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排序方式: 共有666条查询结果,搜索用时 15 毫秒
1.
Yuan-Cheng Meng Hua-Feng Zhang Xiao-Xiao Pan Nan Chen Hui-Fang Hu Saeed ul Haq Abid Khan Ru-Gang Chen 《International journal of molecular sciences》2021,22(6)
Dehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a Y1SK2-type DHN gene “CaDHN3” from pepper. To authenticate the function of CaDHN3 in salt and drought stresses, it was overexpressed in Arabidopsis and silenced in pepper through virus-induced gene silencing (VIGS). Sub-cellular localization showed that CaDHN3 was located in the nucleus and cell membrane. It was found that CaDHN3-overexpressed (OE) in Arabidopsis plants showed salt and drought tolerance phenotypic characteristics, i.e., increased the initial rooting length and germination rate, enhanced chlorophyll content, lowered the relative electrolyte leakage (REL) and malondialdehyde (MDA) content than the wild-type (WT) plants. Moreover, a substantial increase in the activities of antioxidant enzymes; including the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and lower hydrogen peroxide (H2O2) contents and higher O2•− contents in the transgenic Arabidopsis plants. Silencing of CaDHN3 in pepper decreased the salt- and drought-stress tolerance, through a higher REL and MDA content, and there was more accumulation of reactive oxygen species (ROS) in the CaDHN3-silenced pepper plants than the control plants. Based on the yeast two-hybrid (Y2H) screening and Bimolecular Fluorescence Complementation (BiFC) results, we found that CaDHN3 interacts with CaHIRD11 protein in the plasma membrane. Correspondingly, the expressions of four osmotic-related genes were significantly up-regulated in the CaDHN3-overexpressed lines. In brief, our results manifested that CaDHN3 may play an important role in regulating the relative osmotic stress responses in plants through the ROS signaling pathway. The results of this study will provide a basis for further analyses of the function of DHN genes in pepper. 相似文献
2.
Rizwan Ullah Khan Haojie Yu Li Wang Lisong Teng Zain-ul-Abdin Ahsan Nazir Shah Fahad Tarig Elshaarani Fazal Haq Di Shen 《应用聚合物科学杂志》2020,137(46):49424
The development of safe drug carriers is cardinal in cancer therapy, which can target the cancer cells and release the loaded drug on-demand without damaging the healthy cells of the body. In our work, we synthesized three different biodegradable polymers, poly[(ethyl aminobezoate) (ethyl glycinato) phosphazenes] (PABGPs), in different mole ratio of side groups. The successful synthesis of these PABGPs was confirmed by 1H NMR, 31P NMR, FT-IR, and gel permeation chromatography. These PABGPs were fabricated into drug (camptothecin, CPT, a hydrophobic anticancer drug) loaded nanoparticles. These drug-loaded nanoparticles showed good drug release behaviors under normal physiological conditions (pH 7.4 and temperature 37°C). These PABGPs-based nanoparticles may find their application as effective drug carriers for cancer therapy. 相似文献
3.
Fine particles of cadmium carbonate (CdCO3) were prepared by heating aqueous solutions containing dissolved urea and cadmium sulfate at 85°C for 1 h. Effect of the composition of the reactant mixture on the morphology of the precipitated particles was investigated. It was found that the cadmium sulfate content of the reactant mixture significantly affected the shape and size of the precipitated particles. At certain range of reactant mixture composition, cauliflower-shaped uniform particles were obtained. The later particles were coated with basic copper carbonate (Cu2(OH)2CO3) by heating aqueous dispersions, containing CdCO3 particles, copper (II) nitrate, and urea at 85°C for various periods of time with constant stirring. The coating process was found sensitive to the experimental conditions and in most of the trials; mixtures of the coated and coating precursor particles were obtained. As such, extensive optimizations were carried out and conditions were established for the production of uniformly coated particles.On calcination at 700°C for 1 h, the CdCO3 particles converted into CdO, whereas the coated particles (CdCO3
(core)/Cu2(OH)2CO3
(coating) transformed into CdO(core)/CuO(coating)) without sintering. In the coated particles, the core and coating materials stayed mutually inert during the calcination reaction and independently converted into their respective oxides.All the calcined and uncalcined products were characterized by various physical and chemical methods. 相似文献
4.
Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene 总被引:3,自引:0,他引:3
The authors have designed and constructed a plant-optimize synthetic gene encoding the Escherichia coli heat-labile enterotoxin B subunit (LT-B), for use in transgenic plants as an edible vaccine against enterotoxigenic E. coli. Expression of the synthetic LT-B gene in potato plants under the control of a constitutive promoter yielded increased accumulation of LT-B in leaves and tubers, as compared to the bacterial LT-B gene. The plant-derived LT-B assembled into native pentameric structures as evidenced by its ability to bind ganglioside. The authors demonstrated immunogenicity by feeding mice the raw tubers and comparing the anti-LT-B serum IgG and faecal IgA to that produced in mice gavaged with bacterial LT-B. Mice were fed three weekly doses of 5 g tuber tissue containing either 20 or 50 micrograms LT-B, or gavaged weekly with 5 micrograms of LT-B from recombinant E. coli. One week after the third dose, mice immunized with potato LT-B had higher levels of serum and mucosal anti-LT-B than those gavaged with bacterial LT-B. Mice were challenged by oral administration of 25 micrograms LT, and protection assessed by comparing the gut/carcass mass ratios. Although none of the mice were completely protected, the higher dose potato vaccine compared favourably with the bacterial vaccine. These findings show that an edible vaccine against E. coli LT-B is feasible. 相似文献
5.
Khalid Mahmood Zia Mehdi Barikani Ijaz Ahmad Bhatti Mohammad Zuber Haq Nawaz Bhatti 《应用聚合物科学杂志》2008,109(3):1840-1849
A series of polyurethane (PU) elastomers was prepared by the reaction of poly(?‐caprolactone) and 4,4′‐diphenylmethane diisocyanate, which was extended with a series of chain extenders (CEs) having 2–10 methylene units in their structure. The completion of the reaction was confirmed by Fourier transform infrared spectroscopy. The chemical structures of the synthesized PU samples were characterized with Fourier transform infrared, 1H‐NMR, and 13C‐NMR spectroscopy, and the thermal properties were determined by thermogravimetric analysis, DSC, and dynamic mechanical thermal analysis techniques. The mechanical properties were also studied and are discussed. The thermogravimetric analysis and DSC analysis showed that CE length had a considerable effect on the thermal properties of the prepared samples. The dynamic mechanical thermal analysis and damping peaks were also affected by the number of methylene units in the CE length. The elastomer extended with 1,2‐ethane diol exhibited optimum thermal properties, whereas the elastomer based on 1,10‐decane diol displayed the worst thermal properties. Tensile strength and elongation at break decreased with increasing CE length, whereas hardness showed the opposite trend. The glass‐transition temperature moved toward lower temperatures with increasing CE length. The decrease in the glass‐transition temperature and tensile properties were interpreted in terms of decreasing hard segments and increasing chain flexibility. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008 相似文献
6.
Tae-Sung Jung Do-Chan Choi Sung-Hee Cho Myong-Jae Kim Seung-Keun Lee Byung-Soon Choi Jin-Sun Yum San-Hong Kim Dong-Gi Lee Jong-Chang Son Myung-Sik Yong Heung-Kwun Oh Sung-Bu Jun Woung-Moo Lee Haq E. Kang-Deog Suh Ali S.B. Hyung-Kyu Lim 《Solid-State Circuits, IEEE Journal of》1997,32(11):1748-1757
A 3.3-V 16-Mb nonvolatile memory having operation virtually identical to DRAM with package pin compatibility has been developed. Read and write operations are fully DRAM compatible except for a longer RAS precharge time after write. Fast random access time of 63 ns with the NAND flash memory cell is achieved by using a hierarchical row decoder scheme and a unique folded bit-line architecture which also allows bit-by-bit program verify and inhibit operation. Fast page mode with a column address access time of 21 ns is achieved by sensing and latching 4 k cells simultaneously. To allow byte alterability, nonvolatile restore operation with self-contained erase is developed. Self-contained erase is word-line based, and increased cell disturb due to the word-line based erase is relaxed by adding a boosted bit-line scheme to a conventional self-boosting technique. The device is fabricated in a 0.5-μm triple-well, p-substrate CMOS process using two-metal and three-poly interconnect layers. A resulting die size is 86.6 mm2, and the effective cell size including the overhead of string select transistors is 2.0 μm2 相似文献
7.
