Flavonoids of “La Alcarria” honey A study of their botanical origin

Summary The flavonoids present in ten selected samples of La Alcarria honey with different pollen compositions have been HPLC analysed in order to establish if correlations between botanical origin and flavonoid profiles are possible. A common flavonoid pattern is observed in the different samples showing that pollen is not the main source of honey flavonoids. A close correlation between the flavonoid patterns of honey flavonoids and propolis flavonoids has been found suggesting that flavonoid analysis could be more useful in geographical origin determinations than in botanical origin studies.

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Flavonoide des La-Alcarria-Honigs Eine Studie ihres botanischen Ursprungs

Zusammenfassung Die Flavonoide in Proben in Alcarria-Honig mit unterschiedlicher Pollenzusammensetzung wurden untersucht, um Kortrelationen zwischen dem botanischen Ursprung und den möglichen Flavonoiden zu finden. In den verschiedenen Proben wurde ein Flavonoid-Muster gefunden, wobei der Pollen nicht die Hauptquelle der Honigflavonoide ist. Es wurde jedoch eine enge Korrelation zwischen den Flavonoid-Mustern des Honigs und des Bienenkittharzes gefunden, was für die geographische Herkunft wichtiger ist als die botanische.

     

Floral nectar phenolics as biochemical markers for the botanical origin of heather honey

In order to find out biochemical markers for the botanical origin of heather (Erica) honey, the phenolic metabolites present in heather floral nectar, collected from the honey-stomach of bees gathering nectar from these flowers, were analysed. The flavonoid fraction of nectar contained four main flavonoids. Two of them were quercetin and kaempferol 3-rhamnosides, and the other two were tentatively identified as myricetin 3-methyl ether and isorhamnetin 3-rhamnosides. Since the natural glycosides are hydrolysed by bee enzymes to render the corresponding aglycones, which are the metabolites detected in honey, acid hydrolysis of the nectar glycosides was achieved. The aglycones quercetin, myricetin 3-methyl ether, kaempferol and isorhamnetin were identified, as well as the gallic acid derivative ellagic acid. The analysis of Portuguese heather honey samples showed that ellagic acid was present in all the samples in significant amounts ranging between 100 g and 600 g per 100 g honey. The other nectar-derived flavonoids were also present, although some of them in very variable amounts. Ellagic acid and myricetin 3-methyl ether, which have not been detected in any of the monofloral honey samples investigated so far, with the only exception being a French honey sample of the botanically relatedCalluna (Ericaceae) which also contained ellagic acid, seem to be the most useful potential markers for the floral origin of heather honey. However, more detailed and extensive investigations are needed to prove the utility of these markers.     

Incremental Learning of Skills in a Task-Parameterized Gaussian Mixture Model

Programming by demonstration techniques facilitate the programming of robots. Some of them allow the generalization of tasks through parameters, although they require new training when trajectories different from the ones used to estimate the model need to be added. One of the ways to re-train a robot is by incremental learning, which supplies additional information of the task and does not require teaching the whole task again. The present study proposes three techniques to add trajectories to a previously estimated task-parameterized Gaussian mixture model. The first technique estimates a new model by accumulating the new trajectory and the set of trajectories generated using the previous model. The second technique permits adding to the parameters of the existent model those obtained for the new trajectories. The third one updates the model parameters by running a modified version of the Expectation-Maximization algorithm, with the information of the new trajectories. The techniques were evaluated in a simulated task and a real one, and they showed better performance than that of the existent model.     

Facile Synthesis of Amine-Functionalized Eu3+-Doped La(OH)3 Nanophosphors for Bioimaging

Here, we report a straightforward synthesis process to produce colloidal Eu³⁺-activated nanophosphors (NPs) for use as bioimaging probes. In this procedure, poly(ethylene glycol) serves as a high-boiling point solvent allowing for nanoscale particle formation as well as a convenient medium for solvent exchange and subsequent surface modification. The La(OH)₃:Eu³⁺ NPs produced by this process were ~3.5 nm in diameter as determined by transmission electron microscopy. The NP surface was coated with aminopropyltriethoxysilane to provide chemical functionality for attachment of biological ligands, improve chemical stability and prevent surface quenching of luminescent centers. Photoluminescence spectroscopy of the NPs displayed emission peaks at 597 and 615 nm (λ_ex = 280 nm). The red emission, due to ⁵D₀ → ⁷F₁ and ⁵D₀ → ⁷F₂ transitions, was linear with concentration as observed by imaging with a conventional bioimaging system. To demonstrate the feasibility of these NPs to serve as optical probes in biological applications, an in vitro experiment was performed with HeLa cells. NP emission was observed in the cells by fluorescence microscopy. In addition, the NPs displayed no cytotoxicity over the course of a 48-h MTT cell viability assay. These results suggest that La(OH)₃:Eu³⁺ NPs possess the potential to serve as a luminescent bioimaging probe.     

