The effect of grazing intensity and the presence of a forage legume on nitrogen dynamics in Brachiaria pastures in the Atlantic forest region of the south of Bahia, Brazil

It has been shown that with careful grazing management and addition of Pand K, but not N, fertilisers Brachiaria pastures are ableto maintain sustainable live weight gains over many years. However, standardon-farm practice, which generally involves high stocking rates, leads after afew years to pasture decline due mainly to N deficiency for grass regrowth. Togenerate an understanding of the mechanism of pasture decline and possiblemanagement options to mitigate this process, a study was performed in theAtlantic forest region of the south of Bahia state to study the N dynamics inpastures of Brachiaria humidicola subject to threedifferent stocking rates of beef cattle, with and without the presence of theforage legume Desmodium ovalifolium. Despite the fact thatthe C:N ratio of the deposited litter was high (60 to 70) the rate ofdecomposition was very rapid (k –0.07 gg^–1 day^–1) and annual rates of Nturnover through the litter pathway were between 105 and 170 kg Nha^–1 year^–1. In the grass-onlypasturesas stocking rate increased from 2 to 3 head ha^–1, N recycledinthe litter decreased by 11%, but a further increase to 4 headha^–1 decreased N recycling by 30% suggesting thatbeyonda certain critical level higher grazing stocking rates would lead to pasturedecline if there was no N addition. High stocking rates decreased theproportionof the legume in the sward, but at all rates the concentration of N in both thegreen and dead grass in the forage on offer and in the litter was higher in themixed sward. The presence of the legume caused a decrease in the C:N ratio ofthe microbial biomass while both soil N mineralisation and nitrificationincreased. This increased rate of turnover of the microbial biomass and thecontribution of N₂ fixation to the legume resulted in largeincreasesin the N recycled via litter deposition ranging from 42 to 155 kg Nha^–1 year^–1.     

Nitrogen fixation associated with grasses and cereals: Recent progress and perspectives for the future

Over the last 20 years many new species of N₂-fixing bacteria have been discovered in association with grasses, cereals and other non-nodulating crops. Virtually all of these bacteria are microaerophylic, fixing N₂ only in the presence of low partial pressures of oxygen. Until a few years ago much attention was focussed on members the genusAzospirillum and it was assumed that N₂ fixation was restricted to the rhizosphere or rhizoplane of the host plants. Through the use of N balance and¹⁵N techniques it has been shown that in the case of lowland rice, several tropical pasture grasses and especially sugar cane, the contributions of biological N₂ fixation (BNF) are of agronomic significance.More detailed study of the N₂-fixing bacteria associated with sugar cane (Acetobacter diazotrophicus andHerbaspirillum spp.) has shown that they occur in high numbers not only in roots of this crop but also in the stems, leaves and trash but are rarely found in the soil. Some of these endophytic diazotrophs have now also been found in forage grasses, cereals, sweet potato and cassava, although evidence of significant BNF contributions is still lacking.The identification of these endophytic diazotrophs as the organisms probably responsible for the high contributions of N₂ fixation observed in sugar cane suggests that it may be possible to attain significant BNF contributions in some other gramineae and perhaps root crops.     

Use of the 15N natural abundance technique to quantify biological nitrogen fixation by woody perennials