We present a spectral rendering technique that offers a compelling set of advantages over existing approaches. The key idea is to propagate energy along paths for a small, constant number of changing wavelengths. The first of these, the hero wavelength, is randomly sampled for each path, and all directional sampling is solely based on it. The additional wavelengths are placed at equal distances from the hero wavelength, so that all path wavelengths together always evenly cover the visible range. A related technique, spectral multiple importance sampling, was already introduced a few years ago. We propose a simplified and optimised version of this approach which is easier to implement, has good performance characteristics, and is actually more powerful than the original method. Our proposed method is also superior to techniques which use a static spectral representation, as it does not suffer from any inherent representation bias. We demonstrate the performance of our method in several application areas that are of critical importance for production work, such as fidelity of colour reproduction, sub‐surface scattering, dispersion and volumetric effects. We also discuss how to couple our proposed approach with several technologies that are important in current production systems, such as photon maps, bidirectional path tracing, environment maps, and participating media. 相似文献
8.
An efficient numerical algorithm for the study of time fractional Tricomi and Keldysh type equations
Ghafoor Abdul Haq Sirajul Rasool Amir Baleanu Dumitru 《Engineering with Computers》2022,38(4):3185-3195
Engineering with Computers - This work addresses a hybrid scheme for the numerical solutions of time fractional Tricomi and Keldysh type equations. In proposed methodology, Haar wavelets are used... 相似文献
9.
Huay Woon You Michael B. C. Khoo Philippe Castagliola Abdul Haq 《Quality and Reliability Engineering International》2016,32(5):1913-1927
Many quality characteristics have means and standard deviations that are not independent. Instead, the standard deviations of these quality characteristics are proportional to their corresponding means. Thus, monitoring the coefficient of variation (CV), for these quality characteristics, using a control chart has gained remarkable attention in recent years. This paper presents a side sensitive group runs chart for the CV (called the SSGR CV chart). The implementation and optimization procedures of the proposed chart are presented. Two optimization procedures are developed, i.e. (i) by minimizing the average run length (ARL) when the shift size is deterministic and (ii) by minimizing the expected average run length (EARL) when the shift size is unknown. An application of the SSGR CV chart using a real dataset is also demonstrated. Additionally, the SSGR CV chart is compared with the Shewhart CV, runs rules CV, synthetic CV and exponentially weighted moving average CV charts by means of ARLs and standard deviation of the run lengths. The performance comparison is also conducted using EARLs when the shift size is unknown. In general, the SSGR CV chart surpasses the other charts under comparison, for most upward and downward CV shifts. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
10.
Jesmin Haq Bryan D. Vogt Emmett Howard Doug Loy 《Journal of the Society for Information Display》2010,18(11):884-891
Abstract— A processing technology based upon a temporary bond—debond approach has been developed that enables direct fabrication of high‐performance electronic devices on flexible substrates. This technique facilitates processing of flexible plastic and metal‐foil substrates through automated standard semiconductor and flat‐panel tool sets without tool modification. The key to processing with these tool sets is rigidifying the flexible substrates through temporary bonding to carriers that can be handled in a similar manner as silicon wafers or glass substrates in conventional electronics manufacturing. To demonstrate the power of this processing technology, amorphous‐silicon thin‐film‐transistor (a‐Si:H TFT) backplanes designed for electrophoretic displays (EPDs) were fabricated using a low‐temperature process (180°C) on bonded‐plastic and metal‐foil substrates. The electrical characteristics of the TFTs fabricated on flexible substrates are found to be consistent with those processed with identical conditions on rigid silicon wafers. These TFTs on plastic exhibit a field‐effect mobility of 0.77 cm2/V‐sec, on/off current ratio >109 at Vds = 10 V, sub‐threshold swing of 365 mV/dec, threshold voltage of 0.49 V, and leakage current lower than 2 pA/μm gate width. After full TFT‐array fabrication on the bonded substrate and subsequent debonding, the flexible substrate retains its original flexibility; this enables bending of the EPD display without loss in performance. 相似文献