The Impact of Selected Bacterial Sexually Transmitted Diseases on Pregnancy and Female Fertility

Sexually transmitted infections (STIs) caused by Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium are a common cause of pelvic inflammatory disease (PID) which can lead to tubal factor infertility (TFI). TFI is one of the most common causes of infertility, accounting for 30% of female fertility problems. STIs can also have an impact on pregnancy, leading to adverse pregnancy outcomes. Escalating antibiotic resistance in Neisseria gonorrhoeae and Mycoplasma genitalium represents a significant problem and can be therapeutically challenging. We present a comprehensive review of the current treatment options, as well as the molecular approach to this subject. We have given special attention to molecular epidemiology, molecular diagnostics, current and new treatments, and drug resistance.     

Laparoscopic peroperative cholangiography--indications and significance

The authors have performed 35 peroperative laparoscopic cholangiographies by means of transcystic cannylation of the choledochus in 410 patients subdued to laparoscopic cholecystectomy. Choledocholelithiasis was found in 8 patients (22.85%). The authors advocate for the application of peroperative cholangiography in indicated cases since the method is simple, safe and sufficiently successful. (Tab. 5, Ref. 9.)     

Leptin--missing link between the metabolic state and the reproductive system?

What is the signal between the metabolic state and reproductive function--it is one of the scientific puzzles in gynecological endocrinology. Previously it was suggested that such substances as insulin, amino acids, IGFBP-I may play a role as a metabolic signal. Leptin a newly discovered hormonal product of obesity (ob) gene is expressed by adipocytes and thought to play a role in the regulation of food intake, metabolism and reproduction. In this article some informations about leptin secretion, its regulation and localization of leptin receptors have been presented. Particular attention to leptin influence on GnRH secretion have been paid. Probably on the base of this mechanism leptin acts as a link between metabolic state and reproductive system. Some data about leptin secretion in obesity, weight loss related amenorrhoea and anorexia nervosa have been discussed. The putative role of leptin in the pathophysiology of polycystic ovary syndrome and initiation of puberty also have been analyzed.     

Modeling and iterative learning control of spatially distributed parameter systems with sensing and actuation over a selected area of the domain

Multidimensional Systems and Signal Processing - This paper gives new contributions to the development of iterative learning control for distributed parameter systems, based on using finite...     

On the control of distributed parameter systems using a multidimensional systems setting

The unique characteristic of a repetitive process is a series of sweeps, termed passes, through a set of dynamics defined over a finite duration with resetting before the start of the each new one. On each pass an output, termed the pass profile is produced which acts as a forcing function on, and hence contributes to, the dynamics of the next pass profile. This leads to the possibility that the output, i.e. the sequence of pass profiles, will contain oscillations which increase in amplitude in the pass-to-pass direction. Such behavior cannot be controlled by standard linear systems approach and instead they must be treated as a multidimensional system, i.e. information propagation in more than one independent direction. Physical examples of such processes include long-wall coal cutting and metal rolling. In this paper, stability analysis and control systems design algorithms are developed for a model where a plane, or rectangle, of information is propagated in the pass-to-pass direction. The possible use of these in the control of distributed parameter systems is then described using a fourth-order wavefront equation.     

Inline injection microdevice for attomole-scale sanger DNA sequencing

A new affinity-capture-based inline purification, concentration, and injection method is developed for microchip capillary electrophoresis (CE) and used to perform efficient attomole-scale Sanger DNA sequencing separations. The microdevice comprises three axial domains for nanoliter-scale sequencing sample containment, sample plug formation, and high-resolution capillary gel electrophoresis. Purified and concentrated inline sample plugs are formed by electrophoretically driving Sanger sequencing extension fragments into an affinity-capture polymer network positioned within a CE separation channel. Extension fragments selectively hybridize and concentrate at the polymer interface while residual primer, nucleotides, and salts electrophorese out of the system. The plug is thermally released and injected into the CE channel by direct application of the separation voltage. To evaluate this system, 30 nL of sequencing sample prepared from 100 amol (60 million molecules) of human mitochondrial hypervariable region II amplicon was introduced into the microchip, purified, concentrated, and injected, generating a read length of 365 bases with 99% accuracy. This efficient inline injection system obviates the need for the excess sample that is required by cross-injection techniques, thereby enabling Sanger sequencing and other high-performance genetic analysis using DNA quantities approaching theoretical detection limits.     

Photon number resolving in geiger mode avalanche photodiode photon counters

Abstract

The paper reports on research and development in the field of avalanche photodiodes operated as photon counters in a Geiger mode. A technique has been developed and tested that permits estimation of the photon number involved in a detection process. It can be applied in a time correlated photon counting experiment simultaneously with original required time interval estimation. A time walk compensation circuit provides uniform electrical pulses, and the time interval between them is related to the number of photons detected. Employing a picosecond event timing device, the photon number can be estimated within the dynamic range 1–1000 photons with resolution better than a factor of three.     

High-throughput single copy DNA amplification and cell analysis in engineered nanoliter droplets

A high-throughput single copy genetic amplification (SCGA) process is developed that utilizes a microfabricated droplet generator (microDG) to rapidly encapsulate individual DNA molecules or cells together with primer functionalized microbeads in uniform PCR mix droplets. The nanoliter volume droplets uniquely enable quantitative high-yield amplification of DNA targets suitable for long-range sequencing and genetic analysis. A hybrid glass-polydimethylsiloxane (PDMS) microdevice assembly is used to integrate a micropump into the microDG that provides uniform droplet size, controlled generation frequency, and effective bead incorporation. After bulk PCR amplification, the droplets are lysed and the beads are recovered and rapidly analyzed via flow cytometry. DNA targets ranging in size from 380 to 1139 bp at single molecule concentrations are quantitatively amplified using SCGA. Long-range sequencing results from beads each carrying approximately 100 amol of a 624 bp product demonstrate that these amplicons are competent for achieving attomole-scale Sanger sequencing from a single bead and for advancing pyrosequencing read-lengths. Successful single cell analysis of the glyceraldehyde 3 phosphate dehydrogenase (GAPDH) gene in human lymphocyte cells and of the gyr B gene in bacterial Escherichia coli K12 cells establishes that SCGA will also be valuable for performing high-throughput genetic analysis on single cells.