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排序方式: 共有144条查询结果,搜索用时 46 毫秒
1.
F Ildan S Polat AI G?cer A Oner T Isbir UO Mete M Kaya A Karadayi 《Canadian Metallurgical Quarterly》1996,138(3):338-345
The sodium-potassium activated and magnesium dependent adenosine-5'-triphosphatase (Na(+)-K(+)/Mg(+2) ATPase EC.3.6.1.3.) activity and lipid peroxidation and early ultrastructural findings were determined in rat brain at the acute stage of ischaemia produced by permanent unilateral occlusion of the middle cerebral artery (MCA). The effects of the pretreatment with intravenous high-dose methylprednisolone (MP) on these biochemical indices and ultrastructural findings were also evaluated in the same model. The rats were divided into four groups. In group I, 10 rats were used to determine Na(+)-K(+)/Mg(+2) ATPase activity and the extent of lipid peroxidation by measuring the malondialdehyde (MDA) content and normal ultrastructural findings. In group II on 20 rats, only subtemporal craniectomy was done in order to determine the effects of the surgical procedure on these indices and findings. This group was treated intravenously with saline solution before occlusion. In group III with MCA occlusion, saline solution was administered intravenously to 20 rats in the same amount of methylprednisolone used in group IV, ten minutes before the occlusion. In Group IV, a single high-dose (30 mg/kg) of methylprednisolone was administered intravenously, ten minutes before occlusion in 20 rats. After occlusion of the middle cerebral artery, Na(+)-K(+)/Mg(+2) ATPase activity was decreased promptly in the first ten minutes in the ischaemic hemisphere and remained at a lower level than the contralateral hemispheres in the same group and the normal levels in group I, during 120 minutes of ischaemia. A single dose methylprednisolone pretreatment prohibited the inactivation of Na(+)-K(+)/Mg(+2) ATPase. On the other hand, there was significant difference in malondialdehyde content between group I and group III. Malondialdehyde levels were significantly increased following ischaemia and a non-significant increase was observed in the contralateral hemisphere. Methylprednisolone treatment significantly decreased malondialdehyde content on the side of the ischaemic hemisphere. We conclude that there is a positive relationship between membrane-bound enzyme Na(+)-K(+)/Mg(+2) ATPase activity, malondialdehyde content and early ultrastructural changes in the treated group with MP. These data suggest that the pretreatment injection of high doses (30 mg/kg) methylprednisolone contribute to the protection of the brain from ischaemia with stabilization of the cell membrane by effecting the lipid peroxidation and the activation of Na(+)-K(+)/Mg(+2) ATPase. 相似文献
2.
J. J. Quist W. J. A. Dhert W. Visser F. C. Oner A. J. Verbout B. P. Meij H. A. W. Hazewinkel 《Journal of materials science. Materials in medicine》1995,6(12):731-738
In the present study the potential of Polyactive® bilayer sheets in the prevention of scar tissue formation after spinal surgery was investigated. Eight adult beagle dogs underwent 3 laminectomies at three levels (L2, L4 and L6). According to a randomized implantation schedule a Polyactive® sheet or autogenous fat graft was placed in the defect. The third site served as a control without implant. After 4 or 12 weeks (4 dogs per period) the dogs were sacrificed and histological sections were prepared. The Polyactive® treated defects showed partial closure by newly formed bone. The Polyactive® was encapsuled by a thin fibrous tissue layer. Ventral to the defect, dense fibrous tissue was present which was separated from the dura by the Polyactive® sheet in all cases. In some cases fibrous tissue was present between the implant capsule and the dura. In the fat graft group there were no signs of closure of the defect but most sites showed fibrous tissue at the edges of the graft, which was in 4 sites continous with the dura mater. Fibrosis and degeneration of the fat grafts were seen. All control defects showed partial closure by newly formed bone, and ventral to the defect extensive fibrous tissue, which was in 50% continous with the dura mater. Other sections showed loose connective tissue in contact with the dura mater. It is concluded that Polyactive® has a potential as a mechanical barrier in the prevention of adhesions between the dorsal spinal muscles and the dura mater. 相似文献
3.
