Near-Optimal Signal Preprocessor for Positive Cardiac Arrhythmia Identification

Extremely high reliability of waveform detection is fundamental for computer-assisted identification of cardiac arrhythmias. The problem is formulated in terms of one of the basic aspects in digital signal processing, namely, detection of an unknown deterministic signal in noise. The signal is considered band limited and the noise as Gaussian with zero mean. The theory presented leads to an energy based detector. No a priori assumption is made about the morphology of the waveforms to be detected; only energy thresholding is necessary for event detection. The addition of a linear filter as a front end to the energy detector has resulted in an entire detection process described as suboptimal matched nonlinear filtering. The SNR gain at the input of the energy detector has given performances almost identical to the matched filter. The method has been implemented with an original fast algorithm to allow reasonable execution time for the processing of continuous long-duration signals (1 h). The design of such a fillter is described along with its application to the processing of two simultaneously recorded cardiac signals. The two signals are an auricular electrogram (OEG from an esophageal pill electrode) and an ECG (lead II or III). Systematic evaluation and detection performance results show that the proposed method could be seriously considered as a near-optimal approach to waveform detection, since it is based on proven signal processing theory and is far superior to heuristic methods.     

Refining Genotypes and Phenotypes in KCNA2-Related Neurological Disorders

Pathogenic variants in KCNA2, encoding for the voltage-gated potassium channel K_v1.2, have been identified as the cause for an evolving spectrum of neurological disorders. Affected individuals show early-onset developmental and epileptic encephalopathy, intellectual disability, and movement disorders resulting from cerebellar dysfunction. In addition, individuals with a milder course of epilepsy, complicated hereditary spastic paraplegia, and episodic ataxia have been reported. By analyzing phenotypic, functional, and genetic data from published reports and novel cases, we refine and further delineate phenotypic as well as functional subgroups of KCNA2-associated disorders. Carriers of variants, leading to complex and mixed channel dysfunction that are associated with a gain- and loss-of-potassium conductance, more often show early developmental abnormalities and an earlier onset of epilepsy compared to individuals with variants resulting in loss- or gain-of-function. We describe seven additional individuals harboring three known and the novel KCNA2 variants p.(Pro407Ala) and p.(Tyr417Cys). The location of variants reported here highlights the importance of the proline(405)–valine(406)–proline(407) (PVP) motif in transmembrane domain S6 as a mutational hotspot. A novel case of self-limited infantile seizures suggests a continuous clinical spectrum of KCNA2-related disorders. Our study provides further insights into the clinical spectrum, genotype–phenotype correlation, variability, and predicted functional impact of KCNA2 variants.     

Variation in Cyanogenic Glycosides Across Populations of Wild Lima Beans (Phaseolus lunatus) Has No Apparent Effect on Bruchid Beetle Performance

Cyanogenic glycosides (CNGs) act as feeding or oviposition deterrents and are toxic after enzymatic hydrolysis, thus negatively affecting herbivore performance. While most studies on CNGs focus on leaf herbivores, here we examined seeds from natural populations of Phaseolus lunatus in Mexico. The predominant CNGs, linamarin and lotaustralin, were quantified for each population by using ultra-high pressure liquid chromatography-mass spectrometry. We also examined whether there was a correlation between the concentration of CNGs and the performance of the Mexican bean beetle, Zabrotes subfasciatus, on seeds from each population^. The concentrations of CNGs in the seeds were relatively high compared to the leaves and were significantly variable among populations. Surprisingly, this had little effect on the performance of the bruchid beetles. Zabrotes subfasciatus can tolerate high concentrations of CNGs, most likely because of the limited β-glucosidase activity in the seeds. Seed herbivory does not appear to liberate hydrogen cyanide due to the low water content in the seed. This study illustrates the importance of quantifying the natural variation and activity of toxic compounds in order to make relevant biological inferences about their role in defense against herbivores.     

Streptococcus mitis cell walls and lipopolysaccharide induce lethality in D-galactosamine-sensitized mice by a tumor necrosis factor-dependent pathway

PURPOSE: To investigate the clonal origin of malignant cells in recurrent superficial bladder cancer. MATERIALS AND METHODS: We compared the protein expression of p53 and retinoblastoma (Rb) by immunohistochemistry using antibody P1801 and PMG3-245, respectively, in 13 patients at the time of primary superficial bladder cancer resection (6 Ta and 7 T1) and their 15 corresponding recurrences of disease. Mutations in p53 and Rb were inferred on the basis of immunoperoxidase staining. RESULTS: At the time of initial tumor resection, a p53 mutation was observed in 5 patients (39%) and an Rb mutation was observed in 3 (23%). The p53/Rb mutation status of recurrent bladder cancers completely matched their corresponding primary bladder cancer. CONCLUSIONS: The chance that recurrent bladder cancer originated from independent clones in this study was extremely small (p < 10(-6)). This result strongly supports the monoclonal origin of recurrent superficial bladder cancer.     

