Chemoenzymatic Asymmetric Synthesis of Pyridine-Based α-Fluorinated Secondary Alcohols

Fluoro-substituted and heteroaromatic compounds are valuable intermediates for a variety of applications in pharma- and agrochemistry and synthetic chemistry. This study investigates the chemoenzymatic preparation of chiral alcohols bearing a heteroaromatic ring with an increasing degree of fluorination in α-position. Starting from readily available picoline derivatives prochiral α-halogenated acyl moieties were introduced with excellent selectivity and 64–95 % yield. The formed carbonyl group was subsequently reduced to the corresponding alcohols using the alcohol dehydrogenase from Lactobacillus kefir, yielding an enantiomeric excess of 95–>99 % and up to 98 % yield.     

What Can We Learn from FGF-2 Isoform-Specific Mouse Mutants? Differential Insights into FGF-2 Physiology In Vivo

Fibroblast growth factor 2 (FGF-2), ubiquitously expressed in humans and mice, is functionally involved in cell growth, migration and maturation in vitro and in vivo. Based on the same mRNA, an 18-kilo Dalton (kDa) FGF-2 isoform named FGF-2 low molecular weight (FGF-2LMW) isoform is translated in humans and rodents. Additionally, two larger isoforms weighing 21 and 22 kDa also exist, summarized as the FGF-2 high molecular weight (FGF-2HMW) isoform. Meanwhile, the human FGF-2HMW comprises a 22, 23, 24 and 34 kDa protein. Independent studies verified a specific intracellular localization, mode of action and tissue-specific spatiotemporal expression of the FGF-2 isoforms, increasing the complexity of their physiological and pathophysiological roles. In order to analyze their spectrum of effects, FGF-2LMW knock out (ko) and FGF-2HMWko mice have been generated, as well as mice specifically overexpressing either FGF-2LMW or FGF-2HMW. So far, the development and functionality of the cardiovascular system, bone formation and regeneration as well as their impact on the central nervous system including disease models of neurodegeneration, have been examined. This review provides a summary of the studies characterizing the in vivo effects modulated by the FGF-2 isoforms and, thus, offers a comprehensive overview of its actions in the aforementioned organ systems.     

Deliberately Losing Control of C−H Activation Processes in the Design of Small-Molecule-Fragment Arrays Targeting Peroxisomal Metabolism

Combined photochemical arylation, “nuisance effect” (S_NAr) reaction sequences have been employed in the design of small arrays for immediate deployment in medium-throughput X-ray protein–ligand structure determination. Reactions were deliberately allowed to run “out of control” in terms of selectivity; for example the ortho-arylation of 2-phenylpyridine gave five products resulting from mono- and bisarylations combined with S_NAr processes. As a result, a number of crystallographic hits against NUDT7, a key peroxisomal CoA ester hydrolase, have been identified.     

The Two-Dimensional Clifford-Fourier Transform

Recently several generalizations to higher dimension of the Fourier transform using Clifford algebra have been introduced, including the Clifford-Fourier transform by the authors, defined as an operator exponential with a Clifford algebra-valued kernel. In this paper an overview is given of all these generalizations and an in depth study of the two-dimensional Clifford-Fourier transform of the authors is presented. In this special two-dimensional case a closed form for the integral kernel may be obtained, leading to further properties, both in the L ₁ and in the L ₂ context. Furthermore, based on this Clifford-Fourier transform Clifford-Gabor filters are introduced. AMS subject classification numbers: 42B10, 30G35 Fred Brackx received a diploma degree in mathematics from Ghent University, Belgium, in 1970 and a Ph.D. degree in mathematics from the same university in 1973. Since 1984 he is professor for mathematical analysis at Ghent University and currently he is leading the Clifford Research Group. His main interests are function theory and functional analysis for functions with values in quaternion and Clifford algebras. The research covers Clifford distributions, generalized Fourier, Radon and Hilbert transforms, orthogonal polynomials and multi-dimensional wavelets. Nele De Schepper received a diploma degree in mathematics from Ghent University, Belgium, in 2001. Since then she holds an assistantship at the Department of Mathematical Analysis of Ghent University and is a member of the Clifford Research Group. Her main interests are function theory and functional analysis for functions with values in Clifford algebras. The research covers generalized Fourier transforms, orthogonal polynomials and multi-dimensional wavelets. Frank Sommen received a diploma degree in mathematics from Ghent University, Belgium, in 1978, a Ph.D. degree in mathematics from the same university in 1980, and a habilitation degree in mathematical analysis in 1984. From 1978 until 1999 he was at the National Fund for Scientific Research (Flanders). Since 2000 he holds a Research professorship at Ghent University. His main interests are function theory and functional analysis for functions with values in quaternion and Clifford algebras. The research covers Clifford distributions, generalized Fourier, Radon and Hilbert transforms, orthogonal polynomials and multi-dimensional wavelets, algebraic analysis, hyperfunctions and radial algebra.     

Implementation of RNS-Based Distributed Arithmetic Discrete Wavelet Transform Architectures Using Field-Programmable Logic

Currently there are design barriers inhibiting the implementation of high-precision digital signal processing (DSP) objects with field programmable logic (FPL) devices. This paper explores overcoming these barriers by fusing together the popular distributed arithmetic (DA) method with the residue number system (RNS) for use in FPL-centric designs. The new design paradigm is studied in the context of a high-performance filter bank and a discrete wavelet transform (DWT). The proposed design paradigm is facilitated by a new RNS accumulator structure based on a carry save adder (CSA). The reported methodology also introduces a polyphase filter structure that results in a reduced look-up table (LUT) budget. The 2C-DA and RNS-DA are compared, in the context of a FPL implementation strategy, using a discrete wavelet transform (DWT) filter bank as a common design theme. The results show that the RNS-DA, compared to a traditional 2C-DA design, enjoys a performance advantage that increases with precision (wordlength).     

Test Case Generation as an AI Planning Problem

While Artificial Intelligence techniques have been applied to a variety of software engineering applications, the area of automated software testing remains largely unexplored. Yet, test cases for certain types of systems (e.g., those with command language interfaces and transaction based systems) are similar to plans. We have exploited this similarity by constructing an automated test case generator with an AI planning system at its core. We compared the functionality and output of two systems, one based on Software Engineering techniques and the other on planning, for a real application: the StorageTek robot tape library command language. From this, we showed that AI planning is a viable technique for test case generation and that the two approaches are complementary in their capabilities.     

Comment and discussion on digital processing of PD pulses

Some of the more salient aspects of the digital processing technology of PD signals are examined. Most of the efforts in this field are concentrated on the application of digital analyzers for pulse height analysis, pattern recognition and identification of the physical phenomena. It is demonstrated that errors in the signal processing unit can lead to dominant mistakes in the interpretation of the test results