Electrospun fibers based on porcine plasma: a rheological and morphological study

Iranian Polymer Journal - The present work focuses on the assessment of the ability of porcine plasma protein (PPP) to be electrospun satisfactorily to form fibre mats, and their rheological and...     

Learning teamwork skills in university programming courses

University courses about computer programming usually seek to provide students not only with technical knowledge, but also with the skills required to work in real-life software projects. Nowadays, the development of software applications requires the coordinated efforts of the members of one or more teams. Therefore, it is important for software professionals to master the sort of skills that assure the success of teamwork, such as communication, leadership, negotiation, or team management. However, these abilities are difficult to teach, one of the reasons being that they require true commitment from the students. However, today students are taking a more and more passive role in their own education, two of the more evident consequences being the increase in dropout rates and the decrease in marks obtained in exams. The NUCLEO e-learning framework has been designed to promote the effective acquisition of teamwork skills and, at the same time, to promote the more active participation of the students in their own learning process. NUCLEO adopts a socio-constructivist pedagogical approach that pursues the development of communities of practice for Problem Based Learning. Our research has rooted the design decisions of NUCLEO in the analysis of its socio-cultural environment with Activity Theory, which considers conflicts within groups as the impetus of their evolution and the forges of their environments. This paper presents the analysis of the main features of NUCLEO according to Activity Theory, as well as the experimental results obtained with the framework in three different case studies in university courses.     

Phenotypic,genetic and technological characterization of Lactococcus garvieae strains isolated from a raw milk cheese

A series of Lactococcus garvieae strains isolated as the majority population of a Spanish traditional, starter-free cheese made from raw milk were phenotypically and genotypically characterised to address their biochemical potential, safety requirements, and technological properties. As expected, all L. garvieae cheese strains fermented lactose but grew slowly in UHT-treated milk. Enzymatic activities of L. garvieae were similar to those of Lactococcus lactis, although higher esterase and lipase activities were recorded for L. garvieae strains. Profiles of the volatile compounds produced from milk by L. garvieae and L. lactis strains were also comparable. L. garvieae strains did not produce haemolysin, gelatinase and the biogenic amines tyramine and histamine. Five L. garvieae stains showed tetracycline resistance encoded by a tet(M) gene. The use of L. garvieae strains as starter or adjunct cultures might be recommended for certain cheese types, provided that the safety of the strains has been demonstrated.     

Carob Fruit Polyphenols Reduce Tocopherol Loss,Triacylglycerol Polymerization and Oxidation in Heated Sunflower Oil

Heated oils may contain potentially toxic altered compounds. A denatured carob fiber, very rich in non-extractable tannins (Exxenterol^®), exhibits antioxidant activities in in vitro experiments. The present study was designed to evaluate in sunflower oil (SO) heated to frying temperature, the protective effect on oil thermal oxidation and polymerization of adding 10 mg Exxenterol/kg oil (SO-10) and 50 mg Exxenterol/kg oil (SO-50). After 2, 8 and 16 h at 180 °C, SO displayed a relevant increase in triacylglycerol-derived polar material (PM) and polymer contents and a decrease in α-tocopherol concentrations. Thermal oxidation changes were significantly checked in SO-50 throughout the 16-h heating, while SO-10 only displayed protection from thermal oxidation during the first 2 h of heating. Oil frying-life was doubled because formation of PM and polymers was inhibited by more than 50%. Results clearly show that this non-extractable tannin-rich fiber can be successfully employed as an additive to significantly prolong sunflower oil frying-life, and thus decrease the potential toxicity of the heated oil.     

Visual Zero: A persistent and interactive object-oriented programming environment

In this article, an ongoing research project held in the Computer Science department of the University of Vigo is described. Its main objective is to develop an interactive object-oriented environment, serving as a vehicle for learning object-oriented programming. It currently consists of a virtual machine, an assembler, compilers for two high-level programming languages, and an educational visual programming environment. Its main characteristics are (a) the support of prototype-based object orientation, which is a model of object orientation that actually wraps the class-based model; (b) the support for object persistence, which simplifies all input/output issues to the minimum, and (c) the availability of a new visual programming environment is an invaluable help. We the authors think that the combination of all of these possibilities, will lead to an interesting, useful tool that would be recommended for object-oriented teaching. The whole system has been successfully employed in a number of different courses, allowing students to concentrate on objects and their relationships from the very beginning, and thus helping them to achieve a high degree of knowledge about the object-oriented programming paradigm.     