The influence of grinding technique on the liberation of clinker minerals and cement properties 总被引:1,自引:0,他引:1
This paper describes a study of the relationship between the physical, chemical and mineralogical parameters of cement products obtained by different grinding mechanisms namely high pressure grinding rolls (HPGR) and ball milling, and their effects upon the properties of cements prepared from the ground clinker. Samples were prepared as narrow size fractions and also as distribution samples. Characterization parameters were ascertained by using XRF, laser sizing, Blaine and BET surface area and image analysis methods. HPGR grinding resulted in higher degrees of liberation of clinker phases arising from the intergranular breakage along the grain boundaries compared to ball mill grinding. As for service properties, water demand of HPGR products was higher than ball mill products resulting from high micro fissured structure. Despite high liberation of particularly alite mineral in HPGR grinding, the compressive strength of ball mill products was slightly higher than HPGR products for narrow size samples. Finally, particle size distribution effect on strength was more obvious for distribution samples; generally ball milling gave higher strength values. 相似文献
4.
B. O. Solomon M. D. Oner L. E. Erickson S. S. Yang 《American Institute of Chemical Engineers》1984,30(5):747-757
Pirt's model for microbial growth and product formation are reparameterized to obtain multiresponse models with common parameters. The dependent variables in the models are related through the available electron and carbon balance constraints. Covariance adjustment is used to reduce the growth model to a unit variate linear model with covariates. Therefore, standard multiple regression programs can be used to obtain combined point and interval estimates of true biomass energetic yield, true product yield and maintenance coefficient. This approach may yield “better” estimates than the maximum likelihood approach when an appropriately selected subset of covariates is used. Nonlinear estimation procedures are also considered; these procedures are efficient with few responses; however, as the number of responses per observation increase, they may require a lot of computing time. For illustration several data from the biochemical engineering literature are analyzed by the proposed methods. 相似文献
5.
Didem Denizer Ibrahim M. A. ElSherbiny Mathias Ulbricht Stefan Panglisch 《化学,工程师,技术》2021,93(9):1401-1407
Surface patterning is a recent promising approach to promote performance of pressure-driven membranes in water treatment and desalination. Nevertheless, knowledge about foulant deposition mechanisms, especially at early stage of filtration, is still lacking. The applicability of particle imaging velocimetry to study fluid characteristics atop surface patterned thin-film composite membranes was investigated at different operating conditions. This work is an important first step toward reliable understanding of the impacts of topographical membrane surface modification on hydrodynamic conditions and foulant deposition mechanisms. 相似文献
6.
Computational Economics - In Economic Development, human capital was previously seen as production factors but gradually evolved into endogenous growth theories. Most of the previous studies have... 相似文献
7.
The combined application of electron microscopy (EM) is frequently used for the microstructural investigation of biological specimens and plays two important roles in the quantification and in gaining an improved understanding of biological phenomena by making use of the highest resolution capability provided by EM. The possibility of imaging wet specimens in their "native" states in the environmental scanning electron microscope (ESEM) at high resolution and large depth of focus in real time is discussed in this paper. It is demonstrated here that new features can be discovered by the elimination of even the least hazardous approaches in some preparation techniques, that destroy the samples. Since the analysis conditions may influence the morphology and the extreme surface sensitivity of living biological systems, the results obtained from the same cultured cell with two different ESEM modes (Lvac mode and wet mode) were compared. This offers new opportunities compared with ESEM-wet/Lvac-mode imaging, since wet-mode imaging involves a real contrast and gives an indication of the changes in cell morphology and structure required for cell viability. In this study, wet-mode imaging was optimized using the unique ability of cell quantities for microcharacterization in situ giving very fine features of topological effects. Accordingly, the progress is reported by comparing the results of these two modes, which demonstrate interesting application details. In general, the functional comparisons have revealed that the fresh unprocessed Saccharomyces cerevisiae cells (ESEM-wet mode) were essentially unaltered with improved and minimal specimen preparation timescales, and the optimal cell viability degree was visualized and also measured quantitatively while the cell size remained unchanged with continuous images. 相似文献
8.
Didem Ag Seleci Viktor Maurer Firat Baris Barlas Julian Cedric Porsiel Bilal Temel Elcin Ceylan Suna Timur Frank Stahl Thomas Scheper Georg Garnweitner 《International journal of molecular sciences》2021,22(9)
The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEGNIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma. 相似文献
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