Pectic Galactan Polysaccharide-Based Gene Delivery System for Targeting Neuroinflammation

Treating neuroinflammation-related injuries and disorders through manipulation of neuroinflammation functions is being heralded as a new therapeutic strategy. In this study, a novel pectic galactan (PG) polysaccharide based gene therapy approach is developed for targeting reactive gliosis in neuroinflammation. Galectin-3 (Gal-3) is a cell protein with a high affinity to β-galactoside sugars and is highly expressed in reactive gliosis. Since PG carries galactans, it can target reactive gliosis via specific carbohydrate interaction between galactan and Gal-3 on the cell membrane, and therefore can be utilized as a carrier for delivering genes to these cells. The carrier is synthesized by modifying quaternary ammonium groups on the PG. The resulting quaternized PG (QPG) is found to form complexes with plasmid DNA with a mean diameter of 100 nm and have the characteristics required for targeted gene therapy. The complexes efficiently condense large amounts of plasmid per particle and successfully bind to Gal-3. The in vivo study shows that the complexes are biocompatible and safe for administration and can selectively transfect reactive glial cells of an induced cortical lesion. The results confirm that this PG-based delivery system is a promising platform for targeting Gal-3 overexpressing neuroinflammation cells for treating neuroinflammation-related injuries and neurodegenerative diseases.     

Coupling of free surface and groundwater flows

In this paper we present some preliminary results about the coupling of shallow water equations for free surface flows and Darcy equation for groundwater flows. A suitable set of interface conditions is discussed: the Beavers and Joseph formula for the bottom stress is used. An iterative algorithm to solve the coupled problem is proposed and some numerical results are presented.     

Approximate similarity retrieval with M-trees

Motivated by the urgent need to improve the efficiency of similarity queries, approximate similarity retrieval is investigated in the environment of a metric tree index called the M-tree. Three different approximation techniques are proposed, which show how to forsake query precision for improved performance. Measures are defined that can quantify the improvements in performance efficiency and the quality of approximations. The proposed approximation techniques are then tested on various synthetic and real-life files. The evidence obtained from the experiments confirms our hypothesis that a high-quality approximated similarity search can be performed at a much lower cost than that needed to obtain the exact results. The proposed approximation techniques are scalable and appear to be independent of the metric used. Extensions of these techniques to the environments of other similarity search indexes are also discussed. Received July 7, 1998 / Accepted October 13, 1998     

Tropos: An Agent-Oriented Software Development Methodology

Our goal in this paper is to introduce and motivate a methodology, called Tropos,¹ for building agent oriented software systems. Tropos is based on two key ideas. First, the notion of agent and all related mentalistic notions (for instance goals and plans) are used in all phases of software development, from early analysis down to the actual implementation. Second, Tropos covers also the very early phases of requirements analysis, thus allowing for a deeper understanding of the environment where the software must operate, and of the kind of interactions that should occur between software and human agents. The methodology is illustrated with the help of a case study. The Tropos language for conceptual modeling is formalized in a metamodel described with a set of UML class diagrams.     

Are electronic white canes better than traditional canes? A comparative study with blind and blindfolded participants

Visually impaired individuals often rely on assistive technologies such as white canes for independent navigation. Many electronic enhancements to the traditional white cane have been proposed. However, only a few of these proof-of-concept technologies have been tested with authentic users, as most studies rely on blindfolded non-visually impaired participants or no testing with participants at all. Experiments involving blind users are usually not contrasted with the traditional white cane. This study set out to compare an ultrasound-based electronic cane with a traditional white cane. Moreover, we also compared the performance of a group of visually impaired participants (N = 10) with a group of blindfolded participants without visual impairments (N = 31). The results show that walking speed with the electronic cane is significantly slower compared to the traditional white cane. Moreover, the results show that the performance of the participants without visual impairments is significantly slower than for the visually impaired participants. No significant differences in obstacle detection rates were observed across participant groups and device types for obstacles on the ground, while 79% of the hanging obstacles were detected by the electronic cane. The results of this study thus suggest that electronic canes present only one advantage over the traditional cane, namely in its ability to detect hanging obstacles, at least without prolonged practice. Next, blindfolded participants are insufficient substitutes for blind participants who are expert cane users. The implication of this study is that research into digital white cane enhancements should include blind participants. These participants should be followed over time in longitudinal experiments to document if practice will lead to improvements that surpass the performance achieved with traditional canes.

     

Adsorption behavior and kinetics of H2S on a potassium-promoted hydrotalcite

Adsorption of H₂S and the influence of steam on its adsorption capacity and kinetics were studied on a commercial potassium-promoted hydrotalcite. The sorbent shows a very high cyclic working capacity for H₂S compared to CO₂ and H₂O, even at lower partial pressures and at different operating temperatures ranging between 300 and 500 °C. The operating temperature does not significantly influence the cyclic working capacity for half-cycle times of 30 min. The adsorption mechanism, however, changes at higher temperatures. At lower temperatures (300 °C) a fast adsorption with a fast approach to steady state was observed. At higher operating temperatures, H₂S reacts with the hydrotalcite structure, forming strongly bonded sulfuric species on the sorbent. When using dry regeneration conditions, the first cycles in cyclic operation at higher temperatures show a significantly higher adsorption of H₂S (especially the first cycle), which cannot be desorbed during regeneration with N₂. After the first fast initial adsorption rate a continuous slow adsorption of H₂S occurs, probably caused by a surface reaction between H₂S and the hydrotalcite structure. This reaction is, however, reversible if steam is used.The adsorption mechanism for H₂S and H₂O was determined using multiple cyclic experiments comparable to previous studies performed for CO₂ and H₂O adsorption. It is evident that the adsorption mechanism developed for CO₂ on the same sorbents is also valid for H₂S, indicating that the developed mechanism is consistent for sour gas adsorption on this type of sorbents. The cyclic working capacity can be significantly increased if steam is used during the regeneration step of the sorbent. The mechanistic model developed for the adsorption of CO₂ and H₂O was successfully validated with more than 160 different TGA experiments. An operating temperature of 400 °C seems to be optimal to achieve a high cyclic working capacity for H₂S, because at higher temperatures the regeneration of the formed sulfuric species seems to be hindered resulting in a significant decrease in the cyclic working capacity.     

Polyacrylamide-gelatin polymeric networks: effect of pH and gelatin concentration on the swelling kinetics and mechanical properties

This work presents the synthesis of polyacrylamide-gelatin (PAM-G) semi-interpenetrating hydrogels, as well as the study of the swelling capacity of this material at different pH’s, and we report its Young modulus. The hydrogels were crosslinked with N,N′-methylenebisacrylamide and synthesized at different acrylamide/gelatin weight relationship. It was observed that the swelling capacity of the hydrogels increases when the gelatin concentration is increased; while the Young modulus (at the swelling equilibrium) decreases lightly. Therefore, the gelatin has a small effect in the Young modulus, unlike its influence in the swelling ability. The swelling experiments reveal that the PAM-G hydrogels increase its swelling capacity in alkaline mediums because the presence of the hydrophobic functional groups (mainly COO⁻) in the gelatin structure.