Conjugated Polymers of Intrinsic Microporosity (C‐PIMs)

Conjugated microporous polymers (CMPs) have shown great potential for energy and environmental issues, however, poor solubility and processability of most of these materials limit their applications. Herein, a range of linear conjugated polymers of intrinsic microporosity (C‐PIMs) is reported, combining for the first time the properties of conjugated microporous polymers, such as tunable electronic properties and compositional variation, with those of linear polymers of intrinsic microporosity (PIMs) allowing for solution processability and film formation. These soluble materials have a number of potential applications, for example as components in devices where large, porous interfaces are combined with extended electronic conjugation.     

Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci

Specific PCR and sequencing showed that a tet(M) gene was present in two tetracycline-resistant Lactococcus lactis strains isolated from a raw milk, starter-free cheese. Hybridisation experiments using as a probe an internal segment of the gene obtained by PCR associated tet(M) with plasmids of around the same size (30 kbp) in both strains. Molecular analysis of the tetracycline resistance loci, including the upstream and downstream regions of the genes, showed them to be identical to one other and to the tet(M) encoded by the conjugative transposon Tn916. Amplification of Tn916-derived segments suggested the transposon was complete in the two L. lactis strains. Further, curing of the tetracycline resistance was accompanied by a reduction in size of the plasmids comparable to that expected for Tn916. Tetracycline resistance could be transferred by conjugation to plasmid-free Lactococcus and Enterococcus strains. However, no plasmid DNA was detected among the transconjugants while both tet(M) and transposon-related sequences were amplified by PCR. This suggested that only the transposon was mobilized.     

Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria

Resistance to antimicrobials is a growing problem of worldwide concern. Plasmids are thought to be major drivers of antibiotic resistance spread. The present work reports a simple way to recover replicative plasmids conferring antibiotic resistance from the bacteria in cheese. Purified plasmid DNA from colonies grown in the presence of tetracycline and erythromycin was introduced into plasmid-free strains of Lactococcus lactis, Lactiplantibacillus plantarum and Lacticaseibacillus casei. Following antibiotic selection, the plasmids from resistant transformants were isolated, analyzed by restriction enzyme digestion, and sequenced. Seven patterns were obtained for the tetracycline-resistant colonies, five from L. lactis, and one each from the lactobacilli strains, as well as a single digestion profile for the erythromycin-resistant transformants obtained in L. lactis. Sequence analysis respectively identified tet(S) and ermB in the tetracycline- and erythromycin-resistance plasmids from L. lactis. No dedicated resistance genes were detected in plasmids conferring tetracycline resistance to L. casei and L. plantarum. The present results highlight the usefulness of the proposed methodology for isolating functional plasmids that confer antibiotic resistance to LAB species, widen our knowledge of antibiotic resistance in the bacteria that inhabit cheese, and emphasize the leading role of plasmids in the spread of resistance genes via the food chain.     

Modularizing application and database evolution – an aspect‐oriented framework for orthogonal persistence

In the maintenance of software applications, database evolution is one common difficulty. In object‐oriented databases, this process comprises schema evolution and instance adaptation. Both tasks usually require significant effort from programmers and database administrators. In this paper, we propose orthogonal persistence and aspect‐oriented programming to support semi‐transparent database evolution. A default mechanism for instance evolution is defined, but the user may provide modularized solutions using the aspect‐oriented paradigm. We present our framework AOF4OOP to test the feasibility of our proposed approach. This prototype allows programmes to transparently access data in other versions of the database schema. We evaluate our framework, comparing it to related approaches using two real applications and measuring the improvement of the productivity of the programmer. Copyright © 2016 John Wiley & Sons, Ltd.