Subset ARMA Model Identification Using Genetic Algorithms

Subset models are often useful in the analysis of stationary time series. Although subset autoregressive models have received a lot of attention, the same attention has not been given to subset autoregressive moving-average (ARMA) models, as their identification can be computationally cumbersome. In this paper we propose to overcome this disadvantage by employing a genetic algorithm. After encoding each ARMA model as a binary string, the iterative algorithm attempts to mimic the natural evolution of the population of such strings by allowing strings to reproduce, creating new models that compete for survival in the next population. The success of the proposed procedure is illustrated by showing its efficiency in identifying the true model for simulated data. An application to real data is also considered.     

Aquifex aeolicus membrane hydrogenase for hydrogen biooxidation: Role of lipids and physiological partners in enzyme stability and activity

Hydrogenase I from the hyperthermophilic bacterium Aquifex aeolicus is a good candidate for biotechnological devices thanks to its ability to oxidize hydrogen at high temperature, even in the presence of oxygen and CO. In order to enhance the enzyme stability and the catalytic efficiency, we investigated the hydrogen oxidation process with hydrogenase I embedded in a physiological-like environment. Hydrogenase I partners in the metabolic chain, namely membrane quinone and cytochrome b, were purified and fully characterized. The complex hydrogenase I–cytochrome b was inserted into liposomes. Surface Plasmon Resonance revealed that quinone took part in the stabilization of the complex. By use of molecular modelization and electrochemistry analysis, enzyme stability has been demonstrated to be stronger and enzymatic efficiency to be five times higher when hydrogenase is embedded into the liposomes. This result raises the possibility of using hydrogenases as biocatalysts in fuel cells.     

Rubber band ligation of hemorrhoids: relapse as a function of time

Rubber band ligation is a well established treatment of internal second-degree hemorrhoids. Up to now, the long-term results (> 5 years) of this method have not been assessed. The aim of this study was to determine the long-term success rate of rubber band ligation in patients with a first episode of second-degree hemorrhoids not responding to medical treatment (local cream with laxative). A total of 92 patients who were treated between 1979 and 1997 for second-degree hemorrhoids responded to a questionnaire. The need for complementary conservative (sclerotherapy, cryotherapy or rubber band ligation) or surgical treatment was considered indicative of relapse. The collected data were analyzed according to the Kaplan-Meier method. The average follow-up time was 5.6 years. There were 21 patients who suffered a relapse. The probability of successful treatment was 0.77 (range: 0.67-0.88) after 5 years and 0.68 (range: 0.5-0.86) after 10.6 years. We conclude that rubber band ligation represent an efficient long-term treatment for second-degree hemorrhoids. Approximately two thirds of the patients are cured after 5 years and more than a half after 10 years.     

Study of active millimeter-wave image speckle reduction by Hadamard phase pattern illumination

Active millimeter-wave images typically exhibit characteristic speckle noise, due to the coherence of artificial millimeter-wave sources. We study the Hadamard speckle contrast reduction (SCR) technique, which has been successfully used in laser projection systems, in the context of millimeter-wave imaging. We show the impact of Hadamard pattern order and size and of image and pattern resolution on speckle reduction efficiency. Practical limitations of Hadamard pattern implementations and their effect on speckle reduction efficiency are also discussed.     

Recent progress on cooled avalanche photodiodes for single photon detection

Abstract

Recent results on the properties of cooled avalanche photodiodes for single photon detection are presented. Results from Hamamatsu silicon photodiodes, originally developed as radiation-hard photodetectors for high energy physics experiments, are extremely encouraging. Gains of approximately 10,000 can be achieved with the APD operating in proportional mode. Together with a low noise amplifier they allow photon counting with extremely high efficiency and very low noise making cold APDs almost ideal single photon detectors. Operation of APDs in Geiger mode is also reported, together with measurements of detection efficiency and noise as function of operating voltage. Prospects and hopes for future work are briefly summarized.     

Induced Immunity Against Belowground Insect Herbivores- Activation of Defenses in the Absence of a Jasmonate Burst

Roots respond dynamically to belowground herbivore attack. Yet, little is known about the mechanisms and ecological consequences of these responses. Do roots behave the same way as leaves, or do the paradigms derived from aboveground research need to be rewritten? This is the central question that we tackle in this article. To this end, we review the current literature on induced root defenses and present a number of experiments on the interaction between the root herbivore Diabrotica virgifera and its natural host, maize. Currently, the literature provides no clear evidence that plants can recognize root herbivores specifically. In maize, mild mechanical damage is sufficient to trigger a root volatile response comparable to D. virgifera induction. Interestingly, the jasmonate (JA) burst, a highly conserved signaling event following leaf attack, is consistently attenuated in the roots across plant species, from wild tobacco to Arabidopsis. In accordance, we found only a weak JA response in D. virgifera attacked maize roots. Despite this reduction in JA-signaling, roots of many plants start producing a distinct suite of secondary metabolites upon attack and reconfigure their primary metabolism. We, therefore, postulate the existence of additional, unknown signals that govern induced root responses in the absence of a jasmonate burst. Surprisingly, despite the high phenotypic plasticity of plant roots, evidence for herbivore-induced resistance below ground is virtually absent from the literature. We propose that other defensive mechanisms, including resource reallocation and compensatory growth, may be more important to improve plant immunity below ground.