In Vitro Characterization of Human CD24hiCD38hi Regulatory B Cells Shows CD9 Is Not a Stable Breg Cell Marker

Regulatory B (Breg) cells are endowed with immune suppressive functions. Various human and murine Breg subtypes have been reported. While interleukin (IL)-10 intracellular staining remains the most reliable way to identify Breg cells, this technique hinders further essential functional studies. Recent findings suggest that CD9 is an effective surface marker of murine IL-10 competent Breg cells. However, the stability of CD9 and its relevance as a unique marker for human Breg cells, which have been widely characterized as CD24^hiCD38^hi, have not been investigated. Here, we demonstrate that CD9 expression is sensitive to in vitro B cell stimulations. CD9 expression could either be re-expressed or downregulated in purified CD9-negative B cells and CD9-positive B cells, respectively. We found no significant differences in the Breg differentiation capacity of the CD9-negative and CD9-positive B cells. Furthermore, CD9-positive B cells co-express CD40 and CD86, suggesting their nature as B cell activation or co-stimulatory molecules, rather than regulatory ones. Therefore, we report the relatively unstable CD9 as a distinct surface molecule, indicating the need for further research for a more reliable marker to purify human Breg cells.     

Influence of surface energy on the nanoindentation response of elastically-layered viscoelastic materials

In the context of integrated nonlinear viscoelastic contact mechanics, a nonlinear finite element model is developed to predict and analyze the quasistatic response of nanoindentation problems of an elastically-layered viscoelastic materials considering the surface elasticity effects. Effects of surface energy are accounted for by employing the Gurtin–Murdoch continuum model for surface elasticity. The linear viscoelastic response is modeled by the Schapery’s creep model with a Prony’s series to express the transient component in the creep compliance. The viscoelastic constitutive equations are cast into a recursive form that needs only the previous time increment rather than the entire strain history. To satisfy the contact constraints exactly, the Lagrange multiplier method is adopted to enforce the contact conditions into the system. The equilibrium indentation configuration is obtained through the Newton–Raphson iterative procedure. The developed model is verified then applied to investigate the quasistatic nanoindentation response of two different indentation problems with different geometry and loading conditions. Results show the significant effects of surface energy and viscoelasticity on the quasistatic nanoindentation response.     

Characterization and Properties of Recycled Polypropylene/Coconut Shell Powder Composites: Effect of Sodium Dodecyl Sulfate Modification

This research work focuses on the utilization of coconut shell powder (CSP) as filler in recycled polypropylene (rPP). Sodium Dedecyl Sulfate (SDS) was used as coupling agent in these composites. The effect of filler content and SDS on tensile properties, thermal properties, water absorption and morphology of rPP/CSP composites were investigated. In this study, modified rPP/CSP composites with SDS show significant increased tensile propertied, thermal stability, crystallinity and low water absorption compared unmodified rPP/CSP composites. Those improvements were contributed by the coupling effect of SDS.     

A technique for improving gain and noise figure of common-gate wideband LNAs

This paper presents a novel compensation design for regulators, i.e., modified NMCF (nested Miller compensation with feedforward Gm stage), resulting in a linear LDO (low dropout) regulator whose performance is independent of the off-chip capacitor and its ESR (equivalent series resistor). The proposed compensation method ensures the stability of the feedback loop and the sufficient phase margin of the LDO regulator. Besides, the transient response become faster. The analysis of the stability is derived to solidify the proposed design. The proposed design is implemented using TSMC 0.35 μm 2P4M CMOS process. The results verify the performance and the stability on silicon. The power supply rejection ratio is 25 dB @ [200 Hz, 3 MHz], [50 Ω, 500 Ω] provided that the input voltage varies from 4 to 5 V.     

Polyacrylonitrile–poly(1-vinyl pyrrolidone-co-vinyl acetate) blend based gel polymer electrolytes incorporated with sodium iodide salt for dye-sensitized solar cell applications

New gel polymer electrolytes (GPEs) were prepared via the blending of a polyacrylonitrile polymer and a poly(1-vinyl pyrrolidone-co-vinyl acetate) copolymer. The sodium iodide (NaI) salt concentration was varied for each GPE sample. From ionic conductivity (σ) studies, we observed that the sample with a 40 wt % NaI salt content (N40) showed the highest σ of 3.54 × 10⁻³ ± 0.05 S/cm at room temperature, and all of the GPE samples obeyed Arrhenius behavior. The dielectric properties of the GPE samples were also analyzed to study the electrical polarization of the materials. The developed GPE samples were also characterized with X-ray diffraction and Fourier transform infrared spectroscopy. We also then used the developed GPE samples for the fabrication of dye-sensitized solar cells by sandwiching them between a photoanode and Pt counter electrode for photovoltaic studies. The highest photovoltaic performance was achieved by N40, with an efficiency of 3.04%. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47810.     

