Cerebral microbleeds and cognitive decline in a hemodialysis patient: Case report and review of literature

Cerebral microbleeds (CMBs) are small hemosiderin deposits indicative of prior cerebral microscopic hemorrhage and previously thought to be clinically silent. Recent population‐based cross‐sectional studies and prospective longitudinal cohort studies have revealed association between CMB and cognitive dysfunction. In the general population, CMBs are associated with age, hypertension, and cerebral amyloid angiopathy. In the chronic kidney disease (CKD) population, diminished estimated glomerular filtration rate has been found to be an independent risk factor for CMB, raising the possibility that a uremic milieu may predispose to microbleeds. In the end‐stage renal disease (ESRD) population on hemodialysis, the incidence of microbleeds is significantly higher compared with a control group without history of CKD or stroke. We present an ESRD patient on chronic hemodialysis with a history of gradual cognitive decline and progressive CMBs. Through this case and literature review, we illustrate the need to develop detection and prediction models to treat this frequent development in ESRD patients.     

Inactivation of Staphylococcus hyicus lipase by hexadecylsulfonyl fluoride: evidence for an active site serine

The Staphylococcus hyicus lipase is an acyl hydrolase with broadsubstrate specificity including neutral glycerides and phospholipids.To obtain further insight into the mechanism of action of thisenzyme, we tested several sulfonyl fluorides as active site-directedinhibitors. The enzyme is resistant to the well-known serineprotease/esterase inhibitor phenylmethanesulfonyl fluoride (PMSF),but is rapidly inactivated by hexadecylsulfonyl fluoride. Thekinetics of inactivation were studied in Triton X-100 micelles.Inactivation is fast and the rate of inactivation is constantover the pH range where this lipase is active. Metal ions likeCa²⁺ and Sr²⁺ do not appreciably influence the rate of inactivation,although the enzymatic activity is significantly increased,suggesting a structural role for these ions. The S.hyicus lipasecontains a consensus sequence G-H/Y-S-X-G. Substitution by site-directedmutagenesis of this serine (Ser369) by a cysteine resulted ina mutant with only 0.2% residual activity. The activity of thismutant could not be inhibited with water-soluble sulfhydrylreagents either in the presence or absence of Triton X-100 micelles.In the presence of Triton X-100 micelles, inactivation of themutant occurred with 4-nitrophenylhexadecyl disulfide (t_1/2= 125 min) while the wild-type enzyme does not react at all.We conclude that Ser369 is the active site residue and thatin water this residue is inaccessible. Only after interfacialactivation Ser369 (or Cys369) becomes exposed and reacts withirreversible inhibitors.     

Development and Performance of Crystallization with Additives Applied on Different Milk Samples

Crystallization with additives is developed on milk samples from different processing treatments. Performance tests are carried out based on structure analysis of the crystallization patterns. Crystallization with milk as additive is applied following changes in milk after different processing treatments. When an aqueous cupric chloride dihydrate solution crystallizes in the presence of milk as additive, specific patterns emerge, which can be evaluated by image analysis. Milk samples were heated and homogenized in a pilot plant and characterized by various parameters. Furthermore, milk samples from the market were tested. Patterns from milk after heat treatment and homogenization are significantly different from those derived from untreated milk. The experiments could be reproduced for other milk samples, on different days and in another laboratory.     

