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1.
D Pende R Biassoni C Cantoni S Verdiani M Falco C di Donato L Accame C Bottino A Moretta L Moretta 《Canadian Metallurgical Quarterly》1996,184(2):505-518
Human natural killer (NK) cells express inhibitory receptors that are specific for different groups of HLA-C or HLA-B alleles. The majority of these receptors belong to the immunoglobulin (Ig) superfamily and are characterized by two or three extracellular Ig-like domains. Here we describe a novel inhibitory NK receptor that is specific for a group of HLA-A alleles. The HLA-A3-specific NK cell clone DP7 has been used for mice immunization. Two mAbs, termed Q66 and Q241, bound to the immunizing clone and stained only a subset of NK cell populations or clones. Among Q66 mAb-reactive clones, we further selected those that did not express any of the previously identified HLA-class I-specific NK receptors. These clones did not lyse HLA-A3+ (or -A11+) target cells, but lysis of these targets could be detected in the presence of Q66 or Q241 mAbs. On the other hand, target cells expressing other HLA-A alleles, including -A1, -A2, and -A24, were efficiently lysed. Moreover, none of the HLA-C or HLA-B alleles that were tested exerted a protective effect. Q66+, but not Q66- NK cell clones, expressed messenger RNA coding for a novel 3 Ig domain protein homologous to the HLA-C (p58) and HLA-B (p70) receptors. The corresponding cDNA (cl.1.1) was used to generate transient and stable transfectants in COS7 and NIH3T3 cell lines, respectively. Both types of transfectants were specifically stained by Q66 and Q241 mAbs. Since the cytoplasmic tail of Q66-reactive molecules was at least 11 amino acid longer than the other known p58/p70 molecules, we could generate an antiserum specific for the COOH-terminus of Q66-reactive molecules, termed PGP-3. PGP-3 immunoprecipitated, only from Q66+ NK cells, molecules displaying a molecular mass of 140 kD, under nonreducing conditions, which resolved, under reducing conditions, in a 70-kD band. Thus, differently from the other p58/p70 receptors, Q66-reactive molecules appear to be expressed as disulphide-linked dimers and were thus termed p140. The comparative analysis of the amino acid sequences of p58, p70, and p140 molecules revealed the existence of two cysteins proximal to the transmembrane region, only in the amino acid sequence of p140 molecules. 相似文献
2.
3.
FilippoDiGiovanni GnetanoBazzano AntonioGrimaldi 《今日电子》2003,(3):40-41
功率转换器的功率密度越来越高,发热问题越来越严重,这种功率转换器的设计对现代大功率半导体技术提出了新的挑战;因而,热问题的优化设计和核实变得比大功率器件的电模型更加重要,本文提出一种新的PSPICE模型,可以利用它计算MOSFET芯片在瞬变过程中的温度。其中的热阻可以从制造商提供的产品使用说明书得到。本文介绍的模型提供发热和电气参数之间的动态关系。它建立了与许可的热环境的关系,例如,栅极驱动电路、负载、以及散热器的分析与优化设计。利用这个模型可以改善散热器的设计。由于决定功率损耗的参数是分布在一定范围内,受生产制造的影响很大,因而散热器的设计往往由于无法预先知道功率损耗而无法进行。 相似文献
4.
A Lubics D Regl?di S Slezák M Szelier I Lengvári 《Canadian Metallurgical Quarterly》1997,99(4):459-467
In addition to receptor-type pinealocytes, the mammalian pineal organ contains small and large neurons and ependymal/glial cells as well. Axons of pinealocytes form synaptic ribbon-containing axo-dendritic synapses on large secondary pineal neurons and/or terminate as neurohormonal endings on the basal lamina of the vascular surface of the organ. The small pineal neurons were found to be gamma-aminobutyric acid (GABA)-immunoreactive, while large secondary neurons and pinealocytes contained immunoreactive amino acids (glutamate and aspartate). Glutamate accumulated presynaptically in pinealocytic axon terminals on large secondary neurons and in the axons of these neurons. Glutamate immunoreactive axons of pineal neurons were traced via the pineal tract to the habenular nucleus. Axons containing granular vesicles and coming from extrapineal perikarya are glutamate immunoreactive as well. Aspartate and GABA are also present in some of the myelinated axons, supposedly pinealopetal in the pineal tract. 相似文献
5.
