Electrospun curcumin/polycaprolactone/copolymer F-108 fibers as a new therapy for wound healing

The application of fibers associated with drugs is a promising alternative to meet the clinical needs of tissue repair. Curcumin exhibits great cicatricial potential because it has numerous pharmacological properties. This research aimed to produce fibers of polycaprolactone and copolymer F-108 associated with curcumin and to evaluate in vivo their action on the process of wound healing. The fibers were produced by electrospinning technique and characterized by scanning electron microscopy (SEM), X-ray diffractometry (XRD), and fluorescence microscopy. They were applied in cutaneous wounds of rats for the analysis of photoacoustic permeation and histological study. The characterization showed that the electrospinning allowed the preparation of homogeneous material with curcumin. The fibers benefited healing of the wounds and allowed the permeation of curcumin at all stages. The use of PCL/F-108 fibers allowed the elaboration of a new curcumin delivery system, improving its bioavailability and action in the healing of excisional wound. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48415.     

On a mechanism for isochromatid 17q in a subset of Ph+ chronic myeloid leukemia patients

Considerable advances have been made in reconstructive venous surgery in the past two decades because of an increased recognition of venous disease and because of extensive experimental work. Meaningful evaluation of longterm patency of bypass grafts and midterm patency of stent placement are now available and seem appropriate to provide guidelines. Based on our experience and on the literature, this article highlights the techniques and the respective indications of surgical and endovascular treatment of large vein reconstructions.     

Variational (Lagrangian) approach to myeloid differentiation path(s)

This study was designed to determine the bioavailability of etoposide capsules administered orally at doses of 50 and 75 mg. Patients with inoperable or relapsed lung cancer, who had an Eastern Cooperative Oncology Group (ECOG) performance status of 0-2 and adequate organ function, were eligible. A group of 17 patients were evaluable, all of whom were 75 years old or less, with an ECOG performance status of 0 or 1. The bioavailability of oral etoposide was determined by measuring the area under the etoposide plasma concentration versus time curve (AUC) on days 1, 10 and 21 during a once-daily regimen of oral administration for 21 consecutive days and comparing the value with the AUC achieved following intravenous administration 1 or 2 weeks after the last oral dose. The bioavailability of 50, 75 and 100 mg oral etoposide was determined in six, nine and two patients, respectively. The mean etoposide bioavailabilities (+/- SD) of the 50-mg and 75-mg doses were 47 +/- 11% and 59 +/- 18%, respectively, and of the 100-mg dose in two patients were 51% and 33%, respectively. There was no statistically significant difference in bioavailability between the 50-mg and 75-mg doses. The bioavailability of low-dose oral etoposide was the same as that reported in previous higher dose oral etoposide bioavailability studies and that shown on the package insert supplied by the manufacturer. Improved bioavailability of low-dose oral etoposide was therefore not observed in a population of Japanese patients.     

Improved Production of Cyclodextrins by Alkalophilic Bacilli Immobilized on Synthetic or Loofa Sponges

This study aimed to improve the production of β-cyclodextrin (β-CD) by microbial cells immobilized on synthetic or loofa sponges both with and without the use of alginate or chitosan. The most suitable matrix for the immobilization of Bacillus firmus strain 7B was synthetic sponge and for Bacillus sphaericus strain 41 was loofa sponge. After 330 days of storage, the β-CD production by Bacillus firmus and Bacillus sphaericus remained at around 41% and 49%, respectively, of initial levels. After 24 days of immobilization on loofa sponge, Bacillus sphaericus strain 41 achieved an improved operational stability, reaching 86.6 mM β-CD after 20 days of production, compared to only 32.8 mM of β-CD produced by free Bacillus sphaericus strain 41 cells. The expected increase in β-CD production by immobilized cells of Bacillus firmus strain 7B on synthetic sponge for 4 days was not statistically different to that for cells immobilized for 24 days. The application of this process on an industrial scale using loofa sponge, an inexpensive and renewable matrix, will allow the stable production of β-CD.     

Method development and validation for analysis of microencapsulated cyanidin-3-O-rutinoside in dairy samples containing juçara palm fruit by high-performance liquid chromatography

Euterpe edulis is a plant native of Brazil and popularly known as juçara. The aim of this study was to develop and validate an analytical method using high-performance liquid chromatography (HPLC) of juçara crude extracts (JCEs) and to evaluate dairy products containing free and microencapsulated extracts. The validated analytical method for JCE was performed using cyanidin-3-O-rutinoside (C3R – isolated and identified as major component from JCE) as a reference: linearity was obtained using ten concentrations between 25 and 250 μg mL⁻¹ (R² = 0.994); limits of detection and quantification were 19.04 μg mL⁻¹ and 57.7 μg mL⁻¹ respectively; accuracy (relative standard deviation) was <5%; recovery was 104.85 μg mL⁻¹; and the method is robust. Physicochemical analyses of the dairy products were evaluated using colour, pH, and concentration of anthocyanins using HPLC calculated as C3R over 21 days of storage. The pH remained stable until the end of the test. The colour and the concentration of anthocyanins remained stable for 9 days. C3R was isolated, the HPLC method was developed and validated, and the colour stability was not altered until 9 days. © 2023 Society of Chemical Industry.     

Butter cholesterol removal using different complexation methods with beta-cyclodextrin,and the contribution of photoacoustic spectroscopy to the evaluation of the complex

The aim of this study was to evaluate the removal of butter cholesterol through three complexation methods with beta-cyclodextrin (beta-CD): co-precipitation, kneading, and physical mixture. Photoacoustic spectroscopy (PAS) was used to evaluate the inclusion complex cholesterol in beta-CD. The co-precipitation method was the most appropriate for removal of butter cholesterol. The composition in fatty acids was not affected by the adopted process. The standardization of the extraction technique of butter cholesterol for its quantification was efficient. The comparison between three quantification methods, enzymatic, high-performance liquid chromatography, and gas chromatography, showed no significant differences. PAS allowed to evaluate the extraction of butter cholesterol, and showed that the amount of beta-CD used can be reduced. The results showed that the use of this technique opens new prospects to confirm the formation of the inclusion complex, the “guest-beta-CD”.     

Molecular adaptation of Drosophila melanogaster lysozymes to a digestive function

A lysozyme (pI 5.5) was purified to homogeneity from heated acid extracts of Drosophila melanogaster larvae, using gel filtration in a Superose column and ion-exchange chromatography in a Mono Q column. The final yield was 67%. The purified lysozyme with Mr 13,700 (determined by SDS-polyacrylamide gel electrophoresis) decreases in activity and has its pH optimum displaced towards acidic values and Km increases as the ionic strength of the medium becomes higher. The lysozyme is resistant to a cathepsin D-like proteinase present in cyclorrhaphous Diptera and displays a chitinase activity which is 11-fold higher than that of chicken lysozyme. Microsequencing of an internal peptide of the purified lysozyme showed that this enzyme is the product of the previously sequenced Lys D gene. The results suggest that the product of the Lys P gene has pI 7.2, a pH optimum around 5 and is not a true digestive enzyme. The most remarkable sequence convergence of D. melanogaster lysozyme D and lysozymes from vertebrate foregut fermenters are serine 104 and a decrease in the number of basic amino acids, suggesting that these features are necessary for digestive function in an acid environment. Adaptive residues putatively conferring stability in an acid proteolytic environment differ between insects and vertebrates, probably because they depend on the overall three-dimensional structure of the lysozymes. A maximum likelihood phylogeny and inferences from insect lysozyme sequences showed that the recruitment of lysozymes as digestive enzymes is an ancestral condition of the flies (Diptera: Cyclorrhapha).