Security requirement analysis of business processes

Economic globalization leads to complex decentralized company structures calling for the extensive use of distributed IT-systems. The business processes of a company have to reflect these changes of infrastructure. In particular, due to new electronic applications and the inclusion of a higher number of—potentially unknown—persons, the business processes are more vulnerable against malicious attacks than traditional processes. Thus, a business should undergo a security analysis. Here, the vulnerabilities of the business process are recognized, the risks resulting from the vulnerabilities are calculated, and suitable safeguards reducing the vulnerabilities are selected. Unfortunately, a security analysis tends to be complex and affords expensive security expert support. In order to reduce the expense and to enable domain experts with in-depth insight in business processes but with limited knowledge about security to develop secure business processes, we developed the framework MoSS_BP facilitating the handling of business process security requirements from their specification to their realization. In particular, MoSS _BP provides graphical concepts to specify security requirements, repositories of various mechanisms enforcing the security requirements, and a collection of reference models and case studies enabling the modification of the business processes. In this paper, the MoSS _BP -framework is presented. Additionally, we introduce a tool supporting the MoSS_BP-related security analysis of business processes and the incorporation of safeguards. This tool is based on object-oriented process models and acts with graph rewrite systems. Finally, we clarify the application of the MoSS_BP-framework by means of a business process for tender-handling which is provided by anonymity-preserving safeguards. Peter Herrmann studied computer science at the University of Karlsruhe, Germany (diploma in 1990). Afterwards, he worked as a Ph.D. student (doctorate in 1997) and postdoctoral researcher in the Computer Networks and Distributed Systems Group of the Computer Science Department at the University of Dortmund, Germany. Since 2005 he is a full professor for formal methods at the Department for Telematics of the Norwegian University of Science and Technology (NTNU) in Trondheim, Norway. His research interests include the formal-based development of networked systems and the engineering of distributed services. Moreover, he is interested in security and trust aspects of component structured distributed software. Gaby Herrmann studied computer science at the University of Karlsruhe, Germany (diploma in 1991). Afterwards, she worked as a researcher in the Communication Group and the Information Systems Group at University of Duisburg-Essen (Doctorate in 2001, topic: security of business processes). Since 2000 she works as executive secretary at the Department of Economics, Business Studies and Computer Sciences at the same university.     

Citation analysis and peer ranking of Australian social science journals

Citation analyses were performed for Australian social science journals to determine the differences between data drawn from Web of Science and Scopus. These data were compared with the tier rankings assigned by disciplinary groups to the journals for the purposes of a new research assessment model, Excellence in Research for Australia (ERA), due to be implemented in 2010. In addition, citation-based indicators including an extended journal impact factor, the h-index, and a modified journal diffusion factor, were calculated to assess whether subsequent analyses influence the ranking of journals. The findings suggest that the Scopus database provides higher number of citations for more of the journals. However, there appears to be very little association between the assigned tier ranking of journals and their rank derived from citations data. The implications for Australian social science researchers are discussed in relation to the use of citation analysis in the ERA.     

光刻探头的共振压力显微系统的研究

以磁共振压力显微和半导体核子自旋态研究为目的,采用电子束和等离子体刻印方法制备硅振荡器,脉冲调制序列控制磁共振条件频率和旋转框架翻转,依据样品的自旋-晶格弛豫时间和自旋-自旋弛豫时间,获得了共振显微压力,测试了压力显微的灵敏度。结果表明,光刻的探头具有高Q值和软悬臂低倔强系数K,磁共振压力通过扫描片段和激光光纤干涉得到了极其微小的压力。光刻探头的共振压力显微具有高空间分辨率,兼具核磁共振成像(MRI)和原子压力显微技术(AFM)优点,是一种重要的核自旋探测技术和三维原子分辨率成像的有力方法。     

Continuous tracheal gas insufflation enables a volume reduction strategy in hyaline membrane disease: technical aspects and clinical results

OBJECTIVE: Instrumental dead space wash-out can be used to improve carbon dioxide clearance. The aim of this study was to define, using a bench test, an optimal protocol for long-term use, and to assess the efficacy of this technique in neonates. DESIGN: A bench test with an artificial lung model, and an observational prospective study. Dead space wash-out was performed by continuous tracheal gas insufflation (CTGI), via six capillaries molded in the wall of a specially designed endotracheal tube, in 30 preterm neonates with hyaline membrane disease. SETTING: Neonatal intensive care unit of a regional hospital. RESULTS: The bench test study showed that a CTGI flow of 0.5 l/ min had the optimal efficacy-to-side-effect ratio, resulting in a maximal or submaximal efficacy (93 to 100%) without a marked increase in tracheal and CTGI circuit pressures. In the 30 newborns, 15 min of CTGI induced a significant fall in arterial carbon dioxide tension (PaCO2), from 45 +/- 7 to 35 +/- 5 mmHg (p = 0.0001), and in 14 patients allowed a reduction in the gradient between Peack inspirating pressure and positive end-expiratory pressure from 20.8 +/- 4.6 to 14.4 +/- 3.7 cmH2O (p < 0.0001) while keeping the transcutaneous partial pressure of carbon dioxide constant. As predicted by the bench test, the decrease in PaCO2 induced by CTGI correlated well with PaCO2 values before CTGI (r = 0.58, p < 0.002) and with instrumental dead space-to-tidal volume ratio (r = 0.54, p < 0.005). CONCLUSION: CTGI may be a useful adjunct to conventional ventilation in preterm neonates with respiratory disease, enabling an increase in CO2 clearance or a reduction in ventilatory pressure.