Methods to assess physical activity with special reference tomotion sensors and accelerometers

Motion sensors may be applied for the assessment of physical activity. This paper reviews the evolution of these instruments from the mechanical pedometer to the electronic accelerometer. We conclude that for accurate assessment of physical activity under free living conditions the recently introduced accelerometer looks most promising, although little information was available regarding the reliability of these instruments. Subsequently, reliability of an accelerometer with a three-directional sensor was examined. Intrainstrument variation in a bench test was less than 8% during four measurements over a week. Interinstrument variation during treadmill experiments while subjects wore two accelerometers at the same time was on average 22% and was not improved after adjustment for differences found in the bench test. Reproducibility in the treadmill experiment was approximately 76, 85, and 95% at 3, 5, and 7 km/h, respectively. Bench testing revealed that the sensitivity of a piezoelectric element is prone to shifts, probably due to mechanical, electromagnetic, and/or temperature shock, which may be encountered during outdoor application. However, the relevance of the bench test in this study may be questioned, as results did not correspond with the findings in subjects. This needs further investigation.     

Linoleic acid hydroperoxide concentration in relation to mutagenicity of repeatedly used deep-frying fats

A high-performance liquid chromatography procedure was developed to measure linoleic acid hydroperoxides (LAHPO) in 19 used, deep-frying fat samples that were screened for mutagenicity. The detection limit of the method was found to be 10 nmol of LAHPO/g of fat. In eight samples, LAHPO were not detected; levels in the other samples ranged from 17 to 267 nmol/g of fat. LAHPO were not detectable in unusued hydrogenated frying fat samples. Concentrations of LAHPO correlated positively with mutagenicity to Salmonella tester strains TA97 and TA100, in presence of S9 mix only; coefficients of correlation were, respectively, r=0.48 (p<0.05) and r=0.24 (n.s.). Without metabolic activation no significant associations were observed. These results suggest that metabolites or secondary autoxidation products of linoleic acid generated in presence of liver S9 mix may contribute to mutagenicity of some of the used deep-frying fat samples.     

Auditory spatial alternation transforms auditory time (again): comments on Lakatos (1993), "Temporal constraints on apparent motion in auditory space"

Lakatos (1993) reported interesting data that indeed support "the hypothesis that the extent of spatial separation between successive sound events directly affects the perception of time intervals between these events" (p. 139). The present comment is an attempt to show that, as far as the horizontal plane is concerned, Lakatos's hypothesis was already answered qualitatively by Axelrod and coworkers (Axelrod & Guzy, 1968; Axelrod, Guzy, & Diamond, 1968) in their studies of attention shifting, and by ten Hoopen and coworkers, who quantified the amount of "interaural time dilation" (Akerboom, ten Hoopen, Olierook, & van der Schaaf, 1983; ten Hoopen, 1982; ten Hoopen, Vos, & Dispa, 1982). Nonetheless, Lakatos's study is very worthwhile. It originated from the realm of "apparent motion paradigms," but I will argue that the study rather used an "auditory streaming paradigm," and that the data is a welcome contribution to elucidate how the perceptual processes of auditory stream formation and interaural time dilation interact.     

Spatial frequencies in aesthetic website evaluations--explaining how ultra-rapid evaluations are formed

This study investigates how aesthetic website evaluations, especially those formed after very brief presentations, depend on visual information that is encoded in low- or high-spatial frequencies. A total of 92 participants took part in the experiment. The study used a 3 × 3 mixed design in which presentation time (50, 500 and 10000 ms) and spatial filtering (low-pass filtered, high-pass filtered and unfiltered stimuli) were manipulated. First, we replicate prior results from online studies of high- and low-spatial frequencies. Second, we confirm a prediction from neurocognitive models that only low-spatial frequencies are relevant to aesthetic judgements in ultra-rapid presentation modes. Third, we demonstrate that stimulus repetitions lead to an overestimation of the importance of ultra-rapid stimulus presentations. Taken together, our results highlight the utility of neurocognitive models of visual processing to explain the rapid aesthetic evaluation of websites. PRACTITIONER SUMMARY: Using neurocognitive models we present an approach to explain how aesthetic impressions are formed. We show that ultra-rapid judgements are connected with low- but not with high-spatial frequencies, which are neurologically processed in different visual pathways. Furthermore we identify possible methodological problems in previous studies of ultra-rapid website perception.     

