Alpha Fusion Adversarial Attack Analysis Using Deep Learning

The deep learning model encompasses a powerful learning ability that integrates the feature extraction, and classification method to improve accuracy. Convolutional Neural Networks (CNN) perform well in machine learning and image processing tasks like segmentation, classification, detection, identification, etc. The CNN models are still sensitive to noise and attack. The smallest change in training images as in an adversarial attack can greatly decrease the accuracy of the CNN model. This paper presents an alpha fusion attack analysis and generates defense against adversarial attacks. The proposed work is divided into three phases: firstly, an MLSTM-based CNN classification model is developed for classifying COVID-CT images. Secondly, an alpha fusion attack is generated to fool the classification model. The alpha fusion attack is tested in the last phase on a modified LSTM-based CNN (CNN-MLSTM) model and other pre-trained models. The results of CNN models show that the accuracy of these models dropped greatly after the alpha-fusion attack. The highest F1 score before the attack was achieved is 97.45 And after the attack lowest F1 score recorded is 22%. Results elucidate the performance in terms of accuracy, precision, F1 score and Recall.     

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

A transparent cornea is paramount for vision. Corneal opacity is one of the leading causes of blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, the outcomes are challenged by a global lack of donor tissue availability. Bioengineered corneal tissues are gaining momentum as a new source for corneal wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers a new perspective on corneal regenerative medicine. Ultrathin stromal laminar tissues obtained from lenticule-based refractive correction procedures, such as SMall Incision Lenticule Extraction (SMILE), are an accessible and novel source of collagen-rich ECM scaffolds with high mechanical strength, biocompatibility, and transparency. After customization (including decellularization), these lenticules can serve as an acellular scaffold niche to repopulate cells, including stromal keratocytes and stem cells, with functional phenotypes. The intrastromal transplantation of these cell/tissue composites can regenerate native-like corneal stromal tissue and restore corneal transparency. This review highlights the current status of ECM-scaffold-based engineering with cells, along with the development of drug and growth factor delivery systems, and elucidates the potential uses of stromal lenticule scaffolds in regenerative therapeutics.     

Regulation of the Fructose Transporter Gene Slc2a5 Expression by Glucose in Cultured Microglial Cells

Microglia play a role in the regulation of metabolism and pathogenesis of obesity. Microglial activity is altered in response to changes in diet and the body’s metabolic state. Solute carrier family 2 member 5 (Slc2a5) that encodes glucose transporter 5 (GLUT5) is a fructose transporter primarily expressed in microglia within the central nervous system. However, little is known about the nutritional regulation of Slc2a5 expression in microglia and its role in the regulation of metabolism. The present study aimed to address the hypothesis that nutrients affect microglial activity by altering the expression of glucose transporter genes. Murine microglial cell line SIM-A9 cells and primary microglia from mouse brain were exposed to different concentrations of glucose and levels of microglial activation markers and glucose transporter genes were measured. High concentration of glucose increased levels of the immediate-early gene product c-Fos, a marker of cell activation, Slc2a5 mRNA, and pro-inflammatory cytokine genes in microglial cells in a time-dependent manner, while fructose failed to cause these changes. Glucose-induced changes in pro-inflammatory gene expression were partially attenuated in SIM-A9 cells treated with the GLUT5 inhibitor. These findings suggest that an increase in local glucose availability leads to the activation of microglia by controlling their carbohydrate sensing mechanism through both GLUT5-dependent and –independent mechanisms.     

Combined Therapy Using Human Corneal Stromal Stem Cells and Quiescent Keratocytes to Prevent Corneal Scarring after Injury

Corneal blindness due to scarring is conventionally treated by corneal transplantation, but the shortage of donor materials has been a major issue affecting the global success of treatment. Pre-clinical and clinical studies have shown that cell-based therapies using either corneal stromal stem cells (CSSC) or corneal stromal keratocytes (CSK) suppress corneal scarring at lower levels. Further treatments or strategies are required to improve the treatment efficacy. This study examined a combined cell-based treatment using CSSC and CSK in a mouse model of anterior stromal injury. We hypothesize that the immuno-regulatory nature of CSSC is effective to control tissue inflammation and delay the onset of fibrosis, and a subsequent intrastromal CSK treatment deposited collagens and stromal specific proteoglycans to recover a native stromal matrix. Using optimized cell doses, our results showed that the effect of CSSC treatment for suppressing corneal opacities was augmented by an additional intrastromal CSK injection, resulting in better corneal clarity. These in vivo effects were substantiated by a further downregulated expression of stromal fibrosis genes and the restoration of stromal fibrillar organization and regularity. Hence, a combined treatment of CSSC and CSK could achieve a higher clinical efficacy and restore corneal transparency, when compared to a single CSSC treatment.     