Biological nitrogen fixation (BNF) associated with trees and shrubs plays a major role in the functioning of many ecosystems, from natural woodlands to plantations and agroforestry systems, but it is surprisingly difficult to quantify the amounts of N₂ fixed. Some of the problems involved in measuring N₂ fixation by woody perennials include: (a) diversity in occurrence, and large plant-to-plant variation in growth and nodulation status of N₂-fixing species, especially in natural ecosystems; (b) long-term, perennial nature of growth and the seasonal or year-to-year changes in patterns of N assimilation; and (c) logistical limitations of working with mature trees which are generally impossible to harvest in their entirety. The methodology which holds most promise to quantify the contributions of N₂ fixation to trees is the so-called `¹⁵N natural abundance' technique which exploits naturally occurring differences in ¹⁵N composition between plant-available N sources in the soil and that of atmospheric N₂. In this review we discuss probable explanations for the origin of the small differences in ¹⁵N abundance found in different N pools in both natural and man-made ecosystems and utilise previously published information and unpublished data to examine the potential advantages and limitations inherent in the application of the technique to study N₂ fixation by woody perennials. Calculation of the proportion of the plant N derived from atmospheric N₂ (%Ndfa) using the natural abundance procedure requires that both the ¹⁵N natural abundance of the N derived from BNF and that derived from the soil by the target N₂-fixing species be determined. It is then assumed that the ¹⁵N abundance of the N₂-fixing species reflects the relative contributions of the N derived from these two sources. The ¹⁵N abundance of the N derived from BNF (B) can vary with micro-symbiont, plant species/provenance and growth stage, all of which create considerable difficulties for its precise evaluation. If the%Ndfa is large and the ¹⁵N abundance of the N acquired from other sources is not several ¹⁵N units higher or lower than B, then this can be a major source of error. Further difficulties can arise in determining the ¹⁵N abundance of the N derived from soil (and plant litter, etc.) by the target plant as it is usually impossible to predict which, if any, non-N₂-fixing reference species will obtain N from the same N sources in the same proportions with the same temporal and spatial patterns as the N₂-fixing perennial. The compromise solution is to evaluate the ¹⁵N abundance of a diverse range of neighbouring non-N₂-fixing plants and to compare these values with that of the N₂-fixing species and the estimate of B. Only then can it be determined whether the contribution of BNF to the target species can be quantified with any degree of confidence. This review of the literature suggests that while the natural abundance technique appears to provide quantitative measures of BNF in tree plantation and agroforestry systems, particular difficulties may arise which can often limit its application in natural ecosystems.     

Nitrogen cycling in tropical grass-legume pastures managed under canopy light interception

Nutrient Cycling in Agroecosystems - In grass-legume pastures, grazing management strategies are an essential factor affecting nitrogen (N) cycling. This study assessed the impact of grazing...     

Nitrous oxide emission and ammonia volatilization induced by vinasse and N fertilizer application in a sugarcane crop at Rio de Janeiro,Brazil

In Brazilian sugarcane plantations, fertilization with vinasse, supplemented or not with mineral fertilizer, is a common practice. But little is known about the effects of this application on N losses, especially those forms of N which give rise to greenhouse gas emissions. The aim of this study was to quantify N₂O and NH₃ emissions from soil after vinasse application and urea fertilizer addition and to examine the possible impact adding vinasse before or after urea. Two experiments were conducted under greenhouse conditions and one in the field with treatments of vinasse and urea fertilizer, either alone, or in sequence. The highest proportions of N emitted as N₂O were registered in the vinasse treatment, which amounted to 15 % of the N applied in the first greenhouse experiment, and 2.5 % in the field experiment. With respect to the losses by NH₃ volatilization, urea was the only treatment where this process was significant. N₂O emission from vinasse was 2.5 %, somewhat above the default emission factor of 1 % of the IPCC. N₂O emissions from urea were also variable, but emission factors registered were still well below the default IPCC factor for organic residues. The order of addition of urea and vinasse had little effect on NH₃ volatilization in the field, but there were evidences it was important for N₂O.     

Alternatives for nitrogen nutrition of crops in tropical agriculture

The development of sustainable agricultural systems for the tropics requires among other technologies, alternatives for nitrogen fertilizers which are often limited in availability for financial reasons and also represent a major source of groundwater and air pollution. There are many new alternatives for the development of agricultural systems which make use of biological processes in soil. Biological nitrogen fixation (BNF), that is, the biological conversion of atmospheric dinitrogen into mineral N, is the most important alternative among them. Examples are given of the impact of various technologies used in Brazil. Soybean, introduced into the country 30 years ago, is now the second most important export crop, reaching 24 million tons annually with no N fertilizer application. Consequently, Brazil today is the country in the world which uses the lowest amounts of nitrogen fertilizers in relation to phosphate. Alternatives for crop rotations and pastures are also discussed. Possibilities of expanding BNF to cereals and other non-legume crops are gaining new credibility due to the identification of endophytic associations with diazotropic bacteria. The definite proof of substantial BNF in sugar cane with N balance and¹⁵N methods in certain genotypes selected under low N fertilizer applications opens up new alternatives for sustainable agriculture and will be the key to viable bio-fuel programmes.     

