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排序方式: 共有69条查询结果,搜索用时 15 毫秒
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Kyohei Terao Chihiro Masuda Ryo Inukai Murat Gel Hidehiro Oana Masao Washizu Takaaki Suzuki Hidekuni Takao Fusao Shimokawa Fumikazu Oohira 《IET nanobiotechnology / IET》2016,10(3):124
Optical tweezers are powerful tools for manipulating single DNA molecules using fluorescence microscopy, particularly in nanotechnology‐based DNA analysis. We previously proposed a manipulation technique using microstructures driven by optical tweezers that allows the handling of single giant DNA molecules of millimetre length that cannot be manipulated by conventional techniques. To further develop this technique, the authors characterised the microstructures quantitatively from the view point of fabrication and efficiency of DNA manipulation under a fluorescence microscope. The success rate and precision of the fabrications were evaluated. The results indicate that the microstructures are obtained in an aqueous solution with a precision ∼50 nm at concentrations in the order of 106 particles/ml. The visibility of these microstructures under a fluorescence microscope was also characterised, along with the elucidation of the fabrication parameters needed to fine tune visibility. Manipulating yeast chromosomal DNA molecules with the microstructures illustrated the relationship between the efficiency of manipulation and the geometrical shape of the microstructure. This report provides the guidelines for designing microstructures used in single DNA molecule analysis based on on‐site DNA manipulation, and is expected to broaden the applications of this technique in the future.Inspec keywords: DNA, molecular biophysics, fluorescence, optical microscopy, radiation pressure, biological techniquesOther keywords: optically driven microstructures, single DNA molecule analysis, fluorescence microscopy, optical tweezers, nanotechnology‐based DNA analysis, manipulation technique, aqueous solution, fine tune visibility, yeast chromosomal DNA molecules, geometrical shape, on‐site DNA manipulation 相似文献
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This paper is an overview of the high-speed DRAM architecture developments. We discuss developments on density growth, interface technology, memory-core architecture, and DRAM+ASIC technology. We can find the developments of density as 2× growth instead of 4× by each generation. Interface technologies will have a tendency to use the terminated bus structure for higher data rate. Memory-core architecture developments are the trials for actual bandwidth improvements. DRAM+ASIC technologies seem to require universal interface solutions. We tried to show that no single solution is able to cover the wide diversity of future system requirements 相似文献
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Poly(ε-caprolactone) (PCL) is one of the ecofriendly biodegradable polymers with excellent moldability but with rather low mechanical properties especially for the industrial and biomedical use. In this research, to overcome the problem, the two types of cellulose nanofibers, the cellulose acetate nanofibers (CA-NF) and the cellulose nanofibers (C-NF), were composited into PCL for the enhancement of the mechanical properties of PCL. CA-NF were prepared by electrospinning and converted into C-NF afterward by deacetylation. It was found that the Young's modulus of the CA-NF/PCL composite at the fiber concentration of 35 wt% significantly increased by ~3 times as compared with that of neat PCL, whereas C-NF/PCL of the same fiber concentration also increased by ~4.5 times. It was also found that the Young's moduli of CA-NF/PCL nearly reached the theoretical values calculated by the equation suggested by Tsai, but that the Young's moduli of C-NF/PCL could not reach the theoretical values. It indicates that CA-NF possessed better compatibility with PCL than C-NF, agreeing well with the fracture-surface analyses of the two composites by the scanning electron microscopy. 相似文献
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A finite-difference time–space numerical algorithm for the analysis of transient electromagnetic fields is proposed based on the expression of the field vectors in terms of vector potential functions. The method allows for both integral and finite-difference calculation of the potential functions (PF). Both approaches can be applied in combination. Since it is based on the time-domain integral-equation approach, it reduces the 3D problem to a 2D one, and only the tangential vector potential components are involved in the calculation. The consistency of the method has been proved by simulations of Gaussian pulse propagation in a homogeneous and an open-end microstripline and a thin-plate scattering problem. © 1998 John Wiley & Sons, Inc. Int J RF and Microwave CAE 8: 56–67, 1998. 相似文献
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Kenji TakadaYuta Ishigami Junji Inukai Yuzo NagumoHiroshi Takano Hiroyuki Nishide 《Journal of power sources》2011,196(5):2635-2639
Visualization inside polymer electrolyte fuel cells (PEFCs) is important for elucidating reaction distributions to improve the performance and durability of the cells. An O2-sensitive porphyrin luminescent dye film was used to visualize oxygen partial pressures and water blockages simultaneously in triple-serpentine gas flow channels in an operating PEFC. Water droplets formed near the exit of a gas-flow channel lowered the oxygen partial pressure noticeably over the channel by blocking air flow near the entrance. Meanwhile, air was continuously supplied from the other channels through the gas diffusion layer, thus allowing power to be generated in the blocked channel. With water blockages, however, the catalyst layer under the channel became flooded by the water produced during the reaction, and the flooded state continued to exist in the catalyst and/or porous layers, even after blowing the water droplet out, so that the power generation was lowered along the channel. 相似文献
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T Inukai Y Fujiwara Y Aso A Yamamoto Y Takemura 《Canadian Metallurgical Quarterly》1998,29(3-4):185-198
A 52-year-old Japanese male complained of palpitations and excessive sweating. He showed evidence of hyperthyroidism, but without suppression of the serum thyroid-stimulating hormone (TSH). On admission, the serum level of the alpha-subunit of TSH was elevated, but tests for thyroid autoantibodies were negative. The TSH response to thyrotropin-releasing hormone (TRH) was blunted. Imaging studies revealed a pituitary tumor 2 cm in diameter. Administration of methimazole lowered the serum levels of thyroid hormones, and elevated the serum level of TSH. These findings led to the diagnosis of a thyrotropin-secreting pituitary tumor. Immunohistochemical analysis and electron microscopic findings verified the diagnosis. 相似文献