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排序方式: 共有56条查询结果,搜索用时 453 毫秒
1.
Rui Morimoto Chisato Yokomori Akiko Kikkawa Akira Izumi Hideki Matsumura 《Thin solid films》2003,430(1-2):230-235
In this paper, bulk-Si metal–oxide–semiconductor field effect transistors (MOSFETs) are fabricated using the catalytic chemical vapor deposition (Cat-CVD) method as an alternative technology to the conventional high-temperature thermal chemical vapor deposition. Particularly, formation of low-resistivity phosphorus (P)-doped poly-Si films is attempted by using Cat-CVD-deposited amorphous silicon (a-Si) films and successive rapid thermal annealing (RTA) of them. Even after RTA processes, neither peeling nor bubbling are observed, since hydrogen contents in Cat-CVD a-Si films can be as low as 1.1%. Both the crystallization and low resistivity of 0.004 Ω·cm are realized by RTA at 1000 °C for only 5 s. It is also revealed that Cat-CVD SiNx films prepared at 250 °C show excellent oxidation resistance, when the thickness of films is larger than approximately 10 nm for wet O2 oxidation at 1100 °C. It is found that the thickness required to stop oxygen penetration is equivalent to that for thermal CVD SiNx prepared at 750 °C. Finally, complementary MOSFETs (CMOSs) of single-crystalline Si were fabricated by using Cat-CVD poly-Si for gate electrodes and SiNx films for masks of local oxidation of silicon (LOCOS). At 3.3 V operation, less than 1.0 pA μm−1 of OFF leakage current and ON/OFF ratio of 107–108 are realized, i.e. the devices can operate similarly to conventional thermal CVD process. 相似文献
2.
Identification of SYS1 as a Host Factor Required for Shiga Toxin-Mediated Cytotoxicity in Vero Cells
Chisato Sakuma Tsuyoshi Sekizuka Makoto Kuroda Kentaro Hanada Toshiyuki Yamaji 《International journal of molecular sciences》2021,22(9)
Shiga toxin (STx) or Vero toxin is a virulence factor produced by enterohemorrhagic Escherichia coli. The toxin binds to the glycosphingolipid globotriaosylceramide (Gb3) for its entry, and causes cell death by inhibiting ribosome function. Previously, we performed a loss-of-function screen in HeLa cells using a human CRISPR knockout (KO) library and identified various host genes required for STx-induced cell death. To determine whether this library targeted to the human genome is applicable to non-human primate cells and to identify previously unrecognized factors crucial for STx-induced cell death, we herein performed a similar screen in the African green monkey kidney-derived Vero C1008 subline. Many genes relevant to metabolic enzymes and membrane trafficking were enriched, although the number of enriched genes was less than that obtained in the screening for HeLa cells. Of note, several genes that had not been enriched in the previous screening were enriched: one of these genes was SYS1, which encodes a multi-spanning membrane protein in the Golgi apparatus. In SYS1 KO Vero cells, expression of Gb3 and sphingomyelin was decreased, while that of glucosylceramide and lactosylceramide was increased. In addition, loss of SYS1 inhibited the biosynthesis of protein glycans, deformed the Golgi apparatus, and perturbed the localization of trans-Golgi network protein (TGN) 46. These results indicate that the human CRISPR KO library is applicable to Vero cell lines, and SYS1 has a widespread effect on glycan biosynthesis via regulation of intra-Golgi and endosome–TGN retrograde transports. 相似文献
3.
Chisato Takahashi Deepak K. Pattanayak Takashi Shirai Masayoshi Fuji 《Journal of the European Ceramic Society》2013,33(4):629-635
The purpose of this study is to develop a simple method to observe the surface morphology of non-conductive material in its hydrated condition. Here porous hydroxyapatite (HAp) green body prepared by gelcasting process was considered as a case study, and, the resultant body was subsequently treated with hydrophilic ionic liquid (IL). The surface morphology and the pore structure of the IL-treated porous HAp green body were successfully observed even in hydrated condition without any charging using field emission scanning electron microscope (FE-SEM). Results showed that the pore diameter from 300 to 600 μm in as-prepared green body was reduced to 100–300 μm in the sample sintered at 1000 °C. Raman results showed that the IL forms weak hydrogen bond with water molecules within the sample and, that prevents from drying in vacuum condition. In addition, the IL acts as a conducting media for HAp ceramics to be observed under FE-SEM. 相似文献
4.
Kazuaki Yanagisawa Keishiro Ito Chisato Katsuki Kei Kawashima Masashi Shirabe 《Scientometrics》2010,84(3):563-573
An outcome of nuclear safety research (NSR) done by JAERI (Japan Atomic Energy Research Institute) was case studied by the
bibliometric method. (1) For LOCA (loss-of-coolant accident) a domestic share of JAERI in monoclinic research paper was 63%
at the past (20) 1978–1982 but was decreased to 40% at the present 1998–2002. For co-authored papers a domestic share between
JAERI and PS (public sectors) was zero at past (20) but increased to 4% at the present. Research cooperation is active between
Tokyo University and JAERI or between JAERI and Nagoya University. (2) It is revealed that LOCA outputs born by NSR-JAERI
reflected partly to those of the Safety Licensing Guidelines, however, a share of NSR-JAERI could not determined due to the
lack of necessary information in the Guideline. 相似文献
5.