Litter deposition and disappearance in Brachiaria pastures in the Atlantic forest region of the South of Bahia, Brazil

Over the last 25 years more than 70 million ha of the native vegetation in Brazil have been replaced by pastures for beef production planted to grasses of the genus Brachiaria, and to a lesser extent Andropogon gayanus, both of African origin. Some years after implantation, these pastures decline in productivity, probably due to low availability of P, and immobilisation of N in the soil due to the large quantities of senescent leaves (litter) of high C:N ratio deposited on the soil surface. In this paper we report the effects of the introduction of a forage legume (Desmodium ovalifolium) and different animal stocking rates on the deposition and decomposition of plant litter in pastures of Brachiaria humidicola at a site in the coastal Atlantic forest region of the south of Bahia State (Brazil). Litter existing on the ground, and that deposited in 14-day periods, was monitored at monthly intervals during 3 years of the study. Doubling the stocking rate from 2 to 4 animals ha-1 caused a highly significant decrease in litter deposition, but the presence of the legume in the sward had little effect. Calculations made directly from the quantities of litter deposited in the 14-day periods showed that between 15 and 18 tons of litter dry matter (dm) were deposited annually, but the relatively small quantities of existing litter (annual means of 0.8 to 1.5 t dm ha-1), showed that decomposition was rapid, showing values for half life of between 22 and 33 days. This technique was assumed to underestimate true litter disappearance rates, as with such rapid decomposition a significant proportion of the litter disappeared within the 14-day collection periods. An equation was developed to correct for this loss of litter during the collection periods and corrected litter decomposition constants of 0.037 to 0.097 g g-1 day-1 were recorded resulting in half lives of between 9 and 20 days. Using these data and adding them to estimates of animal consumption the net aerial primary productivity (NAPP) of the pastures ranged from 28 to 34 t dry matter ha-1 yr-1. Experiments with litter bags, and a "covered litter" system which allowed access of soil fauna to the litter, indicated that soil faunal activity had little impact on litter disappearance and such techniques underestimated true litter decomposition by at least an order of magnitude. We suggest that this underestimation is due to the fact, that in contrast to litter bags, in the open field situation fresh litter is being added continuously. As this material consists of both easily degradable ("active") and recalcitrant fractions, the easily degradable fraction fuels an active microbial biomass which continuously degrades the less decomposable material. It is concluded that the approach used in this study gives more realistic, and much higher estimates, of net primary aerial production of tropical grasslands and pastures than techniques heretofore utilised.     

N-fertiliser application or legume integration enhances N cycling in tropical pastures

Understanding the effects of N application or the introduction of a legume on N cycling is critical for achieving productive and sustainable grassland systems. This 2-year study assessed the N cycling of three pasture treatments: (1) mixed Marandu palisadegrass (Brachiaria brizantha) and forage peanut (Arachis pintoi) without N fertiliser (GRASS?+?LEGUME); (2) monoculture Marandu palisadegrass fertilised with 150 kg N ha^?1 year^?1 (GRASS?+?N); and (3) monoculture Marandu palisadegrass without N fertiliser (GRASS). Continuous stocking was used with a target canopy height of 0.20 to 0.25 m. Litter responses, forage and N intake, N livestock excretion and N cycling were measured. Existing litter and litter deposition rate were greatest in GRASS pasture (3030 and 84.3 vs. 2140 kg ha^?1 and 64.8 kg OM ha^?1 d^?1; average of GRASS?+?N and GRASS?+?LEGUME pastures, respectively; P?<?0.10). Litter decomposition rate in GRASS pasture was smaller 30.4 and 36.0% compared to GRASS?+?N and GRASS?+?LEGUME pastures, respectively (P?<?0.10). The GRASS?+?N obtained greatest (P?<?0.10) faecal N excretion (21.7 vs. 13.8 kg N ha^?1 season^?1), and urinary N excretion (32.0 vs. 14.2 kg N ha^?1 season^?1). In the GRASS?+?N and GRASS?+?LEGUME pastures, there was a positive overall change of N in the soil–plant–animal system of 13 and 33 kg N ha^?1 year^?1, respectively. In the GRASS pasture, there was an overall negative change of N in the soil–plant–animal system of ??41 kg N ha^?1 year^?1. Nitrogen application or the integration of forage peanut in a grass pasture increased the conservation of soil N reserves.

