Survey of 1012 moldy dwellings by culture fungal analysis: Threshold proposal for asthmatic patient management

Different countries have tried to define guidelines to quantify what levels of fungi are considered as inappropriate for housing. This retrospective study analyzes indoor fungi by cultures of airborne samples from 1012 dwellings. Altogether, 908 patients suffering from rhinitis, conjunctivitis, and asthma were compared to 104 controls free of allergies. Portuguese decree law no 118/2013 (PDL118), ANSES (a French environmental and health agency) recommendations, and health regulations of Besançon University Hospital were applied to determine the rates of non‐conforming dwellings, which were respectively 55.2%, 5.2%, and 19%. Environmental microbiological results and medical data were compared. The whole number of colonies per cubic meter of air was correlated with asthma (P < 0.001) and rhinitis (P = 0.002). Sixty‐seven genera and species were detected in bedrooms. Asthma was correlated to Aspergillus versicolor (P = 0.004) and Cladosporium spp. (P = 0.02). Thresholds of 300 cfu/m³ for A. versicolor or 495 cfu/m³ for Cladosporium spp. are able to discriminate 90% of the asthmatic dwellings. We propose a new protocol to obtain an optimal cost for indoor fungi surveys, excluding surface analyses, and a new guideline to interpret the results based on >1000 cfu/m³ of whole colonies and/or above threshold levels for A. versicolor or Cladosporium spp.     

Genomics and Epigenomics of Gestational Diabetes Mellitus: Understanding the Molecular Pathways of the Disease Pathogenesis

One of the most common complications during pregnancy is gestational diabetes mellitus (GDM), hyperglycemia that occurs for the first time during pregnancy. The condition is multifactorial, caused by an interaction between genetic, epigenetic, and environmental factors. However, the underlying mechanisms responsible for its pathogenesis remain elusive. Moreover, in contrast to several common metabolic disorders, molecular research in GDM is lagging. It is important to recognize that GDM is still commonly diagnosed during the second trimester of pregnancy using the oral glucose tolerance test (OGGT), at a time when both a fetal and maternal pathophysiology is already present, demonstrating the increased blood glucose levels associated with exacerbated insulin resistance. Therefore, early detection of metabolic changes and associated epigenetic and genetic factors that can lead to an improved prediction of adverse pregnancy outcomes and future cardio-metabolic pathologies in GDM women and their children is imperative. Several genomic and epigenetic approaches have been used to identify the genes, genetic variants, metabolic pathways, and epigenetic modifications involved in GDM to determine its etiology. In this article, we explore these factors as well as how their functional effects may contribute to immediate and future pathologies in women with GDM and their offspring from birth to adulthood. We also discuss how these approaches contribute to the changes in different molecular pathways that contribute to the GDM pathogenesis, with a special focus on the development of insulin resistance.     

A comparative study of the tensile properties of compression molded and 3D printed PLA specimens in dry and water saturated conditions

Journal of Mechanical Science and Technology - This research work presents a comparative study of the tensile properties of polylactic acid (PLA) specimens per ASTM D638-14 fabricated by...     

Development of chemical sensors based on redox-dependent receptors: N,N'-dimethyldiazapyrenium-modified electrodes

Electrodes modified with Nafion films containing 2,7-dimethyldiazapyrenium (DAP2+) were prepared and characterized with voltammetry by themselves and in the presence of organic substrates. The large, planar, electron-poor aromatic surface in DAP2+ facilitates pi-stacking interactions with other planar aromatic molecules, particularly those that are negatively charged or electron-rich. Previous studies showed that the reduction of DAP2+ decreases the strength of these interactions, making the binding redox-dependent, and resulting in negative shifts in the E(1/2) of DAP2+/+. This study shows that the redox-dependent binding ability of DAP2+ is retained in Nafion, but the selectivity is considerably different. Most significantly, the electron-rich, neutral aromatic compounds that produced small shifts in the E(1/2) of DAP2+/+ in solution cause much larger shifts, up to -110 mV, with the modified electrodes. With indole as a substrate, Nernstian behavior is observed (-60 mV shift per log[indole]) between 10 and 0.5 mM.     

Investigation of the viscoelastic response of high temperature AS4‐12K/RP46 composites using a micromechanical approach

The viscoelastic behavior of a RP46 polyimide resin is characterized at high temperature and the results are used within a micromechanical model to predict the viscoelastic response of a RP46 based carbon fiber composite. The creep master curve of the neat resin is obtained using the time temperature superposition principle (TTSP) from creep tests at three different temperatures, namely 180, 220, and 270°C. The viscoelastic behavior of RP46 is modeled based on Schapery's single integral constitutive equation whose Prony Series coefficients are obtained from the master curve. The acquired properties are then incorporated into a Simplified Unit Cell Micromechanical model to study the creep response of a RP46 resin based composite system. The advantage of this particular micromechanical model lies in its ability to give closed form expressions for the effective viscoelastic response of unidirectional composites as well as each of their constituents. Two types of nonlinearities were observed, one due to stress and the other due to temperature. Both of these nonlinearities can be modeled through the use of proper coefficients in the constitutive equation of the matrix material. The model predictions are found to be in good agreement with experimental results obtained from tests conducted on the RP46 resin based composite system. POLYM. COMPOS., 37:1407–1414, 2016. © 2014 Society of Plastics Engineers     