Moisture management properties of plated knit structures with varying fiber types

Thermo-physiological comfort of clothing designed for next-to-skin applications is influenced by the clothing’s ability to manage heat and moisture transfer thereby maintaining dry skin microclimate. Plated knit structures designed and engineered with correct selection of fiber and yarn constituents in the distinct bottom (exposed to environment) and top (next to sin) layers can serve well for next-to-skin applications. In this study, plated fabrics with altering hydrophilic and hydrophobic fibers in top and bottom layers and different types of hydrophobic fibers in top layers have been compared for the moisture management properties. Results show that fabrics knitted with hydrophobic fibers (polypropylene, polyester) in top layers seem suitable for next-to-skin applications as they were classified as moisture management fabrics owing to high values of accumulative one-way transport index and bottom spreading speed. Though both fabrics can be recommended for next-to-skin applications, however, polypropylene on account of superior moisture management properties in the top layer would be more effective in providing dry feel next to skin and hence, seems to be a preferred choice over polyester for such applications. Fabric knitted with nylon in top layer was classified as water penetration fabric due to poor liquid transfer properties. Fabrics knitted with cotton in top layer irrespective of the hydrophobic fiber in bottom layer were poor in moisture management properties. Univariate analysis of variance with a confidence level of 95% showed the results to be statistically significant. Pearson correlation coefficient was obtained for all the moisture management indices by bivariate correlation procedure to determine strength and direction of association between the different moisture management indices. Most of the indices were found to be significantly correlated also, OWTC and OMMC were found to be positively and linearly related to each other.     

Thermal and mass transport properties of polyester–cotton plated fabrics in relation to back layer fibre profiles and face layer yarn types

Clothing plays an important role in maintaining thermal equilibrium between a human body and the ambient environment by serving as a medium for heat, moisture vapour and liquid moisture transfer. The ability of fabric to maintain this equilibrium is related to thermo-physiological comfort. Plating is an innovative knitted fabric production technique to obtain bi-layered fabrics. An attempt has been made to engineer plated knit structures with such a combination of fibre cross section in the back (inner/next to skin) and the yarn type in the face (outer) layer, so that a rapid liquid transfer from back layer by wicking and quick liquid absorption and evaporation by the face layer can be achieved. Plated fabrics using the combination of triangular polyester fibre in the back and carded cotton yarn in the face layer showed the higher thermal resistance, higher absorbent capacity and would be warmer to the initial touch. However, the combination of combed cotton yarn with triangular polyester fibre resulted in fabrics with the higher air permeability, moisture vapour transmission rate and transplanar wicking.     

Glucose Stimulates Glial Cell Line-Derived Neurotrophic Factor Gene Expression in Microglia through a GLUT5-Independent Mechanism

Feeding-regulating neurotrophic factors are expressed in both neurons and glial cells. However, nutritional regulation of anorexigenic glial cell line-derived neurotrophic factor (GDNF) and orexigenic mesencephalic astrocyte-derived neurotrophic factor (MANF) expression in specific cell types remains poorly understood. Hypothalamic glucose sensing plays a critical role in the regulation of food intake. It has been theorized that local glucose concentration modulates microglial activity partially via glucose transporter 5 (GLUT5). We hypothesized that an increased local glucose concentration stimulates GDNF expression while inhibiting MANF expression in the hypothalamus and microglia via GLUT5. The present study investigated the effect of glucose on Gdnf and Manf mRNA expression in the mouse hypothalamus and murine microglial cell line SIM-A9. Intracerebroventricular glucose treatment significantly increased Gdnf mRNA levels in the hypothalamus without altering Manf mRNA levels. Exposure to high glucose caused a significant increase in Gdnf mRNA expression and a time-dependent change in Manf mRNA expression in SIM-A9 cells. GLUT5 inhibitor treatment did not block glucose-induced Gdnf mRNA expression in these cells. These findings suggest that microglia are responsive to changes in the local glucose concentration and increased local glucose availability stimulates the expression of microglial GNDF through a GLUT5-independent mechanism, contributing to glucose-induced feeding suppression.