Bacterial pathogens sense specific cues associated with different host niches and integrate these signals to appropriately adjust the global gene expression. Bordetella pertussis is a Gram-negative, strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Though B. pertussis does not cause invasive infections, previous results indicated that this reemerging pathogen responds to blood exposure. Here, omics RNA-seq and LC–MS/MS techniques were applied to determine the blood-responsive regulon of B. pertussis. These analyses revealed that direct contact with blood rewired global gene expression profiles in B. pertussis as the expression of almost 20% of all genes was significantly modulated. However, upon loss of contact with blood, the majority of blood-specific effects vanished, with the exception of several genes encoding the T3SS-secreted substrates. For the first time, the T3SS regulator BtrA was identified in culture supernatants of B. pertussis. Furthermore, proteomic analysis identified BP2259 protein as a novel secreted T3SS substrate, which is required for T3SS functionality. Collectively, presented data indicate that contact with blood represents an important cue for B. pertussis cells. 相似文献
Level set method [S. Osher, J. Sethian, J. Comput. Phys. 79 (1988) 12] is a highly robust and accurate computational technique for tracking moving interfaces in various application domains. It originates from the idea to view the moving front as a particular level set of a higher dimensional function, so the topological merging and breaking, sharp gradients and cusps can form naturally, and the effects of curvature can be easily incorporated. The resulting equations, describing interface surface evolution, are of Hamilton-Jacobi type and they are solved using techniques developed for hyperbolic equations. In this paper we describe an extension of the sparse field method for solving level set equations in the case of non-convex Hamiltonians, which are common in the simulations of the profile surface evolution during plasma etching and deposition processes. Sparse field method itself, developed by Whitaker [R. Whitaker, Internat. J. Comput. Vision 29 (3) (1998) 203] and broadly used in image processing community, is an alternative to the usual combination of narrow band and fast marching procedures for the computationally effective solving of level set equations. The developed procedure is applied to the simulations of 3D feature profile surface evolution during plasma etching process, that include the effects of ion enhanced chemical etching and physical sputtering, which are the primary causes of the Hamiltonian non-convexity. 相似文献
In this paper, we continue the research on formal treatment of attributes of information, based on the computational approach. In this scenario, the usefulness of advisory information is measured by the decrease in complexity of a problem we need to solve. We propose to model the time criticality via usefulness of a piece of information which is received during the computation. As a modeling tool, we use deterministic finite automata. 相似文献
Three series of hyperbranched urethane-acrylates (HB-UA), based on aliphatic hyperbranched polyesters and polyethyleneglycol acrylate, were prepared and evaluated for use in UV-curable coatings. UV-curing kinetics were monitored by FT-IR spectroscopy. The thermal and mechanical properties of UV-cured HB-UA were investigated by means of differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA) and correlated to the mechanical properties of coatings (hardness and flexibility). These new HB-UA's are very reactive and did not show oxygen inhibition. Obtained coatings have good mechanical properties and solvent resistance. 相似文献
The dependence of the critical concentration of the ternary system polymer-polymer-solvent on the molecular weight can be expressed by the relation where cc is the critical concentration, M? the appropriate average of the molecular weight of both polymers; A and cc∞ are constants for the given system. The possibility of determining the molecular weight of one of the polymers from the value cc and the molecular weight of the known polymer with the aid of equation (1) is discussed. 相似文献
We present improved variations of the BNDM algorithm for exact string matching. At each alignment our bit-parallel algorithms process a q-gram before testing the state variable. In addition we apply reading a 2-gram in one instruction. Our point of view is practical efficiency of algorithms. Our experiments show that the new variations are faster than earlier algorithms in many cases. 相似文献
Urban environments possess many regularities which can be efficiently exploited for 3D dense reconstruction from multiple
widely separated views. We present an approach utilizing properties of piecewise planarity and restricted number of plane
orientations to suppress reconstruction and matching ambiguities causing failures of standard dense stereo methods. We formulate
the problem of the 3D reconstruction in MRF framework built on an image pre-segmented into superpixels. Using this representation,
we propose novel photometric and superpixel boundary consistency terms explicitly derived from superpixels and show that they
overcome many difficulties of standard pixel-based formulations and handle favorably problematic scenarios containing many
repetitive structures and no or low textured regions. We demonstrate our approach on several wide-baseline scenes demonstrating
superior performance compared to previously proposed methods. 相似文献
Dynamic range of the scene can be significantly wider than the dynamic range of an image because of limitations of A/D conversion. In such a situation, numerous details of the scene cannot be adequately shown on the image. Standard industrial digital cameras are equipped with an auto-exposure function that automatically sets both the aperture value and cameras exposure time. When measuring a scene with atypical distribution of light and dark elements, the indicated auto-exposure time may not be optimal. The aim of work was to improve, with minimal cost, the performance of standard industrial digital cameras. We propose a low complexity method for creating HDR-like image using three images captured with different exposure times. The proposed method consists of three algorithms: (1) algorithm for estimating whether the auto-exposure time is optimal, (2) algorithm which determines exposure times for two additional images (one with shorter and another with longer than auto-exposure time), and (3) algorithm for HDR-like imaging based on fusion of three previously obtained images. Method is implemented on FPGA inserted into standard industrial digital camera. Results show that the proposed approach produces high quality HDR-like scene-mapped 8-bit images with minimal computational cost. All improvements may be noticed through the performance evaluation.