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Leščić Ašler I Ivić N Kovačić F Schell S Knorr J Krauss U Wilhelm S Kojić-Prodić B Jaeger KE 《Chembiochem : a European journal of chemical biology》2010,11(15):2158-2167
Several hydrolases of the SGNH superfamily, including the lipase SrLip from Streptomyces rimosus (Q93MW7), the acyl-CoA thioesterase I TesA from Pseudomonas aeruginosa (Q9HZY8) and the two lipolytic enzymes EstA (from P. aeruginosa, O33407) and EstP (from Pseudomonas putida, Q88QS0), were examined for promiscuity. These enzymes were tested against four chemically different classes of a total of 34 substrates known to be hydrolysed by esterases, thioesterases, lipases, phospholipases, Tweenases and proteases. Furthermore, they were also analysed with respect to their amino acid sequences and structural homology, and their phylogenetic relationship was determined. The Pseudomonas esterases EstA and EstP each have an N-terminal domain with catalytic activity together with a C-terminal autotransporter domain, and so the hybrid enzymes EstA(N)-EstP(C) and EstP(N)-EstA(C) were constructed by swapping the corresponding N- and C-terminal domains, and their hydrolytic activities were compared. Interestingly, substrate specificity and kinetic measurements indicated a significant influence of the autotransporter domains on the catalytic activities of these enzymes in solution. TesA, EstA and EstP were shown to function as esterases with different affinities and catalytic efficacies towards p-nitrophenyl butyrate. Of all the enzymes tested, only SrLip revealed lipase, phospholipase, esterase, thioesterase and Tweenase activities. 相似文献
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Aran Rafferty Anton Clifford Robert Hill David Wood Bisserka Samuneva Margarita Dimitrova-Lukacs 《Journal of the American Ceramic Society》2000,83(11):2833-2838
The nucleation and crystallization of a series of glasses based on 4.5SiO2 ·3Al2 O3 ·1.5P2 O5 ·(5 − z )CaO · z CaF2 with a Ca:P ratio corresponding to apatite were studied. In these glasses, the objective was to investigate the influence of fluorine content and z was varied from 3 to 0. All the glasses studied crystallized to fluorapatite (FAP) and mullite with the exception of the glass containing no fluorine, which crystallized to β-tricalcium phosphate (Ca3 (PO4 )2 ) and anorthite (CaAl2 Si2 O8 ). Glasses that contained sufficient fluorine to form FAP bulk nucleated to give FAP without a nucleation hold. Thermal gravimetric analysis demonstrated a significant weight loss corresponding to the crystallization of mullite, which increased with the fluorine content of the glass and also with decreased particle size. The loss was attributed to volatile SiF4 . The glass transition temperature decreased with increased fluorine content of the glass. 相似文献
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Ljubica Djukanović Biserka Aksić‐Miličević Miodrag Antić Jovan Baković Željko Varga Biljana Gojaković Nada Dimković Verica Ðorđević Vidojko Ðorđević Stevan Ðurić Sunčica Ðurić Nenad Zec Rosa Jelačić Zoran Kovačević Tatjana Lazarević Višnja Ležaić Milan Mandić Ivko Marić Srboljub Milenković Olivera Milićević Milena Mišković Igor Mitić Zora Nikolić Draga Pilipović Stevo Plješa Miroslava Radaković Nenad Rakić Vanja Rangelov Radivoje Stojanović Marina Stojanović‐Stanojević Biserka Tirmenštajn‐Janković Branimir Haviža‐Lilić Edvin Hadžibulić Rajko Hrvačević Anica Cvetičanin 《Hemodialysis international. International Symposium on Home Hemodialysis》2012,16(4):517-525
The study presents the epidemiological features of patients treated with renal replacement therapy (RRT) in Serbia from 1997 to 2009 and compares the results of hemodialysis treatment in 1999 and 2009. Epidemiological data were obtained from the National Registry of RRT patients and data on hemodialysis treatment from special surveys conducted in 1999 and 2009. Within the period 1997–2009 the incidence of patients on RRT increased from 108 to 179 per million population (pmp), prevalence rose from 435 to 699 pmp, while mortality rate fell from 20.7% to 16.7%. The frequency of patients with glomerulonephritis decreased, while that of patients with diabetes and hypertensive nephropathy increased. In late 2009 there were 5208 patients receiving RRT in Serbia. Within the examined period new hemodialysis and reverse osmosis equipment were purchased, high‐flux dialyzers with synthetic membranes were increasingly used and the number of patients receiving hemodiafiltration increased to 17.6%. Kt/V greater than 1.2 was recorded in 16% of the patients in 1999 but 52% in 2009. Options for correction of anemia and mineral disorders have also improved. The percentage of patients with HbsAg (13.8% vs. 4.8%) as well as anti‐hepatitis C virus antibodies positive patients (23.2% vs. 12.7%) was significantly lower in 2009 than in 1999. Both the incidence and prevalence of RRT patients in Serbia are rising continuously, while the mortality rate is falling. More favorable conditions for dialysis treatment have brought about significant improvement in the results over the last 10 years. 相似文献
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