全文获取类型
收费全文 | 795篇 |
免费 | 67篇 |
专业分类
电工技术 | 4篇 |
化学工业 | 403篇 |
金属工艺 | 6篇 |
机械仪表 | 16篇 |
建筑科学 | 26篇 |
能源动力 | 13篇 |
轻工业 | 143篇 |
无线电 | 39篇 |
一般工业技术 | 90篇 |
冶金工业 | 42篇 |
原子能技术 | 1篇 |
自动化技术 | 79篇 |
出版年
2024年 | 1篇 |
2023年 | 12篇 |
2022年 | 128篇 |
2021年 | 104篇 |
2020年 | 29篇 |
2019年 | 29篇 |
2018年 | 42篇 |
2017年 | 27篇 |
2016年 | 36篇 |
2015年 | 42篇 |
2014年 | 44篇 |
2013年 | 47篇 |
2012年 | 39篇 |
2011年 | 47篇 |
2010年 | 33篇 |
2009年 | 35篇 |
2008年 | 28篇 |
2007年 | 13篇 |
2006年 | 18篇 |
2005年 | 26篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 4篇 |
1999年 | 6篇 |
1998年 | 9篇 |
1997年 | 8篇 |
1996年 | 11篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1977年 | 2篇 |
1976年 | 2篇 |
排序方式: 共有862条查询结果,搜索用时 15 毫秒
1.
Adele Boccuto Filippo Dragoni Francesca Picarazzi Alessia Lai Carla Della Ventura Carla Veo Federica Giammarino Francesco Saladini Gianguglielmo Zehender Maurizio Zazzi Mattia Mori Ilaria Vicenti 《International journal of molecular sciences》2021,22(5)
The nucleotide analog sofosbuvir, licensed for the treatment of hepatitis C, recently revealed activity against the Zika virus (ZIKV) in vitro and in animal models. However, the ZIKV genetic barrier to sofosbuvir has not yet been characterized. In this study, in vitro selection experiments were performed in infected human hepatoma cell lines. Increasing drug pressure significantly delayed viral breakthrough (p = 0.029). A double mutant in the NS5 gene (V360L/V607I) emerged in 3 independent experiments at 40–80 µM sofosbuvir resulting in a 3.9 ± 0.9-fold half- maximal inhibitory concentration (IC50) shift with respect to the wild type (WT) virus. A triple mutant (C269Y/V360L/V607I), detected in one experiment at 80 µM, conferred a 6.8-fold IC50 shift with respect to the WT. Molecular dynamics simulations confirmed that the double mutant V360L/V607I impacts the binding mode of sofosbuvir, supporting its role in sofosbuvir resistance. Due to the distance from the catalytic site and to the lack of reliable structural data, the contribution of C269Y was not investigated in silico. By a combination of sequence analysis, phenotypic susceptibility testing, and molecular modeling, we characterized a double ZIKV NS5 mutant with decreased sofosbuvir susceptibility. These data add important information to the profile of sofosbuvir as a possible lead for anti-ZIKV drug development. 相似文献
2.
3.
Sebastian Reiter Dmitry Logashenko Alfio Grillo Gabriel Wittum 《Computing and Visualization in Science》2012,15(4):209-225
This work presents an extension of grid generation techniques for finite-volume discretizations of density-driven flow in fractured porous media, in which fractures are considered as low-dimensional manifolds and are resolved by sides of grid elements. The proposed technique introduces additional degrees of freedom for the unknowns assigned to the fractures and thus allows to reconstruct jumps of the solution over a fracture. Through the concept of degenerated elements, the proposed technique can be used for arbitrary junctions of fractures but is sufficiently simple regarding the implementation and allows for the application of conventional numerical solvers. Numerical experiments presented at the end of the paper demonstrate the applicability of this technique in two and three dimensions for complicated fracture networks. 相似文献
4.
Felisari L Grillo V Jabeen F Rubini S Menozzi C Rossi F Martelli F 《Ultramicroscopy》2011,111(8):1018-1028
A dedicated specimen holder has been designed to perform low-voltage scanning transmission electron microscopy in dark field mode. Different test samples, namely InGaAs/GaAs quantum wells, InGaAs nanowires and thick InGaAs layers, have been analysed to test the reliability of the model based on the proportionality to the specimen mass-thickness, generally used for image intensity interpretation of scattering contrast processes. We found that size of the probe, absorption and channelling must be taken into account to give a quantitative interpretation of image intensity. We develop a simple procedure to evaluate the probe-size effect and to obtain a quantitative indication of the absorption coefficient. Possible artefacts induced by channelling are pointed out. With the developed procedure, the low voltage approach can be successfully applied for quantitative compositional analysis. The method is then applied to the estimation of the In content in the core of InGaAs/GaAs core-shell nanowires. 相似文献
5.