An investigation of the damage mechanisms and fatigue life diagrams of flax fiber-reinforced polymer laminates

In this paper we investigated the fatigue damage of a unidirectional flax-reinforced epoxy composite using infrared (IR) thermography. Two configurations of flax/epoxy composites layup were studied namely, [0]₁₆ unidirectional ply orientation and [±45]₁₆. The high cycle fatigue strength was determined using a thermographic criterion developed in a previous study. The fatigue limit obtained by the thermographic criterion was confirmed by the results obtained through conventional experimental methods (i.e., Stress level versus Number of cycles to failure). Furthermore, a model for predicting the fatigue life using the IR thermography was evaluated. The model was found to have a good predictive value for the fatigue life. In order to investigate the mechanism of damage initiation in flax/epoxy composites and the damage evolution, during each fatigue test we monitored the crack propagation for a stress level and at different damage stages, a direct correlation between the percentage of cracks and the mean strain was observed.     

A micromechanical approach for the prediction of the time-dependent failure of high temperature polymer matrix composites

In the present study, a novel micromechanical approach is introduced to study the time-dependent failure of unidirectional polymer matrix composites. The main advantage of the present micromechanical model lies in its ability to give closed-form solutions for the effective nonlinear response of unidirectional composites and to predict the material response to any combination of shear and normal loading. The creep failure criterion is expressed in terms of the creep failure functions of the viscoelastic matrix material. The micromechanical model is also used to calculate these creep failure functions from the knowledge of the creep behavior of the composite material in only transverse and shear loadings, thus eliminating the need for any further experimentation. The composite material used in this study is T300/934, which is suitable for service at high temperatures in aerospace applications. The use of micromechanics can give a more accurate insight into the failure mechanisms of the composite materials in particular at high temperatures where the general behavior of the polymer matrix composite is governed by matrix viscoelasticity and the time-dependent failure of the matrix is a localized phenomenon. The obtained creep failure stresses are found to be in reasonable agreement with the experimental data.     

Convection in a vertical cavity submitted to crossed uniform heat fluxes

In this study, free convection in a vertical cavity heated from the four walls by uniform heat fluxes is considered. Analytical solutions are derived for a fully developed base flow, for which linear stability analysis predicts the growth of oblique, three-dimensional disturbances in general. A Hopf type bifurcation occurs at the critical Rayleigh number, over the entire range of Prandtl numbers and heat flux ratios considered, characterized by oscillating instabilities. Depending mostly on the value of the Prandtl number, either thermal, for Pr>1, or hydrodynamic, for Pr<1, instability modes are predicted. For small Prandtl numbers, both modes can occur at the codimension two intersection points of the critical branches.     

The biomechanics of a validated finite element model of stress shielding in a novel hybrid total knee replacement

This study proposes a novel hybrid total knee replacement (TKR) design to improve stress transfer to bone in the distal femur and, thereby, reduce stress shielding and consequent bone loss. Three-dimensional finite element (FE) models were developed for a standard and a hybrid TKR and validated experimentally. The Duracon knee system (Stryker Canada) was the standard TKR used for the FE models and for the experimental tests. The FE hybrid device was identical to the standard TKR, except that it had an interposing layer of carbon fibre-reinforced polyamide 12 lining the back of the metallic femoral component. A series of experimental surface strain measurements were then taken to validate the FE model of the standard TKR at 3000 N of axial compression and at 0 degreeof knee flexion. Comparison of surface strain values from FE analysis with experiments demonstrated good agreement, yielding a high Pearson correlation coefficient of R(2)= 0.94. Under a 3000N axial load and knee flexion angles simulating full stance (0O degree, heel strike (200 degrees, and toe off (600 degrees during normal walking gait, the FE model showed considerable changes in maximum Von Mises stress in the region most susceptible to stress shielding (i.e. the anterior region, just behind the flange of the femoral implant). Specifically, going from a standard to a hybrid TKR caused an increase in maximum stress of 87.4 per cent (O0 degree from 0.15 to 0.28 MPa), 68.3 per cent (200 degrees from 1.02 to 1.71 MPa), and 12.6 per cent (600 degrees from 2.96 to 3.33 MPa). This can potentially decrease stress shielding and subsequent bone loss and knee implant loosening. This is the first report to propose and biomechanically to assess a novel hybrid TKR design that uses a layer of carbon fibrereinforced polyamide 12 to reduce stress shielding.     

An Overview of the Approaches for Automotive Safety Integrity Levels Allocation

Journal of Failure Analysis and Prevention - ISO 26262, titled Road Vehicles–Functional Safety, is the new automotive functional safety standard for passenger vehicle industry. In order to...