     

Changes in soil organic carbon during 22 years of pastures,cropping or integrated crop/livestock systems in the Brazilian Cerrado

In Brazil’s central savanna region, government policy is to encourage the conversion of conventional plough tillage (PT) agriculture to no-till (NT) and raise the productivity of under-utilized pastures, including their conversion to integrated crop-livestock (ICL) systems, with the objective of increasing soil organic carbon (SOC) at the expense of atmospheric carbon dioxide. An experiment was established in 1991 by liming and fertilizing at two levels an area of native vegetation (NV). The treatments, replicated in randomized plots, included pastures, continuous cropping and ICL systems under PT or NT. The aim of this study was to quantify the SOC accumulation to 100 cm depth under these treatments over time. The high C:N ratios suggested that there was a high proportion of charcoal present in the soil. Increasing fertilizer inputs had no overall significant effect on SOC stocks. Stocks of SOC changed little under pastures. Analyses of ¹³C abundance showed that higher fertilizer inputs increased the decomposition rate of C derived from NV under pure grass pastures. Continuous cropping under NT preserved SOC and under PT there were significant losses. The highest SOC stocks were found under ILP treatments, but not all ILP treatments accumulated SOC even under NT. These results indicate that government initiatives to substitute PT with NT and to intensify beef cattle production will have only modest short-term gains in SOC accumulation.     

Estimation of the contribution of biological N2 fixation to twoPhaseolus vulgaris genotypes using simulation of plant nitrogen uptake from15N-labelled soil

A technique for the application of the¹⁵N isotope dilution technique for the quantification of plant associated biological nitrogen fixation (BNF) was tested and applied to quantify the BNF contribution to two genotypes ofPhaseolus vulgaris. The technique makes use of sequential measurements of the¹⁵N enrichment of soil mineral N, and the uptake of labelled N by the N₂-fixing plant, to simulate its uptake of soil N (the soil to plant simulation technique). The test was made with two non-N₂-fixing crops (non-nodulating beans and wheat) and two bean genotypes (PR 923450 and Puebla 152), at two levels of N fertilizer addition (10 and 40 kg N ha^–1), to compare the actual N uptake with that simulated from the soil and crop¹⁵N data. The simulation of the soil N uptake by the non-nod bean crop using this soil to plant simulation technique underestimated by 20 to 30% the true N uptake, suggesting that the mineral N extracted from soil samples taken from the 0–15cm layer had a higher¹⁵N enrichment than that N sampled by the roots of this crop. In the case of the wheat crop the simulation resulted in a much greater underestimation of actual N uptake. In general the results using this technique suggested that BNF inputs to the bean cultivars was higher than would be expected from the nodulation and acetylene reduction data, except for the early PR beans in the 40 kg N ha^–1 treatment. In this case the total N and simulated soil N accumulation were well matched suggesting no BNF inputs. An allied technique (the plant to plant simulation technique) was proposed where the¹⁵N enrichrnent of soil mineral N was simulated from the data for total N and labelled N accumulation taken from sequential harvests of either of the non-N₂ -fixing control crops. This was then utilized in combination with the labelled N uptake data of the other crop to simulate its soil N uptake. However, the results using either technique indicated that the wheat and non-nod or nodulating beans exploited pools of N in the soil with completely different¹⁵N enrichments probably due to differences in exploitation of the soil N with depth.