Mechanical characteristics of oil palm fiber reinforced thermoplastics as filament for fused deposition modeling (FDM)

Fibers are increasingly in demand for a wide range of polymer composite materials. This study's purpose was the development of oil palm fiber (OPF) mixed with the thermoplastic material acrylonitrile butadiene styrene (ABS) as a composite filament for fused deposition modeling (FDM). The mechanical properties of this composite filament were then analyzed. OPF is a fiber extracted from empty fruit bunches, which has proved to be an excellent raw material for biocomposites. The cellulose content of OPF is 43%-65%, and the lignin content is 13%-25%. The composite filament consists of OPF (5%, mass fraction) in the ABS matrix. The fabrication procedure included alkalinizing, drying, and crushing the OPF to develop the composite. The OPF/ABS materials were prepared and completely blended to acquire a mix of 250 g of the material for the composition. Next, the FLD25 filament extrusion machine was used to form the OPF/ABS composite into a wire. This composite filament then was used in an FDM-based 3D printer to print the specimens. Finally, the printed specimens were tested for mechanical properties such as tensile and flexural strength. The results show that the presence of OPF had increased the tensile strength and modulus elasticity by approximately 1.9% and 1.05%, respectively. However, the flexural strength of the OPF/ABS composite had decreased by 90.6% compared with the virgin ABS. Lastly, the most significant outcome of the OPF/ABS composite was its suitability for printing using the FDM method.The full text can be downloaded at https://link.springer.com/content/pdf/10.1007%2Fs40436-019-00287-w.pdf     

Alternative for Rapid Detection and Screening of Pork,Chicken, and Beef Using Dielectric Properties in the Frequency of 0.5 to 50 GHz

Dielectric property at high microwave frequency region has been utilized for possible rapid detection and screening of different types of meat, especially for halal authentication. This investigation focused on both raw and sterilized (processed) beef, pork, and chicken samples. Dielectric response that consists of dielectric constant and dielectric loss factor was measured over the range of 0.5 to 50 GHz. All raw and sterilized meat samples could be differentiated by the dielectric values. Two distinct peaks were observed only for both raw and sterilized pork samples at the frequency around 7.43 and 31.19 GHz. These peaks can potentially be linked to compounds that exist only in pork such as DNA, microbes, enzyme, proteins, amino acids, and many others. Dielectric values for sterilized samples were lower than raw samples due to molecule structural changes that occurred in the samples. The dielectric results promise a great potential of utilizing dielectric properties as a rapid on-site detection approach prior to subsequent laborious analysis.     

Solid terpolymer electrolyte based on poly(vinyl butyral‐co‐vinyl alcohol‐co‐vinyl acetate) incorporated with lithium salt and tetraglyme for EDLCs

Solid terpolymer electrolytes (STEs) consist of different ratios of poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVBVA) and bis(trifluoromethane) sulfonamide lithium salt (LiTFSi) were prepared and the ionic conductivity of the prepared STEs was evaluated. The optimized STE (denoted as STE 20) was further doped with various amount of tetraglyme (10, 20, and 30 wt % and denoted as G10, G20, and G30, respectively). G20 enhanced the ionic conductivity from 6.22 (for STE 20) to 21.9 µS cm⁻¹. This enhancement is due to the presence of abundant oxygen-containing functional group in tetraglyme that provides more charge carrier mobility in the polymer matrix. The structure and complexation of the materials are authenticated via X-ray diffraction and Fourier transform infrared spectroscopy analysis. The performance of electric double layer capacitors based on activated carbon (AC) fabricated with STE 20 (AC/STE 20/AC) and G20 (AC/G 20/AC) were studied via cyclic voltammetry, galvanostatic charge–discharge, and electrochemical impedance spectroscopy. AC/G 20/AC achieved the maximum specific capacitance of 10.20 F/g [which is higher than AC/STE 20/AC (9.30 F/g)] with 75% of specific capacitance retention after 1500 cycles. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 45902.     

Physico-mechanical properties of particleboard made from heat-treated rubberwood particles

Physico-mechanical properties of particleboard fabricated from heat-treated rubberwood particles were investigated. Reduction in water absorption and mass loss were observed in heat-treated rubberwood particles, and it was associated with the properties of particleboard. The density and moisture content of the particleboard decreased with increasing treatment temperature and time. Heat treatment of particles improved the dimensional stability of the particleboard, but the mechanical properties were adversely affected.     

On the Particle Size Distribution of a Basaltic Till

Stress and strain history play a major role in defining the final grading of a soil, and by studying how the particle size distribution of a soil evolves with stress and strain it may be possible to highlight some important aspects of the sediment past history. In this paper, the characteristics and evolution of the grading of a till from Iceland, so-called Langjökull sediment, are examined. Specimens of the till were tested in compression (triaxial, oedometer tests) to very high pressures, and in shearing (triaxial, ring shear tests) to very high strains. The change in grading corresponding to particle breakage during compression and shearing was monitored using sieve analysis and scanning electron microscopy. The results show that the particle size distribution of the till, which is well graded, does not evolve when the till is subjected to high stresses or strains. Additional tests on specimens made of the larger-sized till particles did however show signs of breakage during testing. This suggests that the current natural grading of the till was achieved by incessant shearing during its past history, and corresponds to an ultimate, “critical” grading. This is confirmed by the mineralogical analysis of the sediment, which highlights a single parent rock and weathering of the particles by mechanical processes only.