全文获取类型
收费全文 | 733篇 |
免费 | 65篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 7篇 |
化学工业 | 257篇 |
金属工艺 | 3篇 |
机械仪表 | 4篇 |
建筑科学 | 32篇 |
矿业工程 | 1篇 |
能源动力 | 14篇 |
轻工业 | 153篇 |
水利工程 | 15篇 |
石油天然气 | 8篇 |
无线电 | 43篇 |
一般工业技术 | 88篇 |
冶金工业 | 111篇 |
原子能技术 | 2篇 |
自动化技术 | 61篇 |
出版年
2023年 | 13篇 |
2022年 | 33篇 |
2021年 | 50篇 |
2020年 | 20篇 |
2019年 | 16篇 |
2018年 | 33篇 |
2017年 | 25篇 |
2016年 | 25篇 |
2015年 | 25篇 |
2014年 | 20篇 |
2013年 | 46篇 |
2012年 | 39篇 |
2011年 | 54篇 |
2010年 | 34篇 |
2009年 | 34篇 |
2008年 | 36篇 |
2007年 | 31篇 |
2006年 | 34篇 |
2005年 | 17篇 |
2004年 | 16篇 |
2003年 | 27篇 |
2002年 | 11篇 |
2001年 | 8篇 |
2000年 | 7篇 |
1999年 | 11篇 |
1998年 | 8篇 |
1997年 | 10篇 |
1996年 | 12篇 |
1995年 | 10篇 |
1994年 | 11篇 |
1993年 | 5篇 |
1992年 | 7篇 |
1990年 | 3篇 |
1989年 | 8篇 |
1988年 | 2篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1973年 | 2篇 |
1969年 | 2篇 |
1959年 | 2篇 |
1947年 | 2篇 |
1946年 | 2篇 |
排序方式: 共有799条查询结果,搜索用时 15 毫秒
1.
Cecilia Arriagada Viviana A. Cavieres Charlotte Luchsinger Alexis E. Gonzlez Vanessa C. Muoz Jorge Cancino Patricia V. Burgos Gonzalo A. Mardones 《International journal of molecular sciences》2020,21(22)
Protein trafficking is altered when normal cells acquire a tumor phenotype. A key subcellular compartment in regulating protein trafficking is the Golgi apparatus, but its role in carcinogenesis is still not well defined. Golgi phosphoprotein 3 (GOLPH3), a peripheral membrane protein mostly localized at the trans-Golgi network, is overexpressed in several tumor types including glioblastoma multiforme (GBM), the most lethal primary brain tumor. Moreover, GOLPH3 is currently considered an oncoprotein, however its precise function in GBM is not fully understood. Here, we analyzed in T98G cells of GBM, which express high levels of epidermal growth factor receptor (EGFR), the effect of stable RNAi-mediated knockdown of GOLPH3. We found that silencing GOLPH3 caused a significant reduction in the proliferation of T98G cells and an unexpected increase in total EGFR levels, even at the cell surface, which was however less prone to ligand-induced autophosphorylation. Furthermore, silencing GOLPH3 decreased EGFR sialylation and fucosylation, which correlated with delayed ligand-induced EGFR downregulation and its accumulation at endo-lysosomal compartments. Finally, we found that EGF failed at promoting EGFR ubiquitylation when the levels of GOLPH3 were reduced. Altogether, our results show that GOLPH3 in T98G cells regulates the endocytic trafficking and activation of EGFR likely by affecting its extent of glycosylation and ubiquitylation. 相似文献
2.
[Correction Notice: An erratum for this article was reported in Vol 31(2) of Canadian Psychology Psychologie Canadienne (see record 2007-08913-001). In the October 1989 issue (Vol. 30, No. 4, p. 697), Arnold Rincover's affiliation with the Ontario Institute for Studies in Education was incorrectly given as Associate Professor. He has been an Extramural Instructor at O.I.S.E.] Reviews the book, The parent-child connection by Arnold Rincover (1988). The parent-child connection is a well written book that offers valuable advice to help parents of young children evaluate and understand their children's behaviour. It also offers useful suggestions on managing child behaviour, although these parenting tips are most likely to be useful to those parents who least need them (i.e., those with numerous personal and social resources, whose children are presenting only minor behavioural difficulties). The two general themes of the book, child behaviour as communication and developmental norms as guidelines for deciding if behaviour is problematic, are well-suited to the purposes of a parent reference book. They offer an appropriate framework for discussing specific child behaviours and helping parents to determine if these behaviours are problematic in their children. This book is a welcome addition to the list of available parenting books, and may prove particularly useful for younger parents in need of accurate and understandable information about normal child development and behaviour. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
3.
