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AO Johnson A Subtil R Petrush K Kobylarz SR Keller TE McGraw 《Canadian Metallurgical Quarterly》1998,273(28):17968-17977
In adipocytes, the insulin-regulated aminopeptidase (IRAP) is trafficked through the same insulin-regulated recycling pathway as the GLUT4 glucose transporter. We find that a chimera, containing the cytoplasmic domain of IRAP fused to transmembrane and extracellular domains of the transferrin receptor, is slowly recycled and rapidly internalized in Chinese hamster ovary cells. Morphological studies indicate that the chimera is slowly trafficked through the general endosomal recycling compartment rather than being sorted to a specialized recycling pathway. A chimera in which a di-leucine sequence within the cytoplasmic domain of IRAP has been mutated to alanines is rapidly internalized and rapidly recycled, indicating that this di-leucine is required for the slow recycling but not for the rapid internalization. Insulin stimulates a 2-3-fold increase in the recycling of the chimera and only a 1.2-fold increase in the recycling of the transferrin receptor. The effect of insulin on the recycling of the chimera is blocked by wortmannin, a phosphatidylinositol 3'-kinase inhibitor. GTPgammaS (guanosine 5'-3-O-(thio)triphosphate) increases the recycling of the chimera by 50% but has no effect on the recycling of the transferrin receptor. In these studies, we have identified in Chinese hamster ovary cells a novel, slow endocytic recycling mechanism that is regulated by insulin. 相似文献
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An automatically tuning intrusion detection system. 总被引:3,自引:0,他引:3
Zhenwei Yu Jeffrey J P Tsai Thomas Weigert 《IEEE transactions on systems, man, and cybernetics. Part B, Cybernetics》2007,37(2):373-384
An intrusion detection system (IDS) is a security layer used to detect ongoing intrusive activities in information systems. Traditionally, intrusion detection relies on extensive knowledge of security experts, in particular, on their familiarity with the computer system to be protected. To reduce this dependence, various data-mining and machine learning techniques have been deployed for intrusion detection. An IDS is usually working in a dynamically changing environment, which forces continuous tuning of the intrusion detection model, in order to maintain sufficient performance. The manual tuning process required by current systems depends on the system operators in working out the tuning solution and in integrating it into the detection model. In this paper, an automatically tuning IDS (ATIDS) is presented. The proposed system will automatically tune the detection model on-the-fly according to the feedback provided by the system operator when false predictions are encountered. The system is evaluated using the KDDCup'99 intrusion detection dataset. Experimental results show that the system achieves up to 35% improvement in terms of misclassification cost when compared with a system lacking the tuning feature. If only 10% false predictions are used to tune the model, the system still achieves about 30% improvement. Moreover, when tuning is not delayed too long, the system can achieve about 20% improvement, with only 1.3% of the false predictions used to tune the model. The results of the experiments show that a practical system can be built based on ATIDS: system operators can focus on verification of predictions with low confidence, as only those predictions determined to be false will be used to tune the detection model. 相似文献
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A Combined Surface Science and Electrochemical Study of Tungsten Carbides as Anode Electrocatalysts 总被引:1,自引:0,他引:1
Erich C. Weigert Michael B. Zellner Alan L. Stottlemyer Jingguang G. Chen 《Topics in Catalysis》2007,46(3-4):349-357
An effective anode electrocatalyst in direct methanol fuel cell (DMFC) should have high activity for the oxidation of methanol
and the decomposition of water, while remaining stable under the relatively harsh anode environment. Although the Pt/Ru bimetallic
alloy is currently the most effective anode electrocatalyst, both Pt and Ru are expensive due to limited supplies and both
are susceptible to CO poisoning. Consequently, the discovery of less expensive and more CO tolerant alternatives to the Pt/Ru
catalysts would help facilitate the commercialization of DMFC. In this paper we will discuss the possibility of using tungsten
carbides (WC) and Pt-modified WC as potential anode electrocatalysts in DMFC. We will provide an overview of our recent work,
using a combined approach of fundamental surface science studies and in-situ electrochemical evaluation of the activity and
stability of tungsten carbides. We will demonstrate the feasibility to bridge fundamental surface science studies on single
crystals with the electrochemical evaluation on polycrystalline WC films. We will also discuss the synergistic effect by supporting
low coverages of Pt on the WC substrate to further enhance the electrochemical performance of WC. 相似文献
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PURPOSE: Prosthetic heart valve thrombosis occurring during pregnancy is a life-threatening complication. Surgical treatment requires clot removal under cardiopulmonary bypass (CPB) and carries a high mortality. We describe the successful use of thrombolytic therapy for recurrent thrombosed valve prosthesis in a pregnant patient. CLINICAL FEATURES: A 32-yr-old patient whose pregnancy was complicated by two episodes of a thrombosed St Jude mitral prosthesis is reported. The first episode occurred at 20 wk of pregnancy during the change of oral anticoagulant therapy (acenocoumarol 4 mg a day) to sc heparin. As the patient was in cardiogenic shock, the valve thrombus was treated by clot removal under CPB., with a cross clamp time of 32 min, a perfusion pressure above 70 mmHG. There was no fetal cardiac rhythm during CPB which lasted < 45 min. The second episode occurred at the 28th gestational week in a patient in cardiogenic shock and because reoperation was thought to carry too high a risk, the thrombus was successfully treated with 50 mg recombinant tissue plasminogen activators (rtPA) i.v. Following this, the course of pregnancy was uneventful and carried to term and the patient delivered vaginally. Pain relief was achieved with intravenous patient-controlled analgesia with alfentanil (bolus 100 mug; lock out = five minutes). Although rtPA has been used before, this is the first report in which pregnancy was carried to term and standard vaginal delivery performed. CONCLUSION: This case provides evidence for the efficacy and relative safety of rtPA as thrombolytic therapy in the treatment of haemodynamically compromised valve heart thrombosis in pregnancy. 相似文献
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There have been only few attempts to extend fuzzy logic to automated theorem proving. In particular, the applicability of the resolution principle to fuzzy logic has been little examined. The approaches that have been suggested in the literature, however, have made some semantic assumptions which resulted in limitations and inflexibilities of the inference mechanism. In this paper we present a new approach to fuzzy logic and reasoning under uncertainty using the resolution principle based on a new operator, the fuzzy operator. We present the fuzzy resolution principle for this logic and show its completeness as an inference rule. 相似文献
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