Yusuke Suzuki Chisato Takenaka Rie Tomioka Hiromi Tsubota Yuka Takasaki Tomonari Umemura 《Mine Water and the Environment》2016,35(3):265-272
Bryophytes with high As accumulation affinity were identified in the aquatic environment. We surveyed a stream near copper mine tailings and then conducted laboratory experiments to confirm As accumulation in the bryophytes with high As affinity. We found that a moss, Scopelophila cataractae, accumulates As in addition to Cu in aquatic environments and confirmed it in laboratory experiments. The highest value for As in S. cataractae from the field survey was 1300 mg/kg dry weight at relatively low As concentrations in the stream water (0.005 mg/L). In addition, Brachythecium plumosum and Rhynchostegium riparioides may also be useful bryophytes for accumulation of Cu and As, though the mechanisms of As accumulation might differ between these two bryophytes and S. cataractae. 相似文献
6.
A new design approach for a test chip developed to shorten the debugging cycle time in fabrication is described. This approach meets the requirements for failure analysis as well as parametric and statistical analyses. Particular attention is devoted to accurate defect density estimation and to locating individual defects. This is done by designing test structures suitable for both electrical measurements and failure analysis. A specially designed test chip, named YTEG, is used to evaluate 0.5-μm CMOS process technologies, and confirms the effectiveness of the chip. 相似文献
7.
The immunotoxic effects of some anthropogenic pollutants on aquatic organisms are among the causes of concern over the presence of these pollutants in the marine environment. The immune system is part of an organism’s biological defense necessarily for homeostasis. Thus, the immunotoxicological impacts on aquatic organisms are important to understand the effects of pollutant chemicals in the aquatic ecosystem. When aquatic organisms are exposed to pollutant chemicals with immunotoxicity, it results in poor health. In addition, aquatic organisms are exposed to pathogenic bacteria, viruses, parasites, and fungi. Exposure to pollutant chemicals has reportedly caused aquatic organisms to show various immunotoxic symptoms such as histological changes of lymphoid tissue, changes of immune functionality and the distribution of immune cells, and changes in the resistance of organisms to infection by pathogens. Alterations of immune systems by contaminants can therefore lead to the deaths of individual organisms, increase the general risk of infections by pathogens, and probably decrease the populations of some species. This review introduced the immunotoxicological impact of pollutant chemicals in aquatic organisms, including invertebrates, fish, amphibians, and marine mammals; described typical biomarkers used in aquatic immunotoxicological studies; and then, discussed the current issues on ecological risk assessment and how to address ecological risk assessment through immunotoxicology. Moreover, the usefulness of the population growth rate to estimate the immunotoxicological impact of pollution chemicals was proposed. 相似文献
8.
Kaidi Nie Felix von Drigalski Joshua C. Triyonoputro Chisato Nakashima Yoshiya Shibata Yoshinori Konishi 《Advanced Robotics》2020,34(7-8):477-498
ABSTRACTIn this paper, we describe the approach of Team O2AS to complete the task-board task of the World Robot Challenge 2018, held in Tokyo. We use a custom gripper and graspable tools with in-built compliance to work with various kinds of parts, increase robustness against uncertainties, and to avoid complicated control strategies. The robots are able to finish all the sub-tasks without the need to exchange grippers. The main idea is to use mechanical compliance and self-centering mechanisms to deal with uncertainty. This is achieved by aligning the objects using either the gripper and tools, or by the design of the robot motions. 相似文献
9.
Felix von Drigalski Chisato Nakashima Yoshiya Shibata Yoshinori Konishi Joshua C. Triyonoputro Kaidi Nie 《Advanced Robotics》2020,34(7-8):514-530
ABSTRACTIn this article, we propose a versatile robotic system for kitting and assembly tasks which uses no jigs or commercial tool changers. Instead of specialized end effectors, it uses its two-finger grippers to grasp and hold tools to perform subtasks such as screwing and suctioning. A third gripper is used as a precision picking and centering tool, and uses in-built passive compliance to compensate for small position errors and uncertainty. A novel grasp point detection for bin picking is described for the kitting task, using a single depth map. Using the proposed system we competed in the Assembly Challenge of the Industrial Robotics Category of the World Robot Challenge at the World Robot Summit 2018, obtaining 4th place and the SICE award for lean design and versatile tool use. We show the effectiveness of our approach through experiments performed during the competition. 相似文献
10.
This paper describes the inhibitory activities of cholesterol derivatives such as cholesterol, sodium cholesteryl sulfate,
cholesteryl-5α, 6α-epoxide, cholesteryl chloride, cholesteryl bromide, and cholesteryl hemisuccinate (compounds 1–6, respectively) against DNA polymerase (pol), DNA topoisomerase (topo), and human cancer cell growth. Among the compounds
tested, compounds 2 and 6 revealed themselves to be potent inhibitors of animal pols, and the IC50 values for pols were 0.84–11.6 and 2.9–148 μM, respectively. Compounds 2, 3 and 6 inhibited the activity of human topo II, with IC50 values of 5.0, 12.5 and 120 μM, respectively. Compounds 2, 3 and 6 also suppressed human cancer cell (promyelocytic leukemia cell line, HL-60) growth, and LD50 values were 8.8, 20.2 and 72.3 μM, respectively, suggesting that cell growth inhibition had the same tendency as the inhibition
of topos rather than pols. Compounds 2 and 6 arrested the cells in S and G2/M phases, compound 3 arrested the cells in the G2/M phase, and these compounds also increased sub-G1 phase in the cell cycle. These results suggested
that the effect of cell cycle arrest might be effective on both pols and topos activities. From these findings, the action
mode of cholesterol derivatives as anti-cancer compounds is discussed. 相似文献