Magnetic Resonance Signal Amplification by Reversible Exchange of Selective PyFALGEA Oligopeptide Ligands Towards Epidermal Growth Factor Receptors

The biorelevant PyFALGEA oligopeptide ligand, which is selective towards the epidermal growth factor receptor (EGFR), has been successfully employed as a substrate in magnetic resonance signal amplification by reversible exchange (SABRE) experiments. It is demonstrated that PyFALGEA and the iridium catalyst IMes form a PyFALGEA:IMes molecular complex. The interaction between PyFALGEA:IMes and H₂ results in a ternary SABRE complex. Selective 1D EXSY experiments reveal that this complex is labile, which is an essential condition for successful hyperpolarization by SABRE. Polarization transfer from parahydrogen to PyFALGEA is observed leading to significant enhancement of the ¹H NMR signals of PyFALGEA. Different iridium catalysts and peptides are inspected to discuss the influence of their molecular structures on the efficiency of hyperpolarization. It is observed that PyFALGEA oligopeptide hyperpolarization is more efficient when an iridium catalyst with a sterically less demanding NHC ligand system such as IMesBn is employed. Experiments with shorter analogues of PyFALGEA, that is, PyLGEA and PyEA, show that the bulky phenylalanine from the PyFALGEA oligopeptide causes steric hindrance in the SABRE complex, which hampers hyperpolarization with IMes. Finally, a single-scan ¹H NMR SABRE experiment of PyFALGEA with IMesBn revealed a unique pattern of NMR lines in the hydride region, which can be treated as a fingerprint of this important oligopeptide.     

The influence of volume fraction of amorphous phase on corrosion resistance of Mg67Zn 29Ca 4 alloy

The aim of this study was to investigate the structure and corrosion resistance of amorphous, amorphous‐crystalline, and crystalline Mg₆₇Zn₂₉Ca₄ alloy for biodegradable applications. This paper presents a preparation method and results of the structural characterization and corrosion resistance analysis of the material. Samples were prepared in the form of 3 mm diameter rods. The structure of the alloy was examined with the use of X‐ray diffractometry and scanning electron microscopy. The thermal properties of the samples were examined with differential scanning calorimetry (DSC). Results of DSC analysis were used to determine heat treatment temperatures, allowing to obtain different fractures of crystalline phase in the material. Corrosion resistance of heat‐treated samples was investigated by immersion tests and electrochemical measurements performed in the simulated body fluid. The X‐ray diffraction results confirmed that the prepared Mg₆₇Zn₂₉Ca₄ alloy's structure is fully amorphous. After heat treatment, samples with different fractions of amorphous phase in the structure were obtained. Immersion tests of the samples showed that the structure significantly influenced corrosion resistance in examined materials. It should be pointed out, that certain amounts of crystalline phase in amorphous matrix can greatly improve the corrosion resistance of Mg₆₇Zn₂₉Ca₄ alloy.     

Anodising potential influence on well-ordered nanostructures formed by anodisation of aluminium in sulphuric acid

High-density structures with relatively well-ordered nanopore arrays have been obtained by the self-ordering growth of nanopores occurring during anodisation of aluminium in sulphuric acid. The resulting array of well-ordered nanopores strongly depends on an applied voltage of anodising, temperature and a procedure of synthesis. Regular arranged hexagonal arrays on aluminium with a relatively uniform pore diameter, interpore distance, and depth of pores exceeding 90 μm, can be formed by the self-ordering two-step anodising at 1 °C. The interpore distance and the pore diameter were evaluated on anodised aluminium layers obtained at different cell potentials ranging from 15 to 25 V. The detailed analyses of their uniformity were performed from SEM images. The analysis of a pore arrangement defects was made from SEM top view images taken on samples anodised at various cell potentials. The defect maps, known as Delaunay triangulations, of the arrangement of about 1000 pores were constructed for different applied anodising potentials. The percentage of pores that are not six-fold coordinated by the neighbouring pores indicates the percentage of defected surface.     

The Construction of Minimal DNA Expressions

We describe a formal language/notation for DNA molecules that may contain nicks and gaps. The elements of the language, DNA expressions, denote formal DNA molecules. Different DNA expressions may denote the same formal DNA molecule. We analyse the shortest DNA expressions denoting a given formal DNA molecule. We determine lower bounds on their lengths and explain how we construct these minimal DNA expressions.     

Effects of uncontrollability on subsequent decision making: Testing the cognitive exhaustion hypothesis.

The cognitive exhaustion model of helplessness (predicting withdrawal from constructive effortful processing after uncontrollability) was applied to decision making. After unsolvable problems (or no preexposure), Ss requested information from a matrix with 5 alternatives (films)?×?10 attributes and then chose the best film. Films in a set were either similar (difficult decision) or dissimilar (easy decision) in attractiveness. As predicted, Ss with an uncontrollable preexposure spent less time on predecisional information search, disregarded their own importance criteria when asking for information, and had attention highly focused on a selected option for the easy decision condition but diffused across options for the difficult decision condition. The implications of these findings for understanding cognitive mechanisms of learned helplessness and depression are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Microstructural transformations of heat affected zones in duplex steel welded joints

The influence of the welding thermal conditions exemplified by heat input and heat treatment after welding on the structure of the heat affected zone (HAZ) UNS S31803 has been analysed. The post weld treatment was used to create the precisely defined thermal conditions for the decomposition of primary phases in the HAZ, by a multi-layer welding thermal cycle stimulation. Detailed analyses of the microstructure and chemical composition of the phases in the different post welded conditions were performed by scanning electron microscopy (SEM) combined with energy dispersive spectrometry (EDS) and transmission electron microscopy (TEM). Three types of secondary precipitates have been observed: secondary austenite (γ₂), carbides: M₂₃C₆ and M₇C₃. The dependence of the secondary austenite volume fraction and morphology in the HAZ on thermal cycle have been interpreted. The eutectoid decomposition of the primary phases in the analysed thermal conditions was confirmed.     

Embedding linear orders in grids

A grid (or a mesh) is a two-dimensional permutation: an m× n-grid of size mn is an m× n-matrix where the entries run through the elements {1,2, …, mn}. We prove that if δ₁ and δ₂ are any two linear orders on {1,2, …, N}, then they can be simultaneously embedded (in a well defined sense) into a unique grid having the smallest size.