Brown Charlotte; Abe-Kim Jennifer S.; Barrio Concepcion 《Canadian Metallurgical Quarterly》2003,34(1):10
What do health care practitioners need to know about providing adequate care for depressed minority women? This article examined the prevalence of depressive symptoms and clinical depression in ethnic minorities, the extent to which current health service utilization is congruent with needs, and the effectiveness of treatments provided to ethnic minorities in the primary care setting. The impact of ethnic minority women's sociocultural context on symptom expression and help-seeking behavior is also discussed. Finally, the clinical implications for accurate assessment and treatment of ethnic minority women by both medical and mental health practitioners working in the primary care sector are addressed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
4.
Three experiments investigated the suggestion that a predicted or primed stimulus commands less processing and consequently elicits a weaker CR than a stimulus that is not primed. In each experiment rats received initial training in which the presentation of each of 2 serial compounds, A-X and B-Y, was followed by the delivery of food. Subsequently, X's capacity to elicit the CR, approaching the site of food delivery, was assessed when X was preceded by Stimulus A (i.e., primed) or was presented after Stimulus B. Stimulus X elicited a more vigorous response when it was presented after B than when it followed A. These results show that the ability of one event to elicit its CR is reduced if its presentation has been predicted by some other event. This negative priming effect supports one aspect of A. R. Wagner's (1981) model of Pavlovian conditioning. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
5.
Finding new applications for ceramic materials requires a better knowledge of thermal fatigue behaviour. However, result-scattering inherent to thermal fatigue and duration of a thermal fatigue cycle lead to a lack of experimental results. For these reasons, we have developed a new approach that permits the determination of a relevant stress intensity factor exponent n with a minimum testing sample number. From knowledge of the distribution function of artificial cracks, the analytical formula of the failure probability F(N) can be completely determined. Thus, it is possible to calculate n from a correlation of F(N) with experimental results obtained for only one temperature difference. Correlations between theoretical curves F(N) and experimental results, conducted for two temperature differences, lead to the same value of n. This and the good agreement between the experimental points and the theoretical curves validate this new approach. 相似文献
6.
Thermal shock and thermal fatigue study of ceramic materials on a newly developed ascending thermal shock test equipment 总被引:1,自引:0,他引:1
P. K. Panda T. S. Kannan J. Dubois C. Olagnon G. Fantozzi 《Science and Technology of Advanced Materials》2002,3(4):44
A test equipment was designed to study thermal shock and thermal fatigue of ceramic materials subjected to fast heating (ascending). The equipment was designed to generate thermal stress in a test specimen by heating one surface of it by an oxy-hydrogen flame while cooling the opposite surface. The sample cracked when thermal stress exceeded its mechanical strength. The in situ crack formation was detected by an acoustic emission system coupled to the set up. The hot zone temperature was measured by an infra red pyrometer. The equipment was also designed to run thermal fatigue test cycles in automatic mode between two selected temperatures. The temperature and thermal stress distribution in the test specimen were modelled using finite element software. The effect of temperature distribution of the top and bottom surfaces on thermal stresses was studied. It was observed that the thermal stress is very sensitive to the temperature distribution on the top surface and maximum near the periphery of the top surface. This was in agreement with the experimental results in which the cracks were originated from the periphery of top surface. It was also observed that the failure temperature was higher for thicker samples. 相似文献
7.
8.
9.
10.
Charlotte Bussienne Roland Marquet Jean-Christophe Paillart Serena Bernacchi 《International journal of molecular sciences》2021,22(6)
Protein post-translational modifications (PTMs) play key roles in eukaryotes since they finely regulate numerous mechanisms used to diversify the protein functions and to modulate their signaling networks. Besides, these chemical modifications also take part in the viral hijacking of the host, and also contribute to the cellular response to viral infections. All domains of the human immunodeficiency virus type 1 (HIV-1) Gag precursor of 55-kDa (Pr55Gag), which is the central actor for viral RNA specific recruitment and genome packaging, are post-translationally modified. In this review, we summarize the current knowledge about HIV-1 Pr55Gag PTMs such as myristoylation, phosphorylation, ubiquitination, sumoylation, methylation, and ISGylation in order to figure out how these modifications affect the precursor functions and viral replication. Indeed, in HIV-1, PTMs regulate the precursor trafficking between cell compartments and its anchoring at the plasma membrane, where viral assembly occurs. Interestingly, PTMs also allow Pr55Gag to hijack the cell machinery to achieve viral budding as they drive recognition between viral proteins or cellular components such as the ESCRT machinery. Finally, we will describe and compare PTMs of several other retroviral Gag proteins to give a global overview of their role in the retroviral life cycle. 相似文献