Caterina Dinnella Gaetano Lanzarini Andrea Stagni Claudio Palleschi 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1994,59(3):237-241
Endopectinlyase (EC 4.2.2.10) from Aspergillus japonicus was immobilized on to γ-alumina. Adsorption performed at pH 5·0 and a subsequent cross-linking phase using 0·1% glutaraldehyde were the chosen immobilization conditions. The comparison between the main biochemical parameters of the immobilized and free form of the enzyme showed that the immobilization procedure used did not affect the enzyme biochemical properties. The interactions between the carrier and the enzyme are essentially secondary bonding. In fact they depend on the pH and on the presence of phosphate ions in the medium. A tentative chemical model of the biocatalytic matrix thus obtained is proposed. 相似文献
6.
Kornl L. Kovcs Barna Fodor kos T. Kovcs Gyula Csandi Gergely Marti Judit Balogh Solmaz Arvani Gbor Rkhely 《International Journal of Hydrogen Energy》2002,27(11-12)
The purple (Sulphur) phototrophic bacterium, Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T. roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster hoxEFUYH was identified and sequenced. In addition, genes homologous to hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to hypF, the following accessory genes were identified: hydD, hupK, hypC1, hypC2, hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress. 相似文献
7.
Homer M.E. Hogan S.J. di Bernardo M. Williams C. 《Circuits and Systems II: Express Briefs, IEEE Transactions on》2004,51(10):511-516
In this brief, we consider methods to improve the performance of chaotic communication schemes. We study a system using a receiver which explicitly includes the presence of noise in the channel. We show how the choice of chaotic dynamical system generating the transmitted signal is crucial. We observe a large variation in bit error rate performance of the system as parameters in the maps are changed, and we propose a simple explanation for this variation. 相似文献
8.
Leonardo Setti Pier Giorgio Pifferi Gaetano Lanzarini 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1995,64(1):41-48
A new mathematical model for n-alkane biodegradation in crude oil, heavy oil and paraffinic mixtures is described. The pattern of n-alkane degradation as a function of the inverse of hydrocarbon chain length reported in this paper can be considered as general behaviour for many aerobic n-alkane biodegradation processes. A new interpretation of n-alkane biodegradation as a function of surface tension, is given. A mathematical expression was obtained starting from the degradation values of n-alkane and relative surface tension, which is a parameter independent of fermentation conditions. An interesting parameter, b, was identified which represented the accelerating conversion factor for n-alkane biodegradation. The findings suggested that the n-alkane biodegradation. The findings suggested that he n-alkane biodegradation rate may be affected by the fermentation condition (agitation, aeration, etc.) and by the strain of microorganism, while the behaviour pattern of n-alkane degradation was essentially linked to the substrate characteristics (molecular structure, molecular weight and density). 相似文献
9.
A. Di Chiara U. Scotti di Uccio M. Senatore L. Maritato 《Journal of Low Temperature Physics》1986,62(5-6):385-396
Results concerning V3Si films produced by a simple annealed multilayer technique are reported together with X-ray diffraction patterns, Auger spectroscopy, and Rutherford backscattering analysis. Low-temperature electrical resistivity measurements are discussed. It is found that the V3Si films exhibit aT
2 dependence in the temperature rangeT
c
T23 K and aT
2.6 dependence in the rangeT
c
T40 K. The normal-state resistivity in the whole temperature range (T
c
T600 K) is analyzed in the framework of Cote-Meisel theory. Consistent values of the saturation resistivity
m
and of the Debye temperature are obtained by fitting the experimental data with the Cote-Meisel expression for (T). 相似文献
10.
Modeling guided wave propagation with application to the long-range defect detection in railroad tracks 总被引:2,自引:0,他引:2
Ivan Bartoli Francesco Lanza di Scalea Mahmood Fateh Erasmo Viola 《NDT & E International》2005,38(5):325-334
The aim of this work is to demonstrate the use of a commercial finite element package, ABAQUS EXPLICIT, to model ultrasonic guided waves in structural components. The particular application of interest is modeling the interaction of a broadband vertical bending mode with transverse-type defects in railroad tracks. This topic is part of a broader project on high-speed defect detection in rails by long-range ultrasonic inspections. Reflection coefficient spectra in the 20–45 kHz range are obtained for four different sizes and three different orientations of transverse head flaws. A preliminary study of Lamb waves in a free plate helps drawing modeling guidelines for the rail. 相似文献