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Verhelst SH Michiels PJ van der Marel GA van Boeckel CA van Boom JH 《Chembiochem : a European journal of chemical biology》2004,5(7):937-942
Aminoglycoside antibiotics, which are able to selectively bind to RNA, are considered to be an important lead in RNA-targeting drug discovery. In this study, surface plasmon resonance (SPR) was employed to explore the interaction of aminoglycosides with known tobramycin-binding RNA hairpins (aptamers) and an unrelated RNA hairpin. It was established that aminoglycosides have multiple interactions with RNA hairpins. Unexpectedly, the different hairpins showed comparable affinity for a set of related aminoglycosides. The observed absence of selectivity presents an extra hurdle in the discovery of novel aminoglycosides as specific drugs that target defined RNA hairpins. 相似文献
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OBJECTIVE: Effects of four types of automation support and two levels of automation reliability were examined. The objective was to examine the differential impact of information and decision automation and to investigate the costs of automation unreliability. BACKGROUND: Research has shown that imperfect automation can lead to differential effects of stages and levels of automation on human performance. Method: Eighteen participants performed a "sensor to shooter" targeting simulation of command and control. Dependent variables included accuracy and response time of target engagement decisions, secondary task performance, and subjective ratings of mental work-load, trust, and self-confidence. RESULTS: Compared with manual performance, reliable automation significantly reduced decision times. Unreliable automation led to greater cost in decision-making accuracy under the higher automation reliability condition for three different forms of decision automation relative to information automation. At low automation reliability, however, there was a cost in performance for both information and decision automation. CONCLUSION: The results are consistent with a model of human-automation interaction that requires evaluation of the different stages of information processing to which automation support can be applied. APPLICATION: If fully reliable decision automation cannot be guaranteed, designers should provide users with information automation support or other tools that allow for inspection and analysis of raw data. 相似文献
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In this paper a numerical solution is proposed for designing telescopic manipulators when workspace is prescribed through few suitable points. An algorithm is outlined by using an algebraic formulation for the workspace boundary and numerical solution is worked out by using a Newton–Raphson technique to solve a proper design problem. Numerical examples are reported to discuss computational efforts and solution characteristics. 相似文献
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Davidson Andrew R.; Yantis Steven; Norwood Marel; Montano Daniel E. 《Canadian Metallurgical Quarterly》1985,49(5):1184
The synthesis of 2 separate lines of inquiry—research on information integration and longitudinal studies of attitudes—prompted the hypothesis that the degree of consistency between attitudes and behavior will increase as a function of the amount of information available about the attitude object. This hypothesis was tested in 3 longitudinal studies, ranging in length from 4 days to 4 mo, that investigated the following behaviors: voting for candidates for political office, voting for 2 social-policy election initiatives, and having an influenza vaccination. Ss were 62 undergraduates (Study 1), 270 undergraduates (Study 2), and 299 27–92 yr old veterans (Study 3). In support of the hypothesis, amount of information moderated the consistency between attitudes and behavior in each study, and the significance of this relation remained even after controlling for the effects of other potential moderators, including prior direct behavioral experience with the attitude object and attitude certainty. Consistent with previous research, direct behavioral experience was also a determinant of attitude–behavior consistency, and this relation was independent of the effect of amount of information for the behavior of having an influenza vaccination. Discussion focuses on the interrelation among moderators of attitude–behavior consistency and on the theoretical implications of the findings. (52 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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A study has been made of induction bridges used in low temperature physics.In Part 1 the design of a mutual inductance bridge of the Hartshorn type is discussed. This design is based on a critical analysis of impurity effects of the different parts of the Hartshorn bridge. With this equipment frequencies up to 0.5 MHz can be used. Two methods have been developed to examine the secondary signal. In one of these use has been made of AD conversion techniques. In the other one, the secondary signal, produced by a superconducting sample, which is generally distorted, is analysed by using a Fourier expansion.In Part 2 equipment is described which enables us to measure the phase and amplitude of the harmonics of the output signal of the bridge. For synchronous detection a reference signal of the same frequency of the harmonic of interest is required. This reference signal is generated from the input signal of the bridge by means of a digital frequency multiplier with programmable multiplication factor N.In Part 3 some experimental results, showing the possibilities of the equipment, on some superconductors are presented. 相似文献