Monadic second-order definable text languages

Atext is a word together with a (additional) linear ordering. Each text has a generic tree representation, called itsshape. Texts are considered in a logical and in an algebraic framework. It is proved that, for texts of bounded primitivity, the class of monadic second-order definable text languages coincides with both the class of recognizable text languages and the class of text languages generated by right-linear text grammars. In particular it is demonstrated that the construction of the shape of a text can be formalized in terms of our monadic second-order logic. This approach can be extended to the case of graphs. This research was supported by the EBRA Working Group ASMICS 2.     

Microscopy under pressure--an optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure

High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To directly monitor "cells under pressure," the development of an optical high-pressure chamber is required. Therefore, an optical pressure chamber that can be used for up to 300 MPa was constructed. This chamber has already been described as a tool for in situ observation of dynamic changes of microscopic structures in bright field as well as phase contrast. In combination with an inverted microscope, we obtained brilliant microscopic color pictures with an optical resolution more than 0.56 microm. Here, we demonstrate the capabilities of the HHP cell, in combination with epifluorescence microscopy. Using a nonadherent human B-cell line (Raji, ATCC CCL 86), stained with the fluorescent dyes propidium iodide, Hoechst 33342, or dihexyloxacarbocyanine iodide, we were able to show that the system is suitable to perform fluorescence microscopic analyses, with pressures up to 300 MPa, with viable mammalian cells.     

Systematic Review: Targeted Molecular Imaging of Angiogenesis and Its Mediators in Rheumatoid Arthritis

Extensive angiogenesis is a characteristic feature in the synovial tissue of rheumatoid arthritis (RA) from a very early stage of the disease onward and constitutes a crucial event for the development of the proliferative synovium. This process is markedly intensified in patients with prolonged disease duration, high disease activity, disease severity, and significant inflammatory cell infiltration. Angiogenesis is therefore an interesting target for the development of new therapeutic approaches as well as disease monitoring strategies in RA. To this end, nuclear imaging modalities represent valuable non-invasive tools that can selectively target molecular markers of angiogenesis and accurately and quantitatively track molecular changes in multiple joints simultaneously. This systematic review summarizes the imaging markers used for single photon emission computed tomography (SPECT) and/or positron emission tomography (PET) approaches, targeting pathways and mediators involved in synovial neo-angiogenesis in RA.     

Iterative demapping for QPSK modulation

The authors present an iterative decoding scheme which is particularly suited for quadrature phase shift keying (QPSK) modulation with anti-Gray mapping. At the receiver, a soft demapper accepting a priori information and a convolutional decoder are applied in a serial concatenation. Numerical results are presented for QPSK in additive white Gaussian noise and Rayleigh-fading channels. The iterated system applying anti-Gray mapping outperforms the conventional system using Gray mapping after only one iteration     

Convergence behavior of iteratively decoded parallel concatenatedcodes

Mutual information transfer characteristics of soft in/soft out decoders are proposed as a tool to better understand the convergence behavior of iterative decoding schemes. The exchange of extrinsic information is visualized as a decoding trajectory in the extrinsic information transfer chart (EXIT chart). This allows the prediction of turbo cliff position and bit error rate after an arbitrary number of iterations. The influence of code memory, code polynomials as well as different constituent codes on the convergence behavior is studied for parallel concatenated codes. A code search based on the EXIT chart technique has been performed yielding new recursive systematic convolutional constituent codes exhibiting turbo cliffs at lower signal-to-noise ratios than attainable by previously known constituent codes     

Contribution of the C-terminal amino acid to the stability of Bacillus subtilis neutral protease

The role of the C-terminal Leu300 in maintaining thermal stabilityof the neutral protease of Bacillus subtilis was investigated.From model building studies based on the three dimensional structureof thermolysin, the neutral protease of B.thermoproteolyticus,it was conduded that this residue is located in a hydrophobicpocket composed of residues located in the C-terminal and themiddle domain. To test the hypothesis that Leu300, by contributingto a stabilizing interaction between these domains, is importantfor enzyme stability, several neutral protease mutants wereconstructed and characterized. The thermostability of the enzymewas lowered by deleting Leu300 or by replacing this residueby a smaller (Ala), a polar (Asn) or a sterically unfavourable(He) amino acid. Thermostabiity was increased upon replacingLeu300 by Phe. These results are in agreement with model-buildingstudies. The effects on thermostability observed after mutatingthe corresponding Val318 in the thermostable neutral proteaseof B.stearothermophilus were less pronounced.