Giulia RabottinoAuthor Vitae Federica CaselliAuthor Vitae 《Computer Standards & Interfaces》2011,33(2):128-135
At present, mammography is the most effective examination for an early diagnosis of breast cancer. Nevertheless, the detection of cancer signs in mammograms is a difficult procedure owing to the great number of non-pathological structures which are also present in the image. Recent statistics show that in current breast cancer screenings 10%-25% of the tumors are missed by the radiologists. For this reason, a lot of research is currently being done to develop systems for Computer Aided Detection (CADe). Probably, some causes of the false-negative screening examinations are that tumoral masses have varying dimension and irregular shape, their borders are often ill-defined and their contrast is very low, thus making difficult the discrimination from parenchymal structures. Therefore, in a CADe system a preliminary segmentation procedure has to be implemented in order to separate the mass from the background tissue. In this way, various characteristics of the segmented mass can be evaluated and used in a classification step to discriminate benign and malignant cases. In this paper, we describe an effective algorithm for massive lesions segmentation based on a region-growing technique and we provide full details the performance evaluation procedure used in this specific context. 相似文献
6.
Federica Cena 《Journal of Intelligent Information Systems》2011,36(2):131-166
Nowadays there is a great number of Web information systems that build a model of the user and adapt their services according
to the needs and preferences maintained by the user model (UM). One of the most challenging issues of this scenario is the
possibility to enable different systems to cooperate in order to exchange the available information about a user. Our aim
is to create rich (and scalable) communication protocols and infrastructures to enable consumers and providers of UM data
to interact. Our solution for dealing with such an issue is to exploit Web standards for interoperability (i.e. Semantic Web
and Web Services) for implementing simple atomic communication, and a dialogue model for implementing enhanced communication
capabilities. In particular, two systems can start a semantics-enhanced Dialogue Game as a form of negotiation to clarify
the meaning of the requested concepts when a shared knowledge model does not exist, and to approximate the response when the
exact one is not available. We propose a distributed semantic conversation framework based on the Sesame semantic environment
for the exchange of user model knowledge on the Web. Systems have to expose their user model data as a Web Service, and to
exploit a public dialogue knowledge base to start the dialogue. The main advantage of the approach is to allow systems to
deal with difficult situations by starting an appropriate dialogue game instead of stopping the communication as in the traditional
“all-or-nothing” Web Service approach. On the basis of a preliminary evaluation, the approach has shown an improvement of
the adaptation results provided by the systems we tested. 相似文献
7.
In this work we present Bio-PEPA, a process algebra for the modelling and the analysis of biochemical networks. It is a modification of PEPA, originally defined for the performance analysis of computer systems, in order to handle some features of biological models, such as stoichiometry and the use of general kinetic laws. Bio-PEPA may be seen as an intermediate, formal, compositional representation of biological systems, on which different kinds of analyses can be carried out. Bio-PEPA is enriched with some notions of equivalence. Specifically, the isomorphism and strong bisimulation for PEPA have been considered and extended to our language. Finally, we show the translation of a biological model into the new language and we report some analysis results. 相似文献
8.
Julia Andreeva Simone Campana Federica Fanzago Juha Herrala 《Journal of Grid Computing》2008,6(1):3-13
In this paper we present the experience of the ATLAS and CMS High-Energy Physics (HEP) experiments at the Large Hadron Collider
(LHC) with the LCG/EGEE Grid infrastructure. The activity developed around the following two main lines: large-scale physics
and detector simulations and end-user analysis. The LCG/EGEE Grid infrastructure offers a large amount of computing and storage
resources and is growing very rapidly. It provides the natural environment for large-scale physics and detector simulations.
Also, the analysis of these detector simulation data (and in the near future of the reconstructed data from physics collisions)
requires efficient end-users access to Grid resources. In this paper, the main findings and lessons learned in terms of performance,
robustness and scalability of the whole system are discussed in detail. 相似文献
9.
Laura Mannarino Lara Paracchini Federica Pezzuto Gheorghe Emilian Olteanu Laura Moracci Luca Vedovelli Irene De Simone Cristina Bosetti Monica Lupi Rosy Amodeo Alessia Inglesi Maurizio Callari Serena Penpa Roberta Libener Sara Delfanti Antonina De Angelis Alberto Muzio Paolo Andrea Zucali Paola Allavena Giovanni Luca Ceresoli Sergio Marchini Fiorella Calabrese Maurizio DIncalci Federica Grosso 《International journal of molecular sciences》2022,23(10)
10.
Gianmaria Salvio Alessandro Ciarloni Melissa Cutini Nicola delli Muti Federica Finocchi Michele Perrone Silvia Rossi Giancarlo Balercia 《International journal of molecular sciences》2022,23(10)
Metabolic syndrome (MetS) is a highly prevalent condition among adult males, affecting up to 41% of men in Europe. It is characterized by the association of obesity, hypertension, and atherogenic dyslipidemia, which lead to premature morbidity and mortality due to cardiovascular disease (CVD). Male infertility is another common condition which accounts for about 50% of cases of couple infertility worldwide. Interestingly, male infertility and MetS shares several risk factors (e.g., smoking, ageing, physical inactivity, and excessive alcohol consumption), leading to reactive oxygen species (ROS) production and increased oxidative stress (OS), and resulting in endothelial dysfunction and altered semen quality. Thus, the present narrative review aims to discuss the pathophysiological mechanisms which link male infertility and MetS and to investigate the latest available evidence on the reproductive consequences of MetS. 相似文献