapies, thus requiring new treatment alternatives. Venetoclax is a selective, orally bioavailable inhibitor of BCL-2 that shows efficacy in MM not only as a single agent but also in combination therapy, especially for MM patients with translocation t(11;14). However, many patients are refractory to this drug. Here, we treated the MM cell lines KMS12PE and KMS27 with a combination treatment of venetoclax targeting BCL-2 and daratumumab targeting CD38 to evaluate the synergistic cytotoxicity of these drugs in vitro. MM cell lines were co-cultured with natural killer (NK) cells at an effector:target ratio of 0.3:1 in the presence of serial concentrations of daratumumab and venetoclax, and the resulting apoptotic MM cells were detected by flow cytometry using annexin V. These results indicated that the antibody-dependent cell-mediated NK cytotoxicity was enhanced in KMS12PE and KMS27 cells harboring t(11;14) with a high BCL-2 expression, suggesting that the combination treatment of venetoclax and daratumumab should be especially effective in patients with these characteristics. 相似文献
5.
Milk‐derived peptide Val‐Pro‐Pro (VPP) inhibits obesity‐induced adipose inflammation via an angiotensin‐converting enzyme (ACE) dependent cascade 下载免费PDF全文
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A method of image encryption and decryption is proposed for optical security systems. A phase-coded image to be encrypted together with a random phase pattern is Fourier transformed and the result of the complex-valued data is used as an encrypted pattern. The decryption is simply performed by an inverse-Fourier transform for the addition of the encrypted pattern and the Fourier transform of the random phase. The intensity of the inverse-Fourier transformed image gives the exact result forthe decryption. Further, the binarization of the encrypted pattern is performed for the easiness of the optical and electronic readout of the images, and it also gives rise to the enhancement of the degree of security for the encryption. The binary pattern is optimized by a statistical iteration technique and an excellent decryption image is obtained by the optimization. 相似文献
8.
Eiko Sakurai Hisato Ishizawa Yuka Kiriyama Ayano Michiba Yasushi Hoshikawa Tetsuya Tsukamoto 《International journal of molecular sciences》2022,23(12)
In recent years, the choice of immune checkpoint inhibitors (ICIs) as a treatment based on high expression of programmed death-ligand 1 (PD-L1) in lung cancers has been increasing in prevalence. The high expression of PD-L1 could be a predictor of ICI efficacy as well as high tumor mutation burden (TMB), which is determined using next-generation sequencing (NGS). However, a great deal of effort is required to perform NGS to determine TMB. The present study focused on γH2AX, a double-strand DNA break marker, and the suspected positive relation between TMB and γH2AX was investigated. We assessed the possibility of γH2AX being an alternative marker of TMB or PD-L1. One hundred formalin-fixed, paraffin-embedded specimens of lung cancer were examined. All of the patients in the study received thoracic surgery, having been diagnosed with lung adenocarcinoma or squamous cell carcinoma. The expressions of γH2AX and PD-L1 (clone: SP142) were evaluated immunohistochemically. Other immunohistochemical indicators, p53 and Ki-67, were also used to estimate the relationships of γH2AX. Positive relationships between γH2AX and PD-L1 were proven, especially in lung adenocarcinoma. Tobacco consumption was associated with higher expression of γH2AX, PD-L1, Ki-67, and p53. In conclusion, the immunoexpression of γH2AX could be a predictor for the adaptation of ICIs as well of as PD-L1 and TMB. 相似文献
9.
Ayano Kikuchi Satoshi Sugiyama Toshiya Nakaguchi Satoshi Yamamoto Takeshi Oji Hirobumi Shimada Yuji Kasahara Takao Namiki Koutaro Yokote Norimichi Tsumura Yoichi Miyake 《Artificial Life and Robotics》2014,19(1):1-8
Since metabolic syndrome is a cause of lifestyle-related diseases, and its early diagnosis and an evaluation of visceral fat are important, this study proposes a new method of estimating the amount of fat. The goal of this study is to estimate visceral fat from estimation of subcutaneous fat and total fat. The subcutaneous fat is estimated from the elasticity of the lumbar area, and the total fat is estimated from body information such as the abdominal vertical and lateral width, and body weight. Finally, the estimated subcutaneous fat is subtracted from the estimated total fat to calculate the visceral fat. Measurement tests conducted to verify the constructed visceral fat estimation model showed good results, with a correlation of 0.96 between the visceral fat area estimated by the proposed method and the actual measured area. The reproducibility of the estimation model was also verified by the cross validation method. 相似文献
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