Application of real/mixed μ computational techniques to an H∞ missile autopilot

A multivariable missile autopilot is synthesized using an H_∞ approach. A tradeoff is achieved between performance, actuators solicitation and uncertainties in the actuators and bending modes dynamics. Robust stability and performance of the control law are then studied in the face of large real parametric aerodynamic uncertainties: computational techniques for real and mixed μ analysis (namely De Gaston and Safonov's, Dailey's, Jones’, Young and Doyle's, Fan, Tits and Doyle's and Safonov and Lee's methods) are briefly reviewed before being used to compute either the exact value, or an interval of the structured singular value (SSV). For small amounts of parameters, the upper and lower bounds provided by these methods are compared to the exact value, computed by De Gaston and Safonov's method. For larger amounts of parameters, NP hardness of the problem prohibits the use of algorithms which compute the exact value: these algorithms are indeed necessarily exponential-time. As an alternative in this case, the use of polynomial-time methods for computing upper and lower bounds leads in our examples to accurate approximates of the real and mixed structured singular values.     

Undecidable properties of flat term rewrite systems

Reachability, joinability and confluence properties are known to be undecidable for flat term rewrite systems (TRS). We give shorter and conceptually simpler proofs of these results. We also prove undecidability of weak normalization and unique normalization properties for flat TRS. The first author was supported by Spanish Ministry of Education and Science by the FORMALISM project (TIN2007-66523) and by the LOGICTOOLS-2 project (TIN2007-68093-C02-01). The second author was supported by Spanish Ministry of Education and Science by the FORMALISM project (TIN2007-66523).     

The role of K+ and H+ transport systems during glucose‐ and H2O2‐induced cell death in Saccharomyces cerevisiae

Glucose, in the absence of additional nutrients, induces programmed cell death in yeast. This phenomenon is independent of yeast metacaspase (Mca1/Yca1) and of calcineurin, requires ROS production and it is concomitant with loss of cellular K⁺ and vacuolar collapse. K⁺ is a key nutrient protecting the cells and this effect depends on the Trk1 uptake system and is associated with reduced ROS production. Mutants with decreased activity of plasma membrane H⁺‐ATPase are more tolerant to glucose‐induced cell death and exhibit less ROS production. A triple mutant ena1‐4 tok1 nha1, devoid of K⁺ efflux systems, is more tolerant to both glucose‐ and H₂O₂‐induced cell death. We hypothesize that ROS production, activated by glucose and H⁺‐ATPase and inhibited by K⁺ uptake, triggers leakage of K⁺, a process favoured by K⁺ efflux systems. Loss of cytosolic K⁺ probably causes osmotic lysis of vacuoles. The nature of the ROS‐producing system sensitive to K⁺ and H⁺ transport is unknown. Copyright © 2010 John Wiley & Sons, Ltd.     

Performance Analysis of Multiuser Detectors for Uplink Quasi-synchronous MC-CDMA Systems

This paper analyses and compares the average bit error rate (BER) of different multiuser detectors (MUD) in the uplink of a multicarrier code- division multiple access (MC-CDMA) system. In particular, maximum likelihood, zero-forcing, minimum mean-square error and interference cancellation-based multiuser detectors have been analysed for the special case of uncorrelated subcarriers. The derived BER expressions are based upon previous results on diversity combining and also on recent findings on multiple input multiple output (MIMO) architectures. The subcarrier correlation is considered in the context of physical parameters currently under discussion for future wireless systems to give an indication up to what extent the assumption of uncorrelated subcarrier fading is plausible.     

Evaluation of the surface urban heat island effect in the city of Madrid by thermal remote sensing

The surface urban heat island (SUHI) effect is defined as the increased surface temperatures in urban areas in contrast to cooler surrounding rural areas. In this article, the evaluation of the SUHI effect in the city of Madrid (Spain) from thermal infrared (TIR) remote-sensing data is presented. The data were obtained from the framework of the Dual-use European Security IR Experiment (DESIREX) campaign that was carried out during June and July 2008 in Madrid. The campaign combined the collection of airborne hyperspectral and in situ measurements. Thirty spectral and spatial high-resolution images were acquired with the Airborne Hyperspectral Scanner (AHS) sensor in a 11, 21, and 4 h UTC scheme. The imagery was used to retrieve the SUHI effect by applying the temperature and emissivity separation (TES) algorithm. The results show a nocturnal SUHI effect with a highest value of 5 K. This maximum value agrees within 1 K with the highest value of the urban heat island (UHI) observed using air temperature data (AT). During the daytime, this situation is reversed and the